Short oligonucleotides as external guide sequences for site-specific cleavage of RNA molecules with human RNase P

RNA

Volumn 4, Issue 7, 1998, Pages 847-855

  Werner, Martina ^a   Rosa, Eddie ^a   Nordstrom, Jeffrey L ^a,b   Goldberg, Allan R ^a   George, Shaji T ^a  

^a Innovir Laboratories Inc   (United States)

^b Gene Medicine Inc   (United States)

Author keywords

Enzyme substrate recognition; HBV viral RNA; RNA motif; T loop structure; Targeting

Indexed keywords

RIBONUCLEASE P; TRANSFER RNA; VIRUS RNA;

ARTICLE; ENZYME PURIFICATION; ENZYME SUBSTRATE; ESCHERICHIA COLI; HELA CELL; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; RNA SEQUENCE; RNA SYNTHESIS;

BASE SEQUENCE; ENDORIBONUCLEASES; HEPATITIS B SURFACE ANTIGENS; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; OLIGORIBONUCLEOTIDES; RIBONUCLEASE P; RNA PRECURSORS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, CATALYTIC; RNA, GUIDE; RNA, TRANSFER; RNA, VIRAL; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; HEPATITIS B VIRUS; RNA VIRUSES;

EID: 0031848340     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838298980323     Document Type: Article

References (26)

1. Abramovitz DL, Pyle AM. 1997. Remarkable morphological variability of a common RNA folding motif: The GNRA tetraloop-receptor interaction. J Mol Biol 266:493-506.
2. Altman S. 1995. RNase P in research and therapy. Bio/Technology 13:327-329.
3. Bartkiewicz M, Gold H, Altman S. 1989. Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells. Genes & Dev 3:488-499.
4. Carrara G, Calandra P, Fruscolini P, Doria M, Tocchini-Valentini GP. 1989. Site selection by Xenopus laevis RNase P. Cell 58:37-45.
5. Carrara G, Calandra P, Fruscolini P, Tocchini-Valentini GP. 1995. Two helices plus a linker: A small model substrate for eukaryotic RNase P. Proc Natl Acad Sci USA 92:2627-2631.
6. Conrad F, Hanne A, Gaur RK, Krupp G. 1995. Enzymatic synthesis of 2′ modified nucleic acids: Identification of important phosphates and ribose moieties in RNase P substrates. Nucleic Acids Res 23:1845-1853.
7. Cummins LL, Owens SR, Risen LM, Lesnik EA, Freier SM, McGee D, Guinosso CJ, Cook PD. 1995. Characterization of fully 2′-modified. oligoribonucleotide hetero- and homoduplex hybridization and nuclease sensitivity. Nucleic Acids Res 23:2019-2024.
8. Foṙster AC, Altman S. 1990. External guide sequences for an RNA enzyme. Science 249:783-786.
9. Gaur RK, Krupp G. 1993. Modification interference approach to detect ribose moieties important for the optimal activity of a ribozyme. Nucleic Acids Res 21:21-26.
10. Hardt WD, Schlegl J, Erdmann VA, Hartmann RK. 1993. Role of the D arm and anticodon stem in tRNA recognition by eubacterial and eukaryotic RNase P enzyme. Biochemistry 32:13046-13053.
11. Hartmann RK, Krupp G, Hardt WD. 1995. Towards a new concept of gene inactivation: Specific RNA cleavage by ribonuclease P. In: El-Gewely MR, ed. Biotechnology Annual Review 1. New York: Elsevier Science, pp 215-265.
12. Jaeger JA, Turner DH, Zuker M. 1989. Improved predictions of secondary structure for RNA. Proc Natl Acad Sci USA 86:7706-7710.
13. Kahle D, Wehmeyer U, Krupp G. 1990. Substrate recognition by RNase P and by the catalytic M1 RNA: Identification of possible contact points in pre-tRNAs. EMBO J 9:1929-1937.
14. Krupp G. 1993. Antisense oligoribonucleotides and RNase P. A great potential. Biochimie 75:135-138.
15. Loria A, Pan T. 1997. Recognition of the T stem-loop of a pre-tRNA substrate by the ribozyme from Bacillus subtilis ribonuclease P. Biochemistry:6317-6325.
16. Mathews DH, Andre TC, Kim J, Turner DH, Zuker M. 1998. An updated recursive algorithm for RNA secondary structure. In: Leontis NB, SantaLucia J, eds. Molecular modeling of nucleic acids. ACS Symposium Series 682. Forthcoming.
17. Milligan JF, Groebe DR, Witherell GW, Uhlenbeck OC. 1987. Oligonucleotide synthesis using T7 polymerase and synthetic DNA templates. Nucleic Acids Res 15:8783-8798.
18. Pan T, Gutell RR, Uhlenbeck OC. 1991. Folding of circularly permuted transfer RNAs. Science 254:1361-1364.
19. Pan T, Loria A, Zhong K. 1995. Probing of tertiary interactions in RNA: 2′-Hydroxyl-base contacts between the RNase P RNA and pre-tRNA. Proc Natl Acad Sci USA 92:12510-12514.
20. Scaringe SA, Francklyn C, Usman N. 1990. Chemical synthesis of biologically active oligoribonucleotides using β-cyanoethyl protected ribonucleoside phosphoramidites. Nucleic Acids Res 18: 5433-5441.
21. Thurlow LT, Shilowski D, Marsh TL 1991. Nucleotides in precursor tRNA that are required intact for catalysis by RNase P RNAs. Nucleic Acids Res 19:885-890.
22. Walter AE, Turner DH, Kim J, Lyttle MH, Muller P, Mathews DH, Zuker M. 1994. Coaxial stacking of helixes enhances binding of oligoribonucleotides and improves predictions of RNA folding. Proc Natl Acad Sci USA 91:9218-9222.
23. Werner M, Rosa E, Goldberg AR, George ST. 1998. Targeted cleavage of RNA molecules by human RNase P using minimized external guide sequences. Manuscript in preparation.
24. Yuan Y, Altman S. 1995. Substrate recognition by human RNase P: Identification of small model substrates for the enzyme. EMBO J 14:159-168.
25. Yuan Y, Hwang ES, Altman S. 1992. Targeted cleavage of mRNA by human RNase P. Proc Natl Acad Sci USA 89:8006-8010.
26. Zuker M. 1989. On finding all suboptimal foldings of an RNA molecule. Science 244:48-52.