Lactobacillus delbrueckii subsp. lactis DSM7290 pepG gene encodes a novel cysteine aminopeptidase

Microbiology

Volumn 143, Issue 2, 1997, Pages 527-537

  Klein, Jürgen R ^a   Schick, Joachim ^a   Henrich, Bernhard ^a   Plapp, Roland ^a  

^a Rheinland Pfälzische Technische Universität Kaiserslautern Landau   (Germany)

Author keywords

Cysteine aminopeptidases; Gene duplication; Lactobacillus delbrueckii subsp. lactis

Indexed keywords

CYSTYL AMINOPEPTIDASE;

ARTICLE; COMPARATIVE STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA; FERMENTATION; GENE; GENE LIBRARY; GENETICS; LACTIC ACID BACTERIUM; LACTOBACILLUS DELBRUECKII; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; RECOMBINANT PLASMID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LACTOBACILLUS; LACTOBACILLUS DELBRUECKII SUBSP. LACTIS; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0031047672     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-143-2-527     Document Type: Article

References (33)

1. Carter, T. & Miller, C. G. (1984). Aspartate specific peptidases in Salmonella typhimurium: mutants deficient in peptidase E. J Bacteriol 159, 453-459.
2. Chapot-Chartier, M. P., Nardi, M., Chopin, M. C., Chopin, A. & Gripon, J. C. (1993). Cloning of pepC, a cysteine aminopeptidase from Lactococcus lactis subsp. cremoris AM2. Appl Environ Microbiol 59, 330-333.
3. Chapot-Chartier, M. P., Rul, F., Nardi, M. & Gripon, J. C. (1994). Gene cloning and characterization of PepC, a cysteine aminopeptidase from Streptococcus thermophilus, with sequence similarity to the eucaryotic bleomycin hydrolase. Eur J Biochem 224, 497-506.
4. Christensen, J. E., Lin, V., Palva, V. & Steele, J. L. (1995). Sequence analysis, distribution and expression of an aminopeptidase N-encoding gene from Lactobacillus helveticus CNRZ32. Gene 164, 189-190.
5. De Man, J. C., Rogosa, M. & Sharpe, M. E. (1960). A medium for the cultivation of Lactobacilli. J Appl Bacteriol 23, 130-135.
6. Dower, W. J., Miller, J. F. & Ragsdale, C. W. (1988). High efficiency transformation of E. coli by high voltage electroporation. Nucleic Acids Res 16, 2127-2145.
7. Dudley, E. G., Husgen, A. C., He, W. & Steele, J. L. (1996). Sequencing, distribution, and inactivation of the dipeptidase A gene (pepDA) from Lactobacillus helveticus CNRZ32. J Bacteriol 178, 701-704.
8. Fernández, L., Bhowmik, T. & Steele, J. L. (1994). Characterization of the Lactobacillus helveticus CNRZ32 pepC gene. Appl Environ Microbiol 60, 333-336.
9. Gilbert, C., Atlan, D., Blanc, B., Portalier, R., Germond, J. E. G., Lapierre, L. & Mollet, B. (1996). A new cell surface proteinase : sequencing and analysis of the prtB gene from Lactobacillus delbrueckii subsp. bulgaricus. J Bacteriol 178, 3059-3065.
10. Kamphuis, I. G., Drenth, J. & Baker, E. N. (1985). Thiol proteases. Comparative studies based on the high-resolution structures of papain and actinidin, and on amino acid sequence information for cathepsins B and H, and stem bromelain. J Mol Biol 182, 317-329.
11. Klein, J. R., Klein, U., Schad, M. & Plapp, R. (1993). Cloning, DNA sequence analysis and partial characterization of pepN, a lysyl aminopeptidase from Lactobacillus delbrueckii subsp. lactis DSM7290. Eur J Biochem 217, 105-114.
12. Klein, J. R., Henrich, B. & Plapp, R. (1994). Cloning and nucleotide sequence analysis of the Lactobacillus delbrueckii subsp. lactis DSM7290 cysteine aminopeptidase gene pepC. FEMS Microbiol Lett 124, 291-300.
