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Volumn 272, Issue 5262, 1996, Pages 725-728

Homologous association of oppositely imprinted chromosomal domains

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CYCLE S PHASE; CHROMOSOME 15Q; CHROMOSOME ANALYSIS; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GENE LOCUS; GENOME IMPRINTING; HAPPY PUPPET SYNDROME; HUMAN; HUMAN CELL; PRADER WILLI SYNDROME; PRIORITY JOURNAL; T LYMPHOCYTE;

EID: 0030043993     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.272.5262.725     Document Type: Article
Times cited : (204)

References (37)
  • 1
    • 0000135026 scopus 로고
    • H V Crouse, Genetics 45, 1429 (1960); A Razin and H. Cedar, Cell 77, 473 (1994)
    • (1960) Genetics , vol.45 , pp. 1429
    • Crouse, H.V.1
  • 2
    • 0028286005 scopus 로고
    • H V Crouse, Genetics 45, 1429 (1960); A Razin and H. Cedar, Cell 77, 473 (1994)
    • (1994) Cell , vol.77 , pp. 473
    • Razin, A.1    Cedar, H.2
  • 4
    • 0027205671 scopus 로고
    • D. Kitsberg et al , Nature 364, 459 (1993).
    • (1993) Nature , vol.364 , pp. 459
    • Kitsberg, D.1
  • 5
    • 0027980355 scopus 로고
    • M Lalande, Nature Genet 8, 5(1994), R. D Nicholls, J. H. M. Knoll, M. G. Butler, S. Karam, M Lalande, Nature 342, 281 (1989), S. Malcolm et al., Lancet 337, 694 (1991)
    • (1994) Nature Genet , vol.8 , pp. 5
    • Lalande, M.1
  • 7
    • 0026080417 scopus 로고
    • M Lalande, Nature Genet 8, 5(1994), R. D Nicholls, J. H. M. Knoll, M. G. Butler, S. Karam, M Lalande, Nature 342, 281 (1989), S. Malcolm et al., Lancet 337, 694 (1991)
    • (1991) Lancet , vol.337 , pp. 694
    • Malcolm, S.1
  • 10
    • 0027391819 scopus 로고
    • Y. Hiraoka et al , J. Cell Biol. 120, 591 (1993); B Judd, Cell 53, 841 (198 8 ) , V Pirrotta, BioEssays 12, 409 (1990); K. D Tartof and S. Henikoff, Cell 65, 201 (1991).
    • (1993) J. Cell Biol. , vol.120 , pp. 591
    • Hiraoka, Y.1
  • 11
    • 0024290485 scopus 로고
    • Y. Hiraoka et al , J. Cell Biol. 120, 591 (1993); B Judd, Cell 53, 841 (198 8 ) , V Pirrotta, BioEssays 12, 409 (1990); K. D Tartof and S. Henikoff, Cell 65, 201 (1991).
    • (1988) Cell , vol.53 , pp. 841
    • Judd, B.1
  • 12
    • 0025487089 scopus 로고
    • Y. Hiraoka et al , J. Cell Biol. 120, 591 (1993); B Judd, Cell 53, 841 (198 8 ) , V Pirrotta, BioEssays 12, 409 (1990); K. D Tartof and S. Henikoff, Cell 65, 201 (1991).
    • (1990) BioEssays , vol.12 , pp. 409
    • Pirrotta, V.1
  • 13
    • 0025857244 scopus 로고
    • Y. Hiraoka et al , J. Cell Biol. 120, 591 (1993); B Judd, Cell 53, 841 (198 8 ) , V Pirrotta, BioEssays 12, 409 (1990); K. D Tartof and S. Henikoff, Cell 65, 201 (1991).
    • (1991) Cell , vol.65 , pp. 201
    • Tartof, K.D.1    Henikoff, S.2
  • 15
    • 13344263593 scopus 로고    scopus 로고
    • note
    • 4 fractions (6). A modified FISH protocol was developed that minimizes alterations in the normal spatial organization of the nucleus (21) Cells were adhered for 5 min to poly-D-lysine-coated slides, fixed 15 min in 4% paraformaldehyde and 1% methanol in phosphate-buffered saline (PBS), washed twice in 0 3 M glycine and PBS, permeabilized for 10 min in 0 5% Tween and 0.2 N HCl, washed twice in 2x saline sodium citrate (SSC), denatured 3 min in 70% tormamide and 2x SSC, washed twice in 0.5% Tween and PBS and twice in 2x SSC at 4°C, then hybridized and washed as previously described (6) without detection steps D15Z1 (pHSR) and D12Z3 (pA12H8, ATCC) were directly labeled by nick translation with Cy3- and Cy5-labeled deoxycytidine triphosphate (Biological Detection), respectively, and nuclei were counterstained with YOPRO (Molecular Probes). Nuclei were imaged by a Molecular Dynamics CLSM Multiprobe 2001 with an Ar/Kr laser. Imagespace software (Molecular Dynamics) was used for scanning, analysis, and projection images Nuclei were densely distributed (Fig. 1A), and we selected clusters of 6 to 20 nuclei by using only the DNA counterstain filter to avoid bias in selection. Optical sections were scanned with a 60x objective at a 0.21, 0.21, 0.29 voxel resolution and a total image size of 512 by 512 pixels. Hybridization efficiency was 85% (number of nuclei with two red and two green signals out of the total number of nuclei scanned, in an average of four representative experiments). The x, y, and z coordinates were defined at the center of each hybridization signal and used to measure 3D distance by a mouse-driven cursor.
