Cloning and characterization of a prolinase gene (pepR) from Lactobacillus rhamnosus

Applied and Environmental Microbiology

Volumn 64, Issue 5, 1998, Pages 1831-1836

  Varmanen, Pekka ^a   Rantanen, Terhi ^b   Palva, Airi ^b   Tynkkynen, Soile ^a  

^a VALIO LTD   (Finland)

^b FINNISH FOREST RESEARCH INSTITUTE   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; ENZYME ACTIVITY; ENZYME STRUCTURE; GENE EXPRESSION; GENE MUTATION; GENE STRUCTURE; LACTOBACILLUS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION;

LACTOBACILLUS HELVETICUS; LACTOBACILLUS RHAMNOSUS; NEGIBACTERIA; PROKARYOTA;

EID: 0031834280     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.5.1831-1836.1998     Document Type: Article

References (46)

1. Anderson, D. G., and L. L. McKay. 1983. Simple and rapid method for isolating large plasmid DNA from lactic streptococci. Appl. Environ. Microbiol. 46:549-552.
2. Arora, G., and B. H. Lee. 1992. Purification and characterization of aminopeptidase from Lactobacillus casei subsp. casei LLG. J. Dairy Sci. 75:700-710.
3. Arora, G., and B. H. Lee. 1994. Purification and characterization of an aminopeptidase from Lactobacillus casei subsp. rhamnosus S93. Biotechnol. Appl. Biochem. 19:179-192.
4. Atlan, D., C. Gilbert, B. Blanc, and R. Portalier. 1994. Cloning, sequencing and characterization of the pepIP gene encoding a proline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397. Microbiology 140:527-535.
5. Aymerich, M. T., M. Hugas, M. Garriga, R. F. Vogel, and J. M. Monfort. 1993. Electrotransformation of meat lactobacilli. Effect of several parameters on their efficiency of transformation. J. Appl. Bacteriol. 75:320-325.
6. Baankreis, R., and F. Exterkate. 1991. Characterization of a peptidase from Lactococcus lactis ssp. cremoris HP that hydrolyses di-and tripeptides containing proline or hydrophobic residues as the aminoterminal amino acid. Syst. Appl. Microbiol. 14:317-323.
7. Bhowmik, T., L. Fernández, and J. L. Steele. 1993. Gene replacement in Lactobacillus helveticus. J. Bacteriol. 175:6341-6344.
8. Bromme, M. C., D. A. Krause, and M. W. Hickey. 1990. The isolation and characterization of lactobacilli from cheddar cheese. Aust. J. Dairy Technol. 45:60-66.
9. Christensen, J. E., and J. L. Steele. 1996. Characterization of peptidase-deficient Lactobacillus helveticus CNRZ32 derivatives, abstr. K24. In Abstracts of the Fifth Symposium on Lactic Acid Bacteria. Federation of European Microbiological Societies, Publications Office, Glasgow, United Kingdom.
10. Doi, E., D. Shibata, and T. Matoba. 1981. Modified colorimetric ninhydrin methods for peptidase assay. Anal. Biochem. 118:173-184.
11. Dudley, E. G., A. C. Husgen, W. He, and J. L. Steele. 1996. Sequencing, distribution, and inactivation of the dipeptidase A gene (pepDA) from Lactobacillus helveticus CNRZ32. J. Bacteriol. 178:701-704.
12. Dudley, E. G., and J. L. Steele. 1994. Nucleotide sequence and distribution of the pepPN gene from Lactobacillus helveticus CNRZ32. FEMS Microbiol. Lett. 119:41-46.
13. El Abboudi, M., M. El Soda, S. Pandian, R. E. Simard, and N. F. Olson. 1992. Purification of X-prolyl dipeptidyl aminopeptidase from Lactobacillus casei subspecies. Int. J. Food Microbiol. 15:87-98.
14. Fernández-Esplá, M. D., and M. C. Martín-Hernández. 1997. Purification and characterization of a dipeptidase from Lactobacillus casei ssp. casei IFPL 731 isolated from goat cheese made from raw milk. J. Dairy Sci. 80:1497-1504.
15. Fernández-Esplá, M. D., M. C. Martín-Hernández, and P. F. Fox. 1997. Purification and characterization of a prolidase from Lactobacillus casei subsp. casei IFPL 731. Appl. Environ. Microbiol. 63:314-316.
16. Fox, P. 1989. Proteolysis during cheese manufacture and ripening. J. Dairy Sci. 72:1379-1400.
17. Gasson, M. J., and G. F. Fitzgerald. 1994. Gene transfer systems and transposition, p. 1-51. In M. Gasson and W. M. De Vos (ed.). Genetics and biotechnology of lactic acid bacteria. Blackie Academic and Professional. London, England.
18. Gilbert, C., D. Atlan, B. Blanc, and R. Portalier. 1994. Proline iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus CNRZ 397: purification and characterization. Microbiology 140:537-542.
19. Habibi-Najafi, M. B., and B. H. Lee. 1994. Purification and characterization of X-prolyl dipeptidyl peptidase from Lactobacillus casei subsp. casei LLG. Appl. Microbiol. Biotechnol. 42:280-286.