13. Klein, J. R., Ulrich, C., Wegmann, U., Meyer-Barton, E., Plapp, R. & Henrich, B. (1996). Molecular tools for the genetic modification of dairy lactobacilli. System Appl Microbiol 18, 493-503.
14. Kok, J. & De Vos, W. (1994). The proteolytic system of lactic acid bacteria. In Genetics and Biotechnology of Lactic Acid Bacteria, pp. 169-210. Edited by M. J. Gasson & W. M. De Vos. Glasgow : Blackie.
15. Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
16. Magdolen, U., Mueller, G., Magdolen, V. & Bandlow, W. (1993). A yeast gene (BLH1) encodes a polypeptide with high homology to vertebrate bleomycin hydrolase, a family member of thiol proteases. Biochim Biophys Acta 1171, 299-303.
17. Matern, H. T., Klein, J. R., Henrich, B. & Plapp, R. (1994). Determination and comparison of Lactobacillus delbrueckii subsp. lactis DSM7290 promoter sequences. FEMS Microbiol Lett 122, 121-128.
18. Miller, C. & Schwartz, G. (1978). Peptidase deficient mutants of Escherichia coli. J Bacteriol 135, 603-611.
19. O'Callaghan, D. & Charbit, A. (1990). High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation. Mol Gen Genet 223, 156-158.
20. Pearson, W. R. & Lipman, D. J. (1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85, 2444-2488.
21. Poolman, B., Kunji, E. R. S., Hagting, A., Juillard, V. & Konings, W. L. (1995). The proteolytic pathway of Lactococcus lactis. J Appl Bacteriol Symp Suppl 79, 65S-75S.
22. Pritchard, G. G. & Coolbear, T. (1993). The physiology and biochemistry of the proteolytic system in lactic acid bacteria. FEMS Microbiol Rev 12, 179-206.
23. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
24. Sanger, F., Nicklen, S. & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.
25. Sankar, P., Hutton, M. E., VanBogelen, R. A., Clark, R. L. & Neidhardt, F. C. (1993). Expression analysis of cloned chromosomal segments of Escherichia coli. J Bacteriol 175, 5145-5152 (author's correction J Bacteriol 176, 939).
26. Shine, J. & Dalgarno, L. (1974). The 3′-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci USA 71, 1342-1346.
27. Stoker, N. G., Fairweather, N. F. & Spratt, B. G. (1982). Versatile low copy number plasmid vectors for cloning in Escherichia coli. Gene 18, 335-341.
28. Stucky, K., Hagting, A., Klein, J. R., Matern, H., Henrich, B., Konings, W. N. & Plapp, R. (1995a). Cloning and characterization of brnQ, a low affinity branched chain amino acid carrier gene from Lactobacillus delbrückü subsp. lactis DSM7290. Mol Gen Genet 249, 682-690.
29. Stucky, K., Klein, J. R., Schüller, A., Matern, H., Henrich, B. & Plapp, R. (1995b). Cloning and DNA sequence analysis of pepQ, a prolidase gene from Lactobacillus delbrueckii subsp. lactis DSM7290 and partial characterization of its product. Mol Gen Genet 247, 494-500.
30. Vesanto, E., Varmanen, P., Steele, J. L. & Palva, A. (1994). Characterization and expression of the Lactobacillus helveticus pepC gene encoding a general aminopeptidase. Eur J Biochem 224, 991-997.
31. Vesanto, E., Savijoki, K., Rantanen, T., Steele, J. L. & Palva, A. (1995). An X-Prolyl dipeptidyl aminopeptidase (pepX) gene from Lactobacillus helveticus. Microbiology 141, 3067-3075.
32. Wang, R. F. & Kushner, S. R. (1991). Construction of versatile low-copy-number vectors for cloning, sequencing and gene expression in Escherichia coli. Gene 100, 195-199.
33. Weber, K. & Osborn, M. (1969). The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gel electrophoresis. J Biol Chem 244, 4406-4412.