  • 17
    • 0024574230 scopus 로고
    • P. Emmerich et al , Exp Cell Res. 181, 126 (1989); J. P Lewis, H J. Tanke, A K Raap, G C. Beverstock, H. Klum-Nelemans, Hum Genet. 92, 577 (1993), L Manuelidis, Science 250, 1533 (1990).
    • (1989) Exp Cell Res. , vol.181 , pp. 126
    • Emmerich, P.1
  • 19
    • 0025649160 scopus 로고
    • P. Emmerich et al , Exp Cell Res. 181, 126 (1989); J. P Lewis, H J. Tanke, A K Raap, G C. Beverstock, H. Klum-Nelemans, Hum Genet. 92, 577 (1993), L Manuelidis, Science 250, 1533 (1990).
    • (1990) Science , vol.250 , pp. 1533
    • Manuelidis, L.1
  • 21
    • 13344290427 scopus 로고    scopus 로고
    • note
    • Mean nuclear diameter was 8 μm with a range of 6 to 12 μm, as determined by the 3D object count feature of Imagespace software No substantial differences in nuclear diameter were observed between different cell cycle fractions
  • 22
    • 13344252158 scopus 로고    scopus 로고
    • note
    • The distance of 2 0 μm was chosen on the basis of a comparison of the graph of late S phase lymphocytes to that of premeiotic yeast (22). The degree of nonrandomness was similar, but the scale of the graphs was different because the diameter of yeast nuclei was ∼5 μm, whereas lymphocytes are ∼6 to 12 μm (13). Pairing in premeiosis and meiosis may involve a tighter association between homologs, or yeast chromosomes may have fewer spatial constraints than chromosomes in higher eukaryotes, thus explaining the difference in actual distances.
  • 23
    • 13344256686 scopus 로고    scopus 로고
    • note
    • 2), lack of physical spreading of DNA by the altered FISH protocol, and larger probe see (centromeric repeats and 80- to 120-kb P1 clones) In the infrequent cases where replication doublets were observed, the measurement was taken at the point between the two signals Source numbers for P1 clones at Genomesystems are as follows: GABRB3/A5, 2269: D15S113, 6343; D13S104, 6269, D15S128, 7207; and D15S46 (ATCC)
  • 26
    • 0022392734 scopus 로고
    • S. A. Lesko, D E. Callahan, M. E. LaVilla, Z.-P. Wang, P, O P. Ts'o, ibid. 219, 499 (1995); L Manuelidis. Hum. Genet 71, 288 (1985)
    • (1985) Hum. Genet , vol.71 , pp. 288
    • Manuelidis, L.1
  • 27
    • 0029165883 scopus 로고
    • P. A. Leighton, J R. Saam, R. S Ingram, C. L. Stewart, S. M. Tilghman, Genes Dev 9, 2079 (1995), P. A. Leighton, R S Ingram, J. Eggenschwiler, A. Efstratiadis, S M Tilghman, Nature 375, 34 (1995); J S Sutcliffe et al., Nature Genet. 8, 52 (1994): K Buiting et al., ibid. 9, 395 (1995 )
    • (1995) Genes Dev , vol.9 , pp. 2079
    • Leighton, P.A.1    Saam, J.R.2    Ingram, R.S.3    Stewart, C.L.4    Tilghman, S.M.5
  • 28
    • 0029024277 scopus 로고
    • P. A. Leighton, J R. Saam, R. S Ingram, C. L. Stewart, S. M. Tilghman, Genes Dev 9, 2079 (1995), P. A. Leighton, R S Ingram, J. Eggenschwiler, A. Efstratiadis, S M Tilghman, Nature 375, 34 (1995); J S Sutcliffe et al., Nature Genet. 8, 52 (1994): K Buiting et al., ibid. 9, 395 (1995 )
    • (1995) Nature , vol.375 , pp. 34
    • Leighton, P.A.1    Ingram, R.S.2    Eggenschwiler, J.3    Efstratiadis, A.4    Tilghman, S.M.5
  • 29
    • 0028133293 scopus 로고
    • P. A. Leighton, J R. Saam, R. S Ingram, C. L. Stewart, S. M. Tilghman, Genes Dev 9, 2079 (1995), P. A. Leighton, R S Ingram, J. Eggenschwiler, A. Efstratiadis, S M Tilghman, Nature 375, 34 (1995); J S Sutcliffe et al., Nature Genet. 8, 52 (1994): K Buiting et al., ibid. 9, 395 (1995 )
    • (1994) Nature Genet. , vol.8 , pp. 52
    • Sutcliffe, J.S.1
  • 30
    • 0028939902 scopus 로고
    • P. A. Leighton, J R. Saam, R. S Ingram, C. L. Stewart, S. M. Tilghman, Genes Dev 9, 2079 (1995), P. A. Leighton, R S Ingram, J. Eggenschwiler, A. Efstratiadis, S M Tilghman, Nature 375, 34 (1995); J S Sutcliffe et al., Nature Genet. 8, 52 (1994): K Buiting et al., ibid. 9, 395 (1995 )
    • (1995) Nature Genet. , vol.9 , pp. 395
    • Buiting, K.1
  • 37
    • 13344266188 scopus 로고    scopus 로고
    • note
    • We thank W. Robinson, K Hirschom, S. Orkin, A. Bottani, B. Horsthemke, R Wharton, J Holden, B White, M. Higgins, A Flint, K. Glatt, O. M Liu, H. Lidov, J Wagstaff, and L M. Kunkel for their help and important contributions to this work Supported by Howard Hughes Medical Institute and NIH grant RO1-NS30628


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