Sonification as a means of enhancing the detection of gene expression levels from formalin-fixed, paraffin-embedded biopsies

BioTechniques

Volumn 21, Issue 6, 1996, Pages 1074-1082

  Houze, Thomas A ^a   Gustavsson, Bengt ^a  

^a SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

BETA ACTIN; FORMALDEHYDE; PARAFFIN;

ANALYTIC METHOD; ARTICLE; BIOPSY; EMBEDDING; EXTRACTION; FROZEN SECTION; GENE EXPRESSION; HUMAN; HUMAN TISSUE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ULTRASOUND;

EID: 0029730688     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96216st05     Document Type: Article

References (12)

1. Bouaboula, M., P. Legoux, B. Pességué, B. Delpech, X. Dumont, M. Piechaczyk, P. Casellas and D. Shire. 1992. Standardization of mRNA titration using a PCR method involving co-amplification with a multispecific internal control. J. Biol. Chem. 267:21830-21838.
2. Chelly, J., J.C. Kaplan, P. Maire, S. Gautron and A. Kahn. 1988. Transcription of the dystrophin gene in human muscle and non-muscle tissue. Nature 333:858-860.
3. de Andrés, B., V. del Pozo, S. Gallardo, E. de Arruda-Chaves, B. Cárdaba, E. Martín-Orozco, M. Posada, P. Palomino and C. Lahoz. 1995. Improved method for mRNA extraction from paraffin-embedded tissue. BioTechniques 18:42-44.
4. Delidow, B.C., J.J Peluso and B.A. White. 1989. Re-evaluation of the effects of growth hormone and prolactin on tadpole growth and development. Genet. Anal. Tech. Appl. 6:120-124.
5. Horikoshi, T., K.D. Danenberg, T.H.W. Stadbauer, M. Volkenandt, L.C.C. Shea, K. Aigner, B. Gustavsson, L. Leichman et al. 1992. Quantitation of thymidylate synthase, dihydrofolate reductase, and DT-diaphorase gene expression in human tumors using the polymerase chain reaction. Cancer Res. 52:108-116.
6. Horikoshi, T., K. Danenberg, M. Volkenandt, T. Stadbauer and P.V. Danenberg. 1993. Quantitative measurement of relative gene expression in human tumors, p. 177-187. In B. White (Ed.), Current Methods and Applications. Humana Press, Ottawa.
7. Houze, T.A., L. Larsson, P.A. Larsson, G. Hansson, A. Asea and B. Gustavsson. 1996. Rapid detection of thymidylate synthase gene expression levels by semi-quantitative competitive reverse transcriptase polymerase chain reaction followed by quantitative digital image analysis. Tumor Biol. 17:306-319.
8. Montgomery, R.A. and M. Dallman. 1991. Analysis of cytokine gene expression during fetal thymic ontogeny using the polymerase chain reaction. J. Immunol. 147:554-560.
9. Murray, L.J., R. Lee and C. Martens. 1990. In vivo cytokine gene expression in T-cell subsets of the autoimmune MRL/Mp-Ipr mouse. Eur. J. Immunol. 20:163-170.
10. Rappolee, D.A., A. Wang, D. Mark and Z.J. Werb. 1989. Novel method for studying mRNA phenotypes in single and small numbers of cells. Cell Biochem. 39:71-81.
11. Saiki, R.K., S. Scharf, F. Falona, K.B. Mullis., G.T. Horn, H. Erlich and N. Arnheim. 1985. Enzymatic amplification of β-globin genomic sequences and resolution site analysis for diagnosis of sickle cell anemia. Science 230:1350-1353.
12. Watkins, D.I., S.N. McAdam, X. Liu, C.R. Strang, E.L. Milford, C.G. Levine and T.L. Garber. 1992. New recombinant HLA-B alleles in a tribe of South American Amerindians indicate rapid evolution of MHC class I loci. Nature 357:329-333.