Highly processive motility is not a general feature of the kinesins

European Biophysics Journal

Volumn 27, Issue 4, 1998, Pages 353-360

  Stewart, Russell J ^a   Semerjian, Jessica ^c   Schmidt, Christoph F ^b  

^a Department of Electrical and Computer Engineering   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c NONE

Author keywords

In vitro motility; Kinesins; Microtubules; Ned protein; Processivity

Indexed keywords

CYTOSKELETON PROTEIN; KINESIN; METHYLCELLULOSE; NCD PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; MEMBRANE TRANSPORT; MICROTUBULE; PROTEIN BINDING; PROTEIN PROCESSING;

ADENOSINE TRIPHOSPHATE; ADENYLYL IMIDODIPHOSPHATE; ANIMALS; BIOPHYSICS; CATTLE; DECAPODIFORMES; DROSOPHILA PROTEINS; KINESIN; MICROTUBULES; MOVEMENT; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS;

EID: 0031826857     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490050142     Document Type: Article

References (34)

1. Berliner E, Young EC, Anderson K, Mahtani HK, Gelles J (1995) Failure of a single-headed kinesin to track parallel to microtubule protofilaments. Nature 373: 718-721
2. Block SM, Goldstein LS, Schnapp BJ (1990) Bead movement by single kinesin molecules studied with optical tweezers. Nature 348:348-352
3. Case RB, Pierce DW, HomBooher N, Hart CL, Vale RD (1997) The directional preference of kinesin motors is specified by an element outside of the motor catalytic domain. Cell 90:959-966
4. Chandra R, Salmon ED, Erickson HP, Lockhart A, Endow SA (1993) Structural and fucntional domains of the Drosophila ncd microtubule motor protein. J Biol Chem 268: 9005-9013
5. Choppin CM, Finer JT, Spudich JA, Vale RD (1996) Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy. Proc Natl Acad Sci USA 93: 1913-1917
6. Endow Sa, Kang SJ, Satterwhite LL, Rose MD, Skeen VP, Salmon ED (1994) Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J 13: 2708-2713
7. Finer JT, Simmons RM, Spudich JA (1994) Single myosin molecule mechanics: piconewton forces and nanometre steps. Nature 368: 113-119
8. Gho M, McDonald K, Ganetzky B, Saxton WM (1992) Effects of kinesin mutations on neuronal functions. Science 258: 313-316
9. Gilbert SP, Webb MR, Brune M, Johnson KA (1995) Pathway of processive ATP hydrolysis by kinesin. Nature 373: 671-676
10. Gittes F, Meyhöfer E, Sung B, Howard J (1996) Directional loading of the kinesin motor molecule at it buckles a microtubule. Biophys J 70: 418-429
11. Hackney DD (1994) Evidence for alternating head catalysis by kinesin during microtubule-stimulated ATP hydrolysis. Proc Natl Acad Sci USA 91: 6865-6869
12. Hackney DD (1995) Highly processive microtubule-stimulated ATP hydrolysis by dimeric kinesin head domains. Nature 377: 448-450
13. Harada Y, Sakurada K, Aoki T, Thomas DD, Yanagida T (1990) Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay. J Mol Biol 216: 49-68
14. Hatsumi M, Endow SA (1992) Mutants of the microtubule motor protein, nonclaret disjunctional, affect spindle structure and chromosome movement in meiosis and mitosis. J Cell Sci 101: 547-559
15. Henningsen U, Schliwa M (1997) Reversal in the direction of movement of a molecular motor. Nature 389: 93-96
16. Howard J, Hudspeth AJ, Vale RD (1989) Movement of microtubules by single kinesin molecules. Nature 342: 154-158
17. Kimble M, Church K (1983) Meiosis and early cleavage in Drosophila melanogaster eggs: effects of the claret-non-disjunctional mutation. J Cell Sci 62: 301-318
18. McDonald HB, Goldstein LS (1990) Identification and characterization of a gene encoding a kinesin-like protein in Drosophila. Cell 61: 991-1000
19. McTigue MA, Williams DR, Tainer JA (1995) Crystal structure of a schistosomal drug and vaccine traget: glutathione S-transferase from Schistosoma japonica and its complex with the leading antoschistosomal drug praziquantel. J Mol Biol 246: 21-27
20. Meyhöfer E, Howard J (1995) The force generated by a single kinesin molecule against an elastic load. Proc Natl Acad Sci USA 92: 574-578
21. Molloy JE, Burns JE, Kendrick-Jones J, Tregear RT, White DC (1995) Movement and force produced by a single myosin head. Nature 378: 209-212
22. Moore JD, Endow SA (1996) Kinesin proteins: a phylum of motors for microtubule-based motility. Bioessays 18: 207-219
23. Press WH, Flannery BP, Teukolsky SA, Vetterling WT (1992) Numerical recipes in C: the art of scientific computing, 2nd edn. Cambridge University Press, Cambridge, New York
24. Ray S, Meyhöfer E, Milligan RA, Howard J (1993) Kinesin follows the microtubule's protofilament axis. J Cell Biol 121: 1083-1093
25. Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusion with glutathione S-transferase. Gene 67: 31-40
26. Stewart RJ, Thaler JP, Goldstein LS (1993) Direction of microtubule movement is an intrinsic property of the motor domains of kinesin heavy chain and Drosophila ncd protein. Proc Natl Acad Sci USA 90: 5209-5213
27. Svoboda K, Schmidt CF, Schnapp BJ, Block SM (1993) Direct observation of kinesin stepping by optical trapping interferometry. Nature 365: 721-727
28. Toyoshima YY, Kron SJ, Spudich JA (1990) The myosin step size: measurement of the unit displacement per ATP hydrolyzed in an in vitro assay. Proc Natl Acad Sci USA 87: 7130-7134
29. Uyeda TQ, Kron SJ, Spudich JA (1990) Myosin step size. Estimation from slow sliding movement of actin over low densities of heavy meromyosin. J Mol Biol 214: 699-710
30. Vale RD, Funatsu T, Pierce DW, Romberg L, Harada Y, Yanagida T (1996) Direct observation of single kinesin molecules moving along microtubules. Nature 380: 451-453
31. Vale RD, Reese TS, Sheetz MP (1985) Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility. Cell 42: 39-50
32. Walker RA, Salmon ED, Endow SA (1990) The Drosophila claret segregation protein is a minus-end directed motor molecule [see comments]. Nature 347: 780-782
33. Wang Z, Khan S, Sheetz MP (1995) Single cytoplasmic dynein molecule movements: characterization and comparison with kinesin. Biophys J 69: 2011-2023
34. Williams Jr. RC, Lee JC (1982) Preparation of tubulin from brain. In: Frederiksen DW, Cunningham LW (eds) Structural and Contractile Proteins (Part B: The contractile apparatus and the cytoskeleton). Methods in Enzymology, vol 85. Academic Press, San Diego, pp 376-385