Molecular biological methods to detect 'Microthrix parvicella' and to determine its abundance in activated sludge

Water Science and Technology

Volumn 37, Issue 4-5, 1998, Pages 37-45

  Bradford, Debbie ^a   Christensson, Camilla ^b   Jakab, Nicole ^a   Blackall, Linda L ^a  

^a UNIVERSITY OF QUEENSLAND   (Australia)

^b LUND UNIVERSITY   (Sweden)

Author keywords

'Microthrix parvicella'; 16S rRNA; 23S rRNA; Hybridization; Molecular methods; Nucleic acid; PCR; Quantitative method

Indexed keywords

BULKING; HYBRIDIZATION; MOLECULAR BIOLOGY;

BACTERIA; GENES; NUCLEIC ACID SEQUENCES; RNA;

ACTIVATED SLUDGE PROCESS;

BACTERIAL RNA; NUCLEIC ACID; NYLON; RIBOSOME DNA; RIBOSOME RNA;

ACTIVATED SLUDGE; BACTERIUM; BIOTECHNOLOGY;

ACTIVATED SLUDGE; BACTERIUM DETECTION; CHEMOLUMINESCENCE; CONFERENCE PAPER; DENSITOMETRY; GENE SEQUENCE; GRAM POSITIVE BACTERIUM; HYBRIDIZATION; MEMBRANE; MICROTHRIX; MOLECULAR BIOLOGY; NONHUMAN; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; STRAIN DIFFERENCE;

BACTERIA (MICROORGANISMS); CANDIDATUS MICROTHRIX PARVICELLA; POSIBACTERIA;

EID: 0031831725     PISSN: 02731223     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0273-1223(98)00081-X     Document Type: Conference Paper

References (27)

1. Amann, R. I., Krumholz, L. and Stahl, D. A. (1990). Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol., 172, 762-770.
2. Blackall, L. L. (1994). Molecular identification of activated sludge foaming bacteria. Wat. Sci. Tech., 29(7), 35-42.
3. Blackall, L. L., Burrell, P. C., Gwilliam, H., Bradford, D., Bond, P. L. and Hugenholtz, P. (1997). The use of 16S rDNA clone libraries to describe the microbial diversity of activated sludge communities. Wat. Sci. Tech., 37(4-5), xxx-xxx, (this issue).
4. Blackall, L. L., Seviour, E. M., Cunningham, M. A., Seviour, R. J. and Hugenholtz, P. (1994). "Microthrix parvicella" is a novel, deep branching member of the actinomycetes subphylum. System. Appl. Microbiol., 17, 513-518.
5. Blackall, L. L., Stratton, H., Bradford, D., Sjörup, C., Del Dot, T., Seviour, E. M. and Seviour, R. J. (1996). "Candidatus Microthrix parvicella" - a filamentous bacterium from activated sludge sewage treatment plants. Int. J. Syst. Bacteriol., 4, 344-346.
6. Bond, P. L., Hugenholtz, P., Keller, J. and Blackall, L. L. (1995). Bacterial community structures of phosphate-removing and non-phosphate-removing activated sludges from sequencing batch reactors. Appl. Environ. Microbiol., 61, 1910-1916.
7. Chun, J., Blackall, L. L., Kang, S.-O., Hah, Y. C. and Goodfellow, M. (1997). A proposal to reclassify Nocardia pinensis (Blackall et al.) as Skermania piniformis gen. nov., comb. nov. Int. J. Syst. Bacteriol., 47, 127-131.
8. Eikelboom, D. H. and van Buijsen, H. J. J. (1983). Microscopic Sludge Investigation Manual. TNO, Delft, The Netherlands.
9. Erhart, R., Bradford, D., Seviour, R. J., Amann, R. I. and Blackall, L. L. (in press). Development and use of fluorescent in situ hybridization probes for the detection and identification of "Microthrix parvicella" in activated sludge. System. Appl. Microbiol.
10. Jenkins, D., Richard, M. G. and Daigger, G. T. (1993). Manual on the Causes and Control of Activated Sludge Bulking and Foaming. Lewis Publishers, New York.
11. Lane, D. J. (1991). 16S/23S rRNA sequencing. In: Nucleic Acid Techniques in Bacterial Systematics, E. Stackebrandt and M. Goodfellow (Eds), Academic Press, Chichester, pp. 115-175.
12. Ludwig, W. G. K., Klugbauer, N., Weizenegger, M., Betzl, D., Ehrmann, M., Hertel, C., Jilg, S., Tatzel, R., Zitzelsberger, H., Liebl, S., Hochberer, M., Shah, J., Lane, D., Wallnofer, P. R. and Schleifer, K.-H. (1992). Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+C content. System. Appl. Microbiol., 15, 487-501.
13. Manz, W., Amann, R., Ludwig, W., Wagner, M. and Schleifer, K.-H. (1992). Phylogenetic oligonucleotide probes for the major subclasses of Proteobacteria: problems and solutions. System. Appl. Microbiol., 15, 593-600.
14. Pipes, W. O. (1979). Bulking, deflocculation and pinpoint floe. J. Wat. Poll. Cont. Fed., 51, 62-70.
15. Pitt, P. and Jenkins, D. (1990). Causes and control of Nocardia foaming in activated sludge. Res. J. Wat. Poll. Cont. Fed., 62, 143-150.
16. Raskin, L., Amann, R. I., Poulsen, L. K., Rittmann, B, E. and Stahl, D. A. (1995). Use of ribosomal RNA-based molecular probes for characterization of complex microbial communities in anaerobic biofilms. Wat. Sci. Tech., 31(1), 261-272.
17. Raskin, L., Capman, W. C., Kane, M. D., Rittmann, B. E. and Stahl, D. A. (1996). Critical evaluation of membrane supports for use in quantitative hybridizations. Appl. Environ. Microbiol., 62, 300-303.
18. Reasoner, D. J. and Geldreich, E. E. (1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl. Environ. Microbiol., 49, 1-7.
19. Roller, C., Ludwig, W. and Schleifer, K.-H. (1992). Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes. J. Gen. Microbiol, 138, 1167-1175.
20. Roller, C, Wagner, M., Amann, R., Ludwig, W. and Schleifer, K.-H. (1994). In situ probing of Gram-positive bacteria with high DNA G+C content using 23S rRNA-targeted oligonucleotides. Microbiol., 140, 2849-2858.
21. Rossetti, S., Christensson, C., Blackall, L. L. and Tandoi, V. (1997). Phenotypic and phylogenetic description of an Italian isolate of "Microthrix parvicella". J. Appl. Microbiol., 82, 405-410.
22. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Springs Harbour Laboratory Press, Cold Springs Harbour, NY.
23. Sezgin, M., Jenkins, D. and Parker, D. S. (1978). A unified theory of filamentous sludge bulking. J. Wat. Poll Cont. Fed., 50, 362-381.
24. Stahl, D. A., Flesher, B., Mansfield, H. R. and Montgomery, L. (1988). Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology. Appl. Environ. Microbiol., 54, 1079-1084.
25. van Camp, G., Chapelle, S. and de Wachter, R. (1993). Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences. Curr. Microbiol., 27, 147-151.
26. van Veen, W. L. (1973). Bacteriology of activated sludge, in particular the filamentous bacteria. Antonie van Leeuwenhoek, 39, 189-205.
27. Wagner, M., Erhart, R., Manz, W., Amann, R., Lemmer, H., Wedi, D. and Schleifer, K.-H. (1994). Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge. Appl. Environ. Microbiol., 60, 792-800.