Identification of transmembrane tryptic peptides of rhodopsin using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Protein Science

Volumn 6, Issue 4, 1997, Pages 816-824

  Barnidge, David R ^a   Dratz, Edward A ^a   Sunner, Jan ^a   Jesaitis, Algirdas J ^a  

^a MONTANA STATE UNIVERSITY   (United States)

Author keywords

G protein coupled receptor; hydrophobic peptide; mass spectrometry; reverse phase HPLC; rhodopsin

Indexed keywords

GLUCOSIDE; GUANINE NUCLEOTIDE BINDING PROTEIN; MEMBRANE PROTEIN; MEMBRANE RECEPTOR; PEPTIDE; RHODOPSIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING SITE; CONSENSUS SEQUENCE; DESORPTION; GLYCOSYLATION; IONIZATION; LIGAND BINDING; LIPID BILAYER; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; TOPOGRAPHY;

EID: 0031004233     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060408     Document Type: Article

References (34)

1. Baldwin JM. 1993. The probable arrangement of the helices in G protein-coupled receptors. EMBO J 12:1693-1703
2. Baldwin JM. 1994. Structure and function of receptors coupled to G proteins. Curr Opin Cell Biol 6:180-190.
3. Baldwin JM, Henderson R, Beckmann E, Zemlin F. 1988. Images of purple membrane at 2.8 Å resolution obtained by cryo-electron microscopy. J Mol Biol 202:585-591.
4. Bull HB, Breese K. 1974. Hydrophobicity estimates for proteins and peptides. Arch Biochem Biophys 161:665-670.
5. Burritt JB, Quinn MT, Jutila MA, Bond CW, Jesaitis AJ. 1995. Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries. J Biol Chem 270:16974-16980.
6. De Grip WJ. 1982. Purification of bovine rhodopsin over concanavalin A-sepharose. In: Packer L. ed. Methods in enzymology, vol 81. New York: Academic Press, pp 197-207.
7. Deisenhofer J, Michel H. 1989. The photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis. Biosci Rep 9:383-419.
8. Dorman G, Prestwich GD. 1994. Benzophenone photophores in biochemistry. Biochem 33:5661-5673.
9. Hargrave PA, McDowell JH, Curtis DR, Wang JK, Juszczak E, Fong S-L, Rao JK, Argos P. 1983. The structure of bovine rhodopsin. Biophys Structr Mech 9:235-244.
10. Hemling ME, Carr SA, Capiau C, Petre J. 1988. Structural characterization of recombinant hepatitis B surface antigen protein by mass spectrometry. Biochem 27:699-705.
11. Jennings DN. 1989. Methods for structural analysis of membrane proteins. Annu Rev Biochem 58:999-1027.
12. Kean EL, Hara S, Mizoguchi A, Matsumoto A, Kobata A. 1983. The enzymatic cleavage of rhodopsin by the retinal pigment epithelium II. The carbohydrate composition of the glycopeptide cleavage product. Exp Eye Res 36:817-825.
13. 2+ ATPase. Anal Biochem 214:50-57.
14. Liang C-J, Yamashita K, Muellenberg CG, Shichi H, Kobata A, 1979. Structure of the carbohydrate moieties of bovine rhodopsin. J Biol Chem 254:6414-6418.
15. Machold J, Utkin Y, Kirsch D, Kaufmann R, Tsetlin V, Hucho F. 1995. Photolabeling reveals the proximity of the alpha-neurotoxin binding site to the M2 helix of the ion channel in the nicotinic acetylcholine receptor. Proc Natl Acad Sci USA 92:7282-7286.
16. Miljanich GP, Brown MF, Mabrey-Gaud S, Dratz EA, Sturtevant JM. 1985. Thermotropic behavior of retinal rod membranes and dispersions of extracted phospholipids. J Membr Biol 85:79-86.
