Interactions between multiple phosphorylation sites in the inactivation particle of a K+ channel: Insights into the molecular mechanism of protein kinase C action

Journal of General Physiology

Volumn 112, Issue 1, 1998, Pages 71-84

  Beck, Edward J ^a   Sorensen, Roger G ^a   Slater, Simon J ^a   Covarrubias, Manuel ^a  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

Author keywords

Inactivation gating; Potassium channels; Protein phosphorylation; Site directed mutagenesis; Structure function relation

Indexed keywords

POTASSIUM CHANNEL; PROTEIN KINASE C;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STRUCTURE; NONHUMAN; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; ASPARTIC ACID; ION CHANNEL GATING; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OOCYTES; PATCH-CLAMP TECHNIQUES; PHOSPHORYLATION; POTASSIUM CHANNELS; PROTEIN KINASE C; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP; XENOPUS;

EID: 0031823948     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.112.1.71     Document Type: Article

References (44)

1. Andersen, O.S., and R.E. Koeppe II. 1992. Molecular determinants of channel function. Physiol. Rev. 72: S89-S158.
2. Antz, C., M. Geyer, B. Fakler, M.K. Schott, R. Guy, R. Frank, J.P. Ruppersberg, and H.R. Kalbitzer. 1997. NMR structure of inactivation gates from mammalian voltage-dependent potassium channels. Nature. 385:272-274.
3. + channels mediated by protein phosphorylation. Biophys. J. 74: A229. (Abstr.)
4. +]: a tale of two inactivation mechanisms. Neuron. 15:951-960.
5. Carter, P.J., G. Winter, A.J. Wilkinson, and A.R. Fersht. 1984. The use of double mutants to detect structural changes in the active site of the tyrosyl-tRNA synthetase (Bacillus stearothermophilus). Cell. 38:835-840.
6. Cline, J., J.C. Braman, and H.H. Hogrefe. 1996. PCR fidelity of Pfu polymerase and other thermostable DNA polymerases. Nucleic Acids Res. 24:3546-3551.
7. Covarrubias, M., A. Wei, L. Salkoff, and T.B. Vyas. 1994. Elimination of rapid potassium channel inactivation by phosphorylation of the inactivation gate. Neuron. 13:1403-1412.
8. Covarrubias, M., E.J. Beck, A. Matlapudi, and R.G. Sorensen. 1997. Phosphorylation at multiple N-terminal sites directly modulates inactivation of Kv3.4. Biophys. J. 72:341. (Abstr.)
9. + channel behaves like an open-channel blocker. Neuron. 7:743-753.
10. + channel inactivation by cAMP-dependent protein kinase. Neuron. 12:1097-1109.
11. Flaman, J.-M., T. Frebourg, V. Moreau, F. Charbonnier, C. Martin, C. Ishioka, S.H. Friend, and R. Iggo. 1994. A rapid PCR fidelity assay. Nucleic Acids Res. 22:3259-3260.
12. Fothergill, M.D., and A.R. Fersht. 1991. Correlation between kinetic and x-ray analyses of engineered enzymes: crystal structure of mutants Cys-Gly-35 and Tyr-Phe-34 of tyrosyl-tRNA synthetase. Biochemistry. 30:5157-5164.
13. + channels: a test for the number of inactivating particles on each channel. Biophys. J. 68:89-95.
14. Gutfreund, H. 1995. Kinetics for the Life Sciences. 1st ed. Cambridge University Press, Melbourne, Australia. 231-280.
15. Hille, B. 1992. Ionic Channels of Excitable Membranes. 2nd ed. Sinauer Associates Inc., Sunderland, MA. 116-121.
16. + channel regulation of signal propagation in dendrites of hippocampal pyramidal neurons. Science. 387:869-875.
17. Hoshi, T., W.N. Zagotta, and R.W. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-568.
