Construction of stable episomes in Cryptococcus neoformans

Current Genetics

Volumn 34, Issue 1, 1998, Pages 60-66

  Varma, Ashok ^a   Kwon Chung, K J ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

Author keywords

Cryptococcus neoformans; Plasmids; Transformation; Yeast

Indexed keywords

REPETITIVE DNA; URACIL;

ARTICLE; CRYPTOCOCCUS NEOFORMANS; DNA LIBRARY; DNA SEQUENCE; ELECTROPORATION; EPISOME; EXPRESSION VECTOR; MINICHROMOSOME; NONHUMAN; PLASMID; PRIORITY JOURNAL; TELOMERE;

BLOTTING, SOUTHERN; CHROMOSOMES, FUNGAL; CRYPTOCOCCUS NEOFORMANS; DEOXYRIBONUCLEASE ECORI; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA, FUNGAL; ELECTROPORATION; ESCHERICHIA COLI; MUTATION; PLASMIDS; POLYMERASE CHAIN REACTION; REPETITIVE SEQUENCES, NUCLEIC ACID; RESTRICTION MAPPING; TRANSFORMATION, GENETIC;

FILOBASIDIELLA NEOFORMANS; PROKARYOTA;

EID: 0031870620     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940050366     Document Type: Article

References (24)

1. Ascenzioni F, Della Valle G, Guerrini AM, Pisani G, Biondi O, Donini P (1990) A human DNA telomeric fragment contains yeast ARS and mitotic stabilizing sequences. Res Microbiol 141: 1117-1129
2. Chan CSM, Tye BK (1983) Organization of DNA sequences and replication origins at yeast telomeres. Cell 33:563-573
3. Chang YC, Kwon-Chung KJ (1994) Complementation of a capsule-deficient mutant of Cryptococcus neoformans restores its virulence. Mol Cell Biol 14:4912-4919
4. Chang YC, Penoyer L, Kwon-Chung KJ (1996) The second capsule gene of Cryptococcus neoformans. CAP64, is essential for virulence. Infect Immun 64:1977-1983
5. Cox GM, Toffaletti DL, Perfect JR (1996) Dominant selection system for use in Cryptococcus neoformans. J Med Vet Mycol 34: 385-391
6. Edman JC (1992) Isolation of telomere-like sequences from Cryptococcus neoformans and their use in high-efficiency transformation. Mol Cell Genet 12:2777-2783
7. Edman JC, Kwon-Chung KJ (1990) Isolation of the ura5 gene from Cryptococcus neoformans var neoformans and its use as a selectable marker for transformation. Mol Cell Biol 10:4538-4544
8. Fincham JRS (1989) Transformation of fungi. Microbiol Rev 53: 148-170
9. Fitzgerald-Hayes M (1987) Yeast centromeres Yeast 3: 187-200
10. Gilley D, Preer JR, Aufderheide KJ, Polisky P (1988) Autonomous replication and addition of telomere-like sequences to DNA microinjected into Paramecium tetraurelia macronuclei. Mol Cell Biol 8:4765-4772
11. Kwon-Chung KJ, Bennett JE (1992) Cryptococcosis. In: Medical mycology. Lea and Febiger, Malvern, Pennsylvania, pp 397-446
12. Odom A, Muir S, Lim E, Toffaletti DL, Perfect J, Heitman J (1997) Calcineurin is required for virulence in Cryptococcus neoformans. EMBO J 16:2576-2589
13. Powell WA, Kistler HC (1990) In vivo rearrangement of foreign DNA by Fusarium oxysporum produces linear self-replicating plasmids. J Bacteriol 172:3163-3171
14. Rodriguez RJ (1993) Polyphosphate present in DNA preparations from filamentous fungal species of Colletotrichum inhibits restriction endonucleases and other enzymes. Anal Biochem 209: 291-297
15. Stahl LL, Akins RA, Lambowitz AL (1984) Characterization of deletion derivatives of an autonomously replicating Neurospora plasmid. Nucleic Acids Res 12:6169-6178
16. Toffaletti DL, Rude T, Johnston SA, Durack DT, Perfect JR (1993) Gene transfer in Cryptococcus neoformans by use of biolistic delivery of DNA. J Bacteriol 175:1405-1411
17. Varma A, Kwon-Chung KJ (1991) Rapid method to extract DNA from Cryptococcus neoformans. J Clin Microbiol 29:810-812
18. Varma A, Kwon-Chung KJ (1992) Molecular and genetic analysis of URA5 transformants of Cryptococcus neoformans. Infect Immun 60: 1101-1108
19. Varma A, Kwon-Chung KJ (1994) Generation of a minichromosome in Cryptococcus neoformans Curr Genet 26: 54-51
20. Varma A, Kwon-Chung KJ (1995) Diversity of DNA fingerprints in Cryptococcus neoformans. J Clin Microbiol 33: 1807-1814
21. Walmsley RM (1987) Yeast telomeres: the end of the chromosome story. Yeast 3: 139-148
22. Williamson PR (1994) Biochemical and molecular characterization of the diphenol oxidase of Cryptococcus neoformans: identification as a laccase. J Bacteriol 176:656-664
23. Woods JP, Goldman WE (1993) Autonomous replication of foreign DNA in Histoplasma capsulatum: the role of native telomeric sequences. J Bacteriol 175:636-641
24. Wright APH, Mandrell K, Shull S (1986) Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genome Curr Genet 10:503-508