20. Habibi-Najafi, M. B., and B. H. Lee. 1995. Purification and characterization of proline iminopeptidase from Lactobacillus casei subsp. casei LLG. J. Dairy Sci. 78:251-259.
21. Hames, B., and S. Higgins. 1985. Nucleic acid hybridization: a practical approach. IRL Press, Oxford, United Kingdom.
22. Hellendoorn, M. A., B. M. D. Franke-Fayard, I. Mierau, G. Venema, and J. Kok. 1997. Cloning and analysis of the pepV dipeptidase gene of Lactococcus lactis MG1363. J. Bacteriol. 179:3410-3415.
23. Holck, A., and H. Naes. 1992. Cloning, sequencing and expression of the gene encoding the cell-envelope-associated proteinase from Lactobacillus paracasei subsp. paracasei NCDO151. J. Gen. Microbiol. 138:1353-1364.
24. Klein, J. R., U. Klein, M. Schad, and R. Plapp. 1993. Cloning, DNA sequence analysis and partial characterization of pepN, a lysyl aminopeptidase from Lactobacillus delbrueckii subsp. lactis DSM7290. Eur. J. Biochem. 217: 105-114.
25. Klein, J. R., U. Schmidt, and R. Plapp. 1994. Cloning, heterologous expression, and sequencing of a novel proline iminopeptidase gene, pepI, from Lactobacillus delbrueckii subsp. lactis DSM7290. Microbiology 140:1133-1139.
26. Klein, J. R., J. Schick, B. Henrich, and R. Plapp. 1997. Lactobacillus delbrueckii subsp. lactis DSM7290 pepG gene encodes a novel cysteine aminopeptidase. Microbiology 143:527-537.
27. Kok, J., and W. M. De Vos. 1994. The proteolytic system of lactic acid bacteria, p. 169-210. In M. Gasson and W. M. De Vos (ed.), Genetics and biotechnology of lactic acid bacteria. Blackie Academic and Professional, London, England.
28. Kunji, E. R. S., I. Mierau, A. Hagting, B. Poolman, and W. N. Konings. 1996. The proteolytic system of lactic bacteria. Antonie Leeuwenhoek 70:187-221.
29. Kyte, J., and R. F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
30. Miller, C. G., and K. Mackinnon. 1974. Peptidase mutants of Salmonella typhimurium. J. Bacteriol. 120:355-363.
31. Miller, C. G., and G. Schwartz. 1978. Peptidase-deficient mutants of Escherichia coli. J. Bacteriol. 135:603-611.
32. Myöhänen, S., and J. Wahlfors. 1993. Automated fluorescent primer extension. BioTechniques 14:16-17.
33. Palva, A., K. Nyberg, and I. Palva. 1988. Quantitation of α-amylase mRNA in Bacillus subtilis by nucleic acid sandwich hybridization. DNA 7:135-142.
34. Peterson, S. D., and R. T. Marshall. 1990. Nonstarter lactobacilli in cheddar cheese: a review. J. Dairy Sci. 73:1395-1410.
35. Rawlings, N., L. Polgar, and A. Barret. 1991. A new family of serine type peptidases related to prolyl oligopeptidase. Biochem. J. 279:907-911.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
37. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
38. Shao, W., G. Ü. Yüksel, E. G. Dudley, K. L. Parkin, and J. L. Steele. 1997. Biochemical and molecular characterization of PepR, a dipeptidase, from Lactobacillus helveticus CNRZ32. Appl. Environ. Microbiol. 63:3438-3443.
39. Simon, D., and A. Chopin. 1988. Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis. Bichimie 70:559-567.
40. Tilsala-Timisjärvi, A., and T. Alatossava. 1997. Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR. Int. J. Food Microbiol. 35:49-56.
41. Troll, W., and J. Lindsley. 1955. A photometric method for the determination of proline. J. Biol. Chem. 215:655-661.
42. Varmanen, P., T. Rantanen, and A. Palva. 1996. An operon from Lactobacillus helveticus composed of a proline iminopeptidase gene (pepI) and two genes coding for putative members of the ABC transporter family of proteins. Microbiology 142:3459-3468.
43. Varmanen, P., J. Steele, and A. Palva. 1996. Characterization of prolinase gene and its product and an adjacent ABC transporter gene from Lactobacillus helveticus. Microbiology 142:809-816.
44. Vesanto, E., K. Savijoki, T. Rantanen, J. L. Steele, and A. Palva. 1995. An X-prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus. Microbiology 141:3067-3075.
45. Vesanto, E., P. Varmanen, J. L. Steele, and A. Palva. 1994. Characterization and expression of the Lactobacillus helveticus pepC gene encoding a general aminopeptidase. Eur. J. Biochem. 224:991-997.
46. Yüksel, G. Ü., and J. L. Steele. 1996. DNA sequence analysis, expression, distribution and the physiological role of the Xaa-prolyldipeptidyl aminopeptidase gene from Lactobacillus helveticus CNRZ32. Appl. Microbiol. Biotechnol. 44:766-773.