18. + channels: effects of alterations in the carboxy-terminal region. Neuron. 7:547-556.
19. + channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains. Biophys. J. 72:163-174.
20. Johnson, L.N., and D. Barford. 1993. The effects of phosphorylation on the structure and function of proteins. Annu. Rev. Biophys. Biomol. Struct. 22:199-232.
21. Johnson, L.N., and M. O'Reilly. 1996. Control by phosphorylation. Curr. Opin. Struct. Biol. 6:762-769.
22. Jonas, E.A., and L.K. Kaczmarek. 1996. Regulation of potassium channels by protein kinases. Curr. Opin. Neurobiol. 6:318-323.
23. Kandel, E.R., J.H. Schwartz, and T.M. Jessel. 1995. Essentials of Neural Science and Behavior. Appleton & Lange, Norwalk, CT. 243-692.
24. + channels determined by an interacting pair of nonpolar amino acids. Neuron. 8:499-505.
25. Kunkel, T.A. 1988. Exonucleolytic proofreading. Cell. 53:837-840.
26. Kupper, J., M.R. Bowlby, S. Marom, and I.B. Levitan. 1995. Intracellular and extracellular aminoacids that influence C-type inactivation and its modulation in a voltage-dependent potassium channel. Pflügers Arch. 430:1-11.
27. Levitan, I.B. 1994. Modulation of ion channels by protein phosphorylation and dephosphorylation. Annu. Rev. Physiol. 56:193-212.
28. Lopez-Barneo, J., T. Hoshi, S.H. Heinemann, and R.W. Aldrich. 1993. Effects of external cations and mutations in the pore region on C-type inactivation of Shaker potassium channels. Receptors Channels. 1:61-71.
29. Lundberg, K.S., D.D. Shoemaker, M.W.W. Adams, J.M. Short, J.A. Sorge, and E.J. Mathur. 1991. High fidelity amplification using thermostable DNA polymerase isolated from Pyrococcus furiosus. Gene (Amst.). 108:1-6.
30. + channels with a pore blocking site studied with synthetic peptides. J. Gen. Physiol. 102: 949-975.
31. + channels blocked by inactivation peptides. J. Gen. Physiol. 102:977-1003.
32. Nicoll, R.A. 1988. The coupling of neurotransmitter receptors to ion channels in the brain. Science. 241:545-551.
33. Nishizuka, V. 1988. The molecular heterogeneity of protein kinase C and its implications in cellular regulation. Nature. 334:661-665.
34. + channels. Biophys. J. 69:2449-2457.
35. + channel occurs by a cooperative mechanism. Biophys. J. 69:896-903.
36. + channels in the squid giant axon. A mechanistic analysis. J. Bioenerg. Biomembr. 23:599-610.
37. Rettig, J., F. Wunder, M. Stocker, R. Lichtinghagen, F. Mastiaux, S. Beckh, W. Kues, P. Pedarzani, K.H. Schroeter, J.P. Ruppersberg, R. Veh, and O. Pongs. 1992. Characterization of a Shaw-related potassium channel family in rat brain. EMBO (Eur. Mol. Biol. Organ.) J. 11:2473-2488.
38. Roeper, J., and O. Pongs. 1996. Presynaptic potassium channels. Curr. Opin. Neurobiol. 6:338-341.
39. + channel Kv1.4 regulated by Ca/calmodulin-dependent protein kinase. J. Neurosci. 17:3379-3391.
40. Ruppersberg, J.P., R. Frank, O. Pongs, and M. Stocker. 1991. Cloned neuronal Ik(A) channels reopen during recovery from inactivation. Nature. 353:657-660.
41. Slater, S.J., K.J. Cox, J.V. Lombardi, C. Ho. M.B. Kelly, E. Rubin, and C.D. Stubbs. 1993. Inhibition of protein kinase C by alcohols and anesthetics. Nature. 364:82-84.
42. Starkus, J.G., L. Kuschel, M.D. Reyner, and S.H. Heinemann. 1997. Ion conduction through C-type inactivated Shaker channels. J. Gen. Physiol. 110:539-550.
43. +-channel N-type inactivation. Neurobiology (Bp). In press.
44. Tukey, J.W. 1977. Exploratory Data Analysis. Addison-Wesley Publishing Company, Reading, MA. 27-55.