Mass spectrometric analysis of integral membrane proteins: Application to complete mapping of bacteriorhodopsins and rhodopsin

Protein Science

Volumn 7, Issue 3, 1998, Pages 758-764

  Ball, Lauren E ^a   Oatis Jr, John E ^a   Dharmasiri, Kuruppu ^a   Busman, Mark ^a,b   Wang, Jianyao ^c   Cowden, Lloraine B ^a   Galijatovic, Alema ^a   Chen, Ning ^a   Crouch, Rosalie K ^a   Knapp, Daniel R ^a  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b National Marine Fisheries Service   (United States)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

Bacteriorhodopsin; Electrospray; G protein coupled receptor; HPLC; Integral membrane proteins; Mass spectrometry; Rhodopsin

Indexed keywords

BACTERIORHODOPSIN; CYANOGEN BROMIDE; MEMBRANE PROTEIN; RHODOPSIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CATTLE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PEPTIDE MAPPING; PRIORITY JOURNAL; PROTEIN DEGRADATION; RETINA ROD OUTER SEGMENT; TANDEM MASS SPECTROMETRY;

EID: 0031887346     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070325     Document Type: Article

References (21)

1. Allmaier G, Chao BH, Khorana HG, Biemann K. 1986. The determination of the location and nature of amino acid substitutions in biosynthetic bacterioopsin. Proceeding of the 34th annual conference on mass spectrometry and allied topics. Cincinnati, OH: American Society for Mass Spectrometry. pp 308-309.
2. Bairoch A, Apweiler R. 1996. The SWISS-PROT protein sequence data bank and its new supplement TREMBL. Nucl Acids Res 24:21-25.
3. Balashov SP, Govindjee R, Kono M, Imasheva E, Lukashov E, Ebrey TG, Crouch RK, Menick DR, Feng Y. 1993. Effect of the arginine-82 to alanine mutation in bacteriorhodopsin on dark adaptation, proton release and the photochemical cycle. Biochemistry 32:10331-10343.
4. Balashov SP, Imasheva ES, Ebrey TG, Chen N, Menick DR, Crouch RK. 1997. Glutamate-194 to cysteine mutation inhibits fast light-induced proton release in bacteriorhodopsin. Biochemistry 36:8671-8676.
5. Barnidge DR, Dratz EA, Sunner J, Jesaitis AJ. 1997. Identification of transmembrane tryptic peptides of rhodopsin using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. Protein Sci 6:816-824.
6. Biemann K. 1990. Nomenclature for peptide fragment ions. Meth Enzymol 193:886-887.
7. Busman M, Wang J, Cowden LB, Oatis JE Jr, Chen N, Crouch RK, Knapp DR. 1996. Analysis of bacteriorhodopsin cleavage fragments by HPLC-ESI-MS and MS/MS. Proceeding of the 44th annual conference on mass spectrometry and allied topics. Palm Springs, CA: American Society for Mass Spectrometry. p 653.
8. Duffin KL, Lang GW, Welpy JK, Florman R, O'Brien PJ, Dell A, Reason AJ, Morris HJ, Fliesler SJ. 1993. Identification and oligosaccharide structure analysis of rhodopsin glycoforms containing galactose and sialic acid. Glycobiology 3:365-380.
9. Hargrave PA, McDowell JH, Feldmann RJ, Atkinson PH, Mohana Rao JK, Argos P. 1984. Rhodopsin's protein and carbohydrate structure: Selected aspects. Vision Res 24:1487-1499.
10. Karger BL, Hancock WS, Eds. 1996. High resolution separation and analysis of biological macromolecules, methods in enzymology. San Diego: Academic Press.
11. Knapp DR, Oatis JE Jr, Papac DI. 1993. Small-scale manual multiple peptide synthesis system. Int J Pep Prot Res 42:259-263.
12. Lerro KL, Orlando R, Zhang H, Usherwood PN, Nakanishi K. 1993. Separation of the sticky peptides from membrane proteins by high-performance liquid chromatography in a normal-phase system. Anal Biochem 215:38-44.
13. McCloskey JA, ed. 1990. Methods in enzymology 193. San Diego: Academic Press.
14. McDowell JH, Kühn H. 1977. Light-induced phosphorylation of rhodopsin in cattle photoreceptor membranes: Substrate activation and inactivation. Biochemistry 16:4054-4060.
15. Oesterhelt D, Stoekenius A. 1974. Isolation of the cell membrane of halobacterium halobium and its fractionation into red and purple membrane. Meth Enzymol 31:667-678.
16. Orlando R, Kenny PTM, Moquin-Pattey C, Lerro KA, Nakanishi K. 1993. Sequencing membrane proteins by mass spectrometry. Org Mass Spectrom 28:1395-1402.
17. Ovchinnikov YA, Abdulaev NG, Feigina MY, Artamov ID, Zolotarev AS, Kostina MB, Bogachuk AS, Miroshnikov AI, Martinov VI, Kudelin AB. 1982. The complete amino acid sequence of visual rhodopsin. Bioorg Khim 8:1011-1014.
18. Papac DI. 1993. Structural analysis of an intrinsic membrane protein using mass spectrometry, rhodopsin as a model case [Ph.D. dissertation]. Charleston: Medical University of South Carolina.
19. Shively JE. 1994. Micromethods for protein structure analysis. In: Shively JE, ed. Methods 6. San Diego: Academic Press. pp 207-212.
20. Tarr GE, Crabb JW. 1983. Reverse-phase high-performance liquid chromatography of hydrophobic proteins and fragments thereof. Anal Biochem 131: 99-107.
21. Watson S, Arkinstall S. 1994. The G-protein coupled receptor facts book. New York: Academic Press.