Site-directed mutagenesis of the 19-kilodalton lipoprotein antigen reveals no essential role for the protein in the growth and virulence of Mycobacterium intracellulare

Infection and Immunity

Volumn 66, Issue 8, 1998, Pages 3626-3634

  Mahenthiralingam, Eshwar ^a,b   Marklund, Britt Inger ^c   Brooks, Lucy A ^a,b   Smith, Debbie A ^d   Bancroft, Gregory J ^d   Stokes, Richard W ^a,b  

^a BC CHILDREN S HOSPITAL   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c UMEÅ UNIVERSITY   (Sweden)

^d LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; LIPOPROTEIN; MYCOBACTERIUM ANTIGEN;

ANTIGEN EXPRESSION; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL VIRULENCE; GENE ISOLATION; MYCOBACTERIUM INTRACELLULARE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

MYCOBACTERIUM INTRACELLULARE;

EID: 0031820470     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.66.8.3626-3634.1998     Document Type: Article

References (47)

1. Aldovini, A., R. N. Husson, and R. A. Young. 1993. The uraA locus and homologous recombination in Mycobacterium bovis BCG. J. Bacteriol. 175: 7282-7289.
2. Andersen, A. B., and P. Brennan. 1994. Proteins and antigens of Mycobacterium tuberculosis, p. 307-332. B. R. Bloom (ed.), In Tuberculosis: pathogenesis, protection, and control. American Society for Microbiology, Washington, D.C.
3. Anonymous. 1972. Trudeau Mycobacterial Culture Collection catalog. National Institute for Allergy and Infectious Diseases, Bethesda, Md.
4. Ashbridge, K. R., R. J. Booth, J. D. Watson, and R. B. Lathigra. 1989. Nucleotide sequence of the 19kDa antigen from Mycobacterium tuberculosis. Nucleic Acids Res. 17:1249.
5. Azad, A. K., T. D. Sirakova, L. M. Rogers, and P. E. Kolattukudy. 1996. Targeted replacement of the mycocerosic acid synthase gene in Mycobacterium bovis BCG produces a mutant that lacks mycosides. Proc. Natl. Acad. Sci. USA 93:4787-4792.
6. Balasubramanian, V., M. S. Pavelka, Jr., S. S. Bardarov, J. Martin, T. R. Weisbrod, R. A. McAdam, B. R. Bloom, and W. R. Jacobs, Jr. 1996. Allelic exchange in Mycobacterium tuberculosis with long linear recombination substrates. J. Bacteriol. 178:273-279.
7. Baulard, A., L. Kremer, and C. Locht. 1996. Efficient homologous recombination in fast-growing and slow-growing mycobacteria. J. Bacteriol. 178: 3091-3098.
8. Booth, R. J., D. L. Williams, K. D. Moudgil, L. C. Noonan, P. M. Grandison, J. J. McKee, R. L. Prestidge, and J. D. Watson. 1993. Homologs of Mycobacterium leprae 18-kilodalton and Mycobacterium tuberculosis 19-kilodalton antigens in other mycobacteria. Infect. Immun. 61:1509-1515.
9. Brooks, L. A. Chemical mutagenesis of mycobacteria. In T. Parish and N. Stoker (ed.), Methods in molecular biology: mycobacteria protocols, in press. The Humana Press Inc., Totowa, N.J.
10. Collins, F. M., N. E. Morrison, and V. Montalbine. 1978. Immune response to persistent mycobacterial infection in mice. Infect. Immun. 20:430-438.
11. Collins, F. M., and R. W. Stokes. 1987. Mycobacterium avium-complex infections in normal and immunodeficient mice. Tubercle 68:127-136.
12. Collins, F. M., and S. R. Watson. 1981. Immune responses to atypical mycobacterial lung infections. Rev. Infect. Dis. 3:981-989.
13. Collins, M., A. Paiki, S. Wahl, A. Nolan, J. Goodger, M. Woodward, and J. W. Dale. 1990. Cloning and characterization of the gene for the 19kDa antigen of Mycobacterium bovis. J. Gen. Microbiol. 136:1429-1436.
14. Dunbar, F. P., I. Pejovic, R. Cacciatore, L. Peric-Golia, and E. H. Runyou. 1968. Mycobacterium intracellulare maintenance of pathogenicity in relationship to lyophilization and colony form. Scand. J. Respir. Dis. 49:153-162.
15. Falkinham, J. O. 1996. Epidemiology of infection by nontuberculous mycobacteria. Clin. Microbiol. Rev. 9:177-215.
16. Garbe, T., D. Harris, M. Vordermeier, R. Lathigra, J. Ivanyi, and D. Young. 1993. Expression of the Mycobacterium tuberculosis 19-kilodalton antigen in Mycobacterium smegmatis: immunological analysis and evidence of glycosylation. Infect. Immun. 61:260-267.
17. Hänsch, H. C. R., D. A. Smith, M. E. A. Mielke, H. Hahn, G. J. Bancroft, and S. Ehlers. 1996. Mechanisms of granuloma formation in murine Mycobacterium avium infection: the contribution of CD4+ T cells. Int. Immunol. 8: 1299-1310.
18. Harlow, E., and D. Lane (ed.). 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
19. Harris, D. P., H.-M. Vordermeier, S. J. Brett, G. Pasvol, C. Moreno, and J. Ivanyi. 1994. Epitope specificity and isoforms of the mycobacterial 19-kilodalton antigen. Infect. Immun. 62:2963-2972.
20. Herrmann, J. L., P. O'Gaora, A. Gallagher, J. E. Thole, and D. B. Young. 1996. Bacterial glycoproteins: a link between glycosylation and proteolytic cleavage of a 19 kDa antigen from Mycobacterium tuberