Topological analysis of the Rhodobacter capsulatus PucC protein and effects of C-terminal deletions on light-harvesting complex II

Journal of Bacteriology

Volumn 178, Issue 16, 1996, Pages 4801-4806

  Leblanc, H N ^a,b   Beatty, J T ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BACTERIAL PROTEIN; BETA GALACTOSIDASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE DELETION; GENE FUSION; NONHUMAN; PHOTOSYNTHESIS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RHODOBACTER CAPSULATUS;

BACTERIA (MICROORGANISMS); RHODOBACTER CAPSULATUS; RHODOPSEUDOMONAS;

EID: 0029742631     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.16.4801-4806.1996     Document Type: Article

References (36)

1. Adams, C. W., M. E. Forrest, S. N. Cohen, and J. T. Beatty. 1989. Structural and functional analysis of transcriptional control of The Rhodobacter capsulatus puf operon. J. Bacteriol. 171:473-482.
2. Bauer, C. E., J. Buggy, Z. Yang, and B. L. Marrs. 1991. The superoperonal organization of genes for pigment biosynthesis and reaction center proteins is a conserved feature in R. capsulatus: analysis of overlapping bchB and puhA transcripts. Mol. Gen. Genet. 228:438-444.
3. Beatty, J. T., and H. Gest. 1981. Generation of succinyl-coenzyme A in photosynthetic bacteria. Arch. Microbiol. 129:335-340.
4. Bibb, M. J., and S. N. Cohen. 1982. Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol. Gen. Genet. 187:265-277.
5. Bingle, W., H. D. Kurtz, and J. Smit. 1993. An "all-purpose" cellulase reporter for gene fusion studies and applications to the paracrystalline surface (S)-layer protein of Caulobacter crescentus. Can. J. Microbiol. 39:70-80.
6. Bollivar, D. W., J. Y. Suzuki, J. T. Beatty, J. M. Dobrowolski, and C. E. Bauer. 1994. Directed mutalional analysis of bacteriochlorophyll a biosynthesis in Rhodobacter capsulatus. J. Mol. Biol. 237:622-640.
7. Boyd, D., and J. Beckwith. 1989. Positively charged amino acid residues can act as topogenic determinants of membrane proteins. Proc. Natl. Acad. Sci. USA 86:9446-9450.
8. Boyd, D., B. Traxler, and J. Beckwith. 1993. Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions. J. Bacteriol. 175:553-556.
9. Davison, J., M. Heusterspreute, N. Chevalier, V. Ha-Thi, and F. Brunel. 1987. Vectors with restriction site banks. V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51:275-280.
10. Drews, G., and J. R. Golecki. 1995. Structure, molecular organization, and biosynthesis of membranes of purple bacteria, p. 231-257. In R. E. Blankenship, M. T. Madigan, and C. E. Bauer (ed.), Anoxygenic photosynthetic bacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
11. Eisenberg, D., E. Schwarz, M. Komaromy, and R. Wall. 1984. Analysis of membrane and surface protein sequences with the hydrophobic moment plot. J. Mol. Biol. 179:125-142.
12. Farchaus, J. W., W. P. Ban, H. Grünberg, and D. Oesterhelt. 1992. Studies on the expression of the pufX polypeptide and its requirement for photoheterotrophic growth in Rhodobacter sphaeroides. EMBO J. 11:2779-2788.
13. 12a.Finlay, B. Personal communication.
14. Gafvelin, G., and G. von Heijne. 1994. Topological "frustration" in multispanning E. coli inner membrane proteins. Cell 77:401-412.
15. Green, B. R. 1990. Structure prediction methods for membrane proteins: comparison with the X-ray structure of the R. viridis photosynthetic reaction center, p. 395-404. In J. J. Villafranca (ed.), Current research in protein chemistry. Academic Press, New York.
16. Karrasch, S., P. A. Bullough, and R. Ghosh. 1995. The 8.5 Å projection map of the light-harvesting complex I from Rhodospirillum rubrum reveals a ring composed of 16 subunits. EMBO J. 14:631-638.
17. Klein, P., M. Kanehisa, and C. DeLisi. 1985. The detection and classification of membrane-spanning proteins. Biochim. Biophys. Acta 815:468-476.
18. Lancaster, C. R. D., U. Ermler, and H. Michel. 1995. The structures of photosynthetic reaction centers from purple bacteria as revealed by X-ray crystallography, p. 503-526. In R. E. Blankenship, M. T. Madigan, and C. E. Bauer (ed.), Anoxygenic photosynthetic bacteria. Kluwer Academic Publishers, Dordrecht, The Netherlands.
19. LeBlanc, H. N. 1995. Directed mutagenesis and gene fusion analysis of the Rhodobacter capsulatus puc operon. Ph.D. thesis. The University of British Columbia, Vancouver, British Columbia, Canada.
20. 18a.LeBlanc, H. N., and J. T. Beatty. 1993. Rhodobacter capsulatus puc operon: promoter location, transcript sizes and effects of deletions on photosynthetic growth. J. Gen. Microbiol. 139:101-109.
21. Lilburn, T. G., R. C. Prince, and J. T. Beatty. 1995. Mutation of the Ser2 codon of the light-harvesting B870 α polypeptide of Rhodobacter capsulatus partially suppresses the pufX phenotype. J. Bacteriol. 177:4593-4600.
22. Manoil, C. 1991. Analysis of membrane protein topology using alkaline phosphatase and β-galactosidase. Methods Cell Biol. 34:61-75.
23. Manoil, C., and J. Beckwith. 1986. A genetic approach to analyzing membrane protein topology. Science 233:1403-1408.
24. McDermott, G., S. M. Prince, A. A. Freer, A. M. Hawthornthwaite-Lawless, M. Z. Papiz, R. J. Cogdell, and N. W. Isaacs. 1995. Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature (London) 374:517-521.
25. Miller, S., J. Janin, A. M. Lesk, and C. Chothia. 1987. Interior and surface of monomeric proteins. J. Mol. Biol. 196:641-656.
26. Rao, J. K. M., and P. Argus. 1986. A conformational preference parameter to predict helices in integral membrane proteins. Biochim. Biophys. Acta 869:197-217.
27. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. San Millan, J. L., D. Boyd, R. Dalbey, W. Wickner, and J. Beckwith. 1989. Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase. J. Bacteriol. 171:5536-5541.
29. 26a.Suzuki, J. Personal communication.
30. Tadros, M. H., A. F. Garcia, G. Drews, N. Gad'on, and M. P. Skatchkov. 1990. Isolation and characterization of a light harvesting complex II lacking the γ-polypeptide from Rhodobacter capsulatus. Biochim. Biophys. Acta 1019:245-249.
31. Tichy, H. V., K. U. Albien, N. Gad'on, and G. Drews. 1991. Analysis of the Rhodobacter capsulatus puc operon - the pucC gene plays a central role in the regulation of LHII (B800-850 complex) expression. EMBO J. 10:2949-2955.
32. Tichy, H. V., B. Oberlé, H. Stiehle, E. Schiltz, and G. Drews. 1989. Genes downstream from pucB and pucA are essential for formation of the B800-850 complex of Rhodobacter capsulatus. J. Bacteriol. 171:4914-4922.
33. von Heijne, G. 1994. Membrane proteins: from sequence to structure. Annu. Rev. Biophys. Biomol. Struct. 23:167-192.
34. Wong, D. K.-H., W. J. Collins, A. Harmer, T. G. Lilburn, and J. T. Beatty. 1996. Directed mutagenesis of the Rhodobacter capsulatus puhA gene and Orf214: pleiotropic effects on photosynthetic reaction center and light-harvesting 1 complexes. J. Bacteriol. 178:2334-2342.
35. 31a.Young, C., and J. T. Beatty. Unpublished data.
36. Youvan, D. C., and S. Ismail. 1985. Light harvesting II (B800-850 complex) structural genes from Rhodopseudomonas capsulata. Proc. Natl. Acad. Sci. USA 82:58-62.