Generation of Escherichia coli intimin derivatives with differing biological activities using site-directed mutagenesis of the intimin C-terminus domain

Molecular Microbiology

Volumn 29, Issue 2, 1998, Pages 559-570

  Frankel, Gad ^a   Philips, Alan D ^b   Novakova, Michaela ^a   Batchelor, Miranda ^a   Hicks, Susan ^b   Dougan, Gordon ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b ROYAL FREE HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

OUTER MEMBRANE PROTEIN;

ARTICLE; BACTERIAL COLONIZATION; CELL ADHESION; CELL INVASION; CITROBACTER; CONTROLLED STUDY; DIARRHEA; ESCHERICHIA COLI; HEP 2 CELL; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINE; MICROVILLUS; MUTANT; NONHUMAN; PATHOGENICITY; PLASMID; PRIORITY JOURNAL;

ACTINS; ADHESINS, BACTERIAL; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BINDING SITES; CARCINOMA, HEPATOCELLULAR; CARRIER PROTEINS; CELL ADHESION; CHILD; CHILD, PRESCHOOL; CITROBACTER; COLON; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FEMALE; HUMANS; INFANT; INTESTINES; MALE; MICE; MUTAGENESIS, SITE-DIRECTED; MUTATION; TUMOR CELLS, CULTURED;

BACTERIA (MICROORGANISMS); CITROBACTER RODENTIUM; ESCHERICHIA COLI; MURINAE; NEGIBACTERIA;

EID: 0031824077     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1998.00950.x     Document Type: Article

References (42)

