Analysis of mRNA from microdissected frozen tissue sections without RNA isolation

American Journal of Pathology

Volumn 153, Issue 1, 1998, Pages 47-51

  To, Minh D ^a,c   Done, Susan J ^b,c   Redston, Mark ^b,c   Andrulis, Irene L ^a,b,c  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c MOUNT SINAI HOSPITAL   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 2 MICROGLOBULIN; PROTEIN P21;

ARTICLE; CELL ONCOGENE; CELL TRANSFORMATION; CONTROLLED STUDY; DNA DETERMINATION; GENE EXPRESSION; HUMAN; HUMAN CELL; MOLECULAR CLONING; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOLID TUMOR; TECHNIQUE; TUMOR CELL;

EID: 0031847204     PISSN: 00029440     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0002-9440(10)65544-7     Document Type: Article

References (6)

1. Zhuang Z, Bertheau P, Emmert-Buck MR, Liotta LA, Gnarra J, Linenhan WM, Lubensky IA: A microdissection technique for archival DNA analysis of specific cell populations in lesions <1 mm in size. Am J Pathol 1995, 146:620-625
2. Moskaluk CA, Kern SE: Microdissection and polymerase chain reaction amplification of genomic DNA from histological tissue sections. Am J Pathol 1997, 150:1547-1552
3. Miller T, Snell L, Watson PH: Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections. Biotechniques 1996, 21:38-43
4. Houze TA, Gustavsson B: Sonification as a means of enhancing the detection of gene expression levels from formalin-fixed, paraffin-embedded biopsies. Biotechniques 1996, 21:1074-1082
5. Leygue ER, Watson PH, Murphy LC: Identification of differential expressed genes using minute amounts of RNA. Biotechniques 1996, 21:1008-1012
6. Klebe RJ, Grant GM, Grant AM, Garcia MA, Giambernardi TA, Taylor GP: RT-PCR without RNA isolation. Biotechniques 1996, 21:1094-1100