17. Moore CR, Yates JR, Griffin PR, Shabanowitz J, Martino PA, Hunt DF, Cafiso DS. 1989. Proteolytic fragments of the nicotinic acetylcholine receptor identified by mass spectrometry: Implications for receptor topography. Biochem 28:9148-9191.
18. Nakanishi S, Zang H, Lerro KA, Takekuma S, Yamamoto T, Lien TH, Sastry L, Baek D-J, Moquin-Pattey C, Boehm MF. 1996. Photoaffinity labeling of rhodopsin and bacteriorhodopsin. Biophys Chem 56:13-22.
19. Nakayama TA, Khorona HG. 1990. Orientation of retinal in bovine rhodopsin determined by cross-linking using a photoactivatable analog of 11-cis-retinal. J Biol Chem 265:15762-15769.
20. Orlando R, Kenny PTM, Moquin-Pattey C, Lerro KA, Nakanishi K. 1993. Sequencing membrane proteins by tandem mass spectrometry. Org Mass Spectrom 25:1395-1402.
21. Plantner JJ, Kean EL. 1976. Carbohydrate composition of bovine rhodopsin. J Biol Chem 251:1548-1552.
22. Plantner JJ, Le ML, Kean EL. 1991. Enzymatic deglycosylation of bovine rhodopsin. Exp Eye Res 53:269-274.
23. Poulter L, Earnest JP, Stroud RM, Burlingame AL. 1989. Structure, oligosaccharide structures, and posttranslationally modified sites of the nicotinic acetylcholine receptor. Proc Natl Acad Sci USA 86:6645-6649.
24. Rosinke B, Strupat K, Hillenkamp F, Rosenbusch J, Dencher N, Kruger U, Galla HJ. 1995. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of membrane proteins and non-covalent complexes. J Mass Spectrom 30:1462-1468.
25. Schertler GF, Villa C, Henderson R. 1993. Projection structure of rhodopsin. Nature 362:770-772.
26. Schertler GF, Hargrave PA. 1995. Projection structure of frog rhodopsin in two crystal forms. Proc Natl Acad Sci USA 92:11578-11582.
27. Shoelson SE, Lee J, Lynch JM, Backer JM, Pilch PF. 1993. BpaB25 insulins: Photoactivatable analogs that quantitatively cross-link, radiolabel, and activate the insulin receptor. J Biol Chem 268:4085-4091.
28. Stone KL, Lo Presti M, Crawford J, DeAngelis R, Williams K. 1989. Enzymatic digestion of proteins and HPLC peptide isolation. In: Matsudaira PT, ed. A practical guide to protein and peptide purification for microsequencing. San Diego, California: Academic Press. pp 31-47.
29. Tomich JM, Carson LW, Kanes KJ, Vogelaar NJ, Emerling MR, Richards JH. 1988. Prevention of aggregation of synthetic membrane-spanning peptides by addition of detergent. Anal Biochem 174:197-203.
30. Tsukihara T, Aoyama H, Yamashita E, Tomizaki T, Yamaguchi H, Shinzawa Itoh K, Nakashima R, Yaono R, Yoshikawa S. 1996. The whole structure of the 13-subunit oxidized cytochrome c oxidase at 2.8 Å. Science 272:1136-1144.
31. Unger VM, Schertler, GF. 1995. Low resolution structure of bovine rhodopsin determined by electron cryo-microscopy. Biophys J 68:1776-1786.
32. Wang JK, McDowell J H, Hargrave PA. 1980. Site of attachment of 11-cis-retinal in bovine rhodopsin. Biochemistry 19:5111-5117.
33. Williams KP, Shoelson SE. 1993. A photoaffinity scan maps regions of the p85 SH2 domain involved in phosphoprotein binding. J Biol Chem 268:5361-5364.
34. Wilmanns M, Lappalainen P, Kelly M, Sauer-Eriksson E, Saraste M. 1995. Crystal structure of the membrane-exposed domain from a respiratory quinol oxidase complex with an engineered dinuclear copper center. Proc Natl Acad Sci USA 92:11955-11959.