1. Adu-Bobie, J., Frankel, G., Bain, C., Goncalves, A.G., Trabulsi, L.R., Douce, G., et al. (1998) Intimin α, β, γ, and δ: four intimin derivatives expressed by attaching and effacing microbial pathogens. J Clin Microbiol 36: 662-668.
2. Barthold, S.W., Coleman, G.L., Bhatt, P.N., Osbasdiston, G.W., and Jonas, A.M. (1976) The etiology of transmissible murine colonic hyperplasia. Lab Anim Sci 26: 889-894.
3. Ben-Ami, G., Ozeri, V., Hanski, E., Hofmann, F., Aktories, K., Hahn, K.M., et al. (1998) Agents that inhibit Rho, Rac, and Cdc42 do not block formation of actin pedestals in HeLa cells infected with enteropathogenic Escherichia coli. Infect Immun 66: 1755-1758.
4. Donnenberg, M.S., and Kaper, J.B. (1991) Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59: 4310-4317.
5. Donnenberg, M.S., Yu, J., and Kaper, J.B. (1993a) A second chromosomal gene necessary for intimate attachment of enteropathogenic Escherichia coli to epithelial cells. J Bacteriol 175: 4670-4680.
6. Donnenberg, M.S., Tacket, C.O., James, S.P., Losonsky, G., Nataro, J.P., Wasserman, S.S., et al. (1993b) Role of the eae gene in experimental enteropathogenic Escherichia coli infection. J Clin Invest 92: 1412-1417.
7. Donnenberg, M.S., Kaper, J.B., and Finlay, B.B. (1997) Interaction between enteropathogenic Escherichia coli and host epithelial cells. Trends Microbiol 5: 109-114.
8. Fagundes Neto, U., Freymuller, E., Gatti, M.S., Schmitz, L.G., and Scaletsky, I. (1995) Enteropathogenic Escherichia coli O111ab:H2 penetrates the small bowel epithelium in an infant with acute diarrhoea. Acta Paediatr 84: 453-455.
9. Francis, C.L., Jerse, A.E., Kaper, J.B., and Falkow, S. (1991) Characterization of interactions of enteropathogenic Escherichia coli O127:H6 with mammalian cells in vitro. J Infect Dis 164: 693-703.
10. Frankel, G., Candy, D.C., Everest, P., and Dougan, G. (1994) Characterization of the C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei. Infect Immun 62: 1835-1842.
11. Frankel, G., Candy, D.C.A., Fabiani, E., Adu-Bobie, J., Gil, S., Novakova, M., et al. (1995) Molecular characterization of a carboxy-terminal eukaryotic-cell-binding domain of intimin from enteropathogenic Escherichia coli. Infect Immun 63: 4323-4328.
12. 1 integrins. J Biol Chem 271: 20359-20364.
13. Frankel, G., Phillips, A.D., Adu-Bobie, J., Candy, D.C.A., Douce, G., and Dougan, G. (1996b) Intimin-mediated adherence to epithelial cells. Rev Microbiol Sao Paulo 27 (suppl. 1): 99-103.
14. Frankel, G., Phillips, A.D., Novakova, M., Candy, D.C.A., Schauer, D.B., Douce, G., et al. (1996c) Intimin from enteropathogenic Escherichia coli restores murine virulence to a Citrobacter rodentium eaeA mutant: induction of an immunoglobulin A response to intimin and EspB. Infect Immun 64: 5315-5325.
15. Giron, J.A., Ho, A.S., and Schoolnik, G.K. (1991) An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254: 710-713.
16. Hicks, S., Candy, D.C.A., and Phillips, A.D. (1996) Adhesion of enteroaggregative Escherichia coli to paediatric intestinal mucosa in vitro. Infect Immun 64: 4751-4760.
17. Hicks, S., Frankel, G., Kaper, J.B., Dougan, G., and Phillips, A.D. (1998) Role of intimin and bundle-forming pili in enteropathogenic Escherichia coli adhesion to paediatric intestine in vitro. Infect Immun 66: 1473-1481.
18. Isberg, R.R., and Leong, J.M. (1990) Multiple β1 chain integrins are receptors for invasin, a protein that promotes bacterial penetration into mammalian cells. Cell 60: 861-871.
19. Isberg, R.R., Voorhis, D.L., and Falkow, S. (1987) Identification of invasin: a protein that allows enteric bacteria to penetrate cultured mammalian cells. Cell 50: 769-778.
20. Isberg, R.R., Yang, Y., and Voorhis, D.L. (1993) Residues added to the carboxyl terminus of the Yersinia pseudotuberculosis invasin protein interfere with recognition by integrin receptors. J Biol Chem 268: 15840-15846.
21. Jarvis, K.G., Giron, J.A., Jerse, A.E., McDaniel, T.K., Donnenberg, M.S., and Kaper, J.B. (1995) Enteropathogenic Escherichia coli contains a putative type III secretion system necessary for the export of proteins involved in attaching and effacing lesion formation. Proc Natl Acad Sci USA 92: 7996-8000.
22. Jerse, A.E., Yu, J., Tall, B.D., and Kaper, J.B. (1990) A genetic locus of enteropathogenic Escherichia coli necessary for the production of attaching and effacing lesions on tissue culture cells. Proc Natl Acad Sci USA 87: 7839-7843.
23. Kelly, G., Prasannan, S., Daniel, S., Frankel, G., Dougan, G., Connerton, I., et al. (1998) Sequential assignment of the triple labelled 30.1 kDa cell adhesion domain of intimin from enteropathogenic E. coli. J Biomol NMR (in press).
24. Kenny, B., Lai, L.C., Finlay, B.B., and Donnenberg, M.S. (1996) EspA, a protein secreted by enteropathogenic Escherichia coli, is required to induce signals in epithelial cells. Mol Microbiol 20: 313-323.
25. Kenny, B., DeVinney, R., Stein, M., Reinscheild, D.J., Frey, E.A., and Finlay, B.B. (1997) Enteropathogenic E. coli (EPEC) transfers its receptor for intimate adherence into mammalian cells. Cell 91: 511-520.
26. Knutton, S., Lloyd, D.R., and McNeish, A.S. (1987) Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa. Infect Immun 55: 69-77.
27. Knutton, S., Baldwin, T., Williams, P.H., and McNeish, A.S. (1989) Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli. Infect Immun 57: 1290-1298.
28. Knutton, S., Adu-Bobie, J., Bain, C., Phillips, A.D., Dougan, G., and Frankel, G. (1997) Down regulation of intimin expression during attaching and effacing enteropathogenic Escherichia coli adhesion. Infect Immun 65: 1644-1652.
29. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
30. Lai, L.C., Wainwright, L.A., Stone, K.D., and Donnenberg, M.S. (1997) A third secreted protein that is encoded by the enteropathogenic Escherichia coli pathogenicity island is required for transduction of signals and for attaching and effacing activities in host cells. Infect Immun 65: 2211-2217.
31. Leong, J.M., Fournier, R.S., and Isberg, R.R. (1990) Identification of the integrin binding domain of the Yersinia pseudotuberculosis invasin protein. EMBO J 9: 1979-89.
32. Leong, J.M., Morrissey, P.E., and Isberg, R.R. (1993) A 76-amino acid disulfide loop in the Yersinia pseudotuberculosis invasin protein is required for integrin receptor recognition. J Biol Chem 268: 20524-20532.
33. Levine, M.M. (1987) Escherichia coli that cause diarrhea: enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic, and enteroadherent. J Infect Dis 155: 377-389.
34. McDaniel, T.K., and Kaper, J.B. (1997) A cloned pathogenicity island from enteropathogenic Escherichia coli confers the attaching and effacing phenotype on E. coli K-12. Mol Microbiol 23: 399-407.
35. McDaniel, T.K., Jarvis, K.G., Donnenberg, M.S., and Kaper, J.B. (1995) A genetic locus of enterocyte effacement conserved among diverse enterobacterial pathogens. Proc Natl Acad Sci USA 92: 1664-1668.
36. Moon, H.W., Whipp, S.C., Argenzio, R.A., Levine, M.M., and Giannella, R.A. (1983) Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines. Infect Immun 41: 1340-1351.
37. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
38. Schauer, D.B., and Falkow, S. (1993a) Attaching and effacing locus of a Citrobacter freundii biotype 4280 that causes transmissible murine colonic hyperplasia. Infect Immun 61: 2486-2492.
39. Schauer, D.B., and Falkow, S. (1993b) The eae gene of Citrobacter freundii biotype 4280 is necessary for colonization in transmissible murine colonic hyperplasia. Infect Immun 61: 4654-4661.
40. Tran Van Nhieu, G., and Isberg, R.R. (1993) Bacterial internalization mediated by beta 1 chain integrins is determined by ligand affinity and receptor density. EMBO J 12: 1887-1895.
41. Ulshen, M.H., and Rollo, J.L. (1980) Pathogenesis of Escherichia coli gastroenteritis in man - another mechanism. N Engl J Med 302: 99-101.
42. Wolff, C., Nisan, I., Hanski, E., Frankel, G., and Rosenshine, I. (1998) Protein translocation into epithelial cells by infecting enteropathogenic Escherichia coli. Mol Microbiol 28: 143-155.