Ethanol-related cytotoxicity catalyzed by CYP2E1-dependent generation of reactive oxygen intermediates in transduced HepG2 cells

BioFactors

Volumn 8, Issue 1-2, 1998, Pages 93-96

  Cederbaum, A I ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYUNSATURATED FATTY ACID; REACTIVE OXYGEN METABOLITE;

ANTIOXIDANT ACTIVITY; ANTIVIRAL ACTIVITY; APOPTOSIS; ARACHIDONIC ACID METABOLISM; CELL STRAIN HEPG2; CONFERENCE PAPER; CYTOTOXICITY; ENZYME ACTIVITY; LIPID PEROXIDATION; LIVER TOXICITY; PLASMID; PRIORITY JOURNAL; VIRUS INFECTION;

EID: 0031873850     PISSN: 09516433     EISSN: None     Source Type: Journal    
DOI: 10.1002/biof.5520080116     Document Type: Conference Paper

References (15)

1. 1 F.P. Guengerich, D.H. Kim and M. Iwasaki, Role of human cytochrome P450IIE1 in the oxidation of many low molecular weight cancer suspects, Chem. Res. Toxicol. 4 (1991), 168–179.
2. 2 D.R. Koop, Oxidative and reductive metabolism by cytochrome P4502E1, FASEB J. 6 (1992), 724–730.
3. 3 A.I. Cederbaum, Oxygen radical generation by microsomes: Role of iron and implications for alcohol metabolism and toxicity, Free Rad. Biol. Med. 7 (1989), 559–567.
4. 4 A.I. Cederbaum, Microsomal generation of reactive oxygen species and their possible role in alcohol hepatotoxicity, Alcohol Alcoholism 1 (Suppl.) (1991), 291–296.
5. 5 R. Nordmann, C. Ribiere and H. Rouach, Implications of free radical mechanisms in ethanol‐induced cellular injury, Free Rad. Biol. Med. 12 (1992), 219–240.
6. 6 M.U. Dianzani, Lipid peroxidation in ethanol poisoning: A critical reconsideration, Alcohol Alcoholism 20 (1985), 161–173.
7. 7 Y. Dai, J. Rashba‐Step and A.I. Cederbaum, Stable expression of human cytochrome P4502E1 in HepG2 cells: Characterization of catalytic activities and production of reactive oxygen intermediates, Biochemistry 32 (1993), 6928–6937.
8. 8 Y. Dai and A.I. Cederbaum, Cytotoxicity of acetaminophen in human cytochrome P4502E1‐transfected HepG2 cells, J. Pharmacol. Exp. Ther. 273 (1995), 1497–1505.
9. 9 Y. Dai and A.I. Cederbaum, Inactivation and degradation of human cytochrome P4502E1 by CCl4 in a transfected HepG2 cell line, J. Pharmacol. Exp. Ther. 275 (1995), 1614–1622.
10. 10 A. Carroccio, D. Wu and A.I. Cederbaum, Ethanol increases content and activity of human cytochrome P4502E1 in a transduced HepG2 cell line, Biochem. Biophys. Res. Comm. 203 (1994), 727–733.
11. 11 D. Wu and A.I. Cederbaum, Ethanol cytotoxicity to a transfected HepG2 cell line expressing human cytochrome P4502E1, J. Biol. Chem. 271 (1996), 23914–23919.
12. 12 S.W. French, Nutrition in the pathogenesis of alcoholic liver disease, Alcohol Alcoholism 28 (1993), 97–109.
13. 13 H. Tsukamoto, K. Gaal and S.W. French, Insights into the pathogenesis of alcoholic liver necrosis and fibrosis, Hepatology 12 (1990), 599–608.
14. 14 T. Hirano, N. Kaplowitz, H. Tsukamoto, S. Kamimura and J. Fernandez‐Checa, Hepatic mitochondrial glutathione depletion and progression of experimental alcoholic liver disease in rats, Hepatology 16 (1992), 1423–1427.
15. 15 Q. Chen, M. Galleano and A.I. Cederbaum, Cytotoxicity and apoptosis produced by arachidonic acid in HepG2 cells expressing human CYP2E1, J. Biol. Chem. 272 (1997), 14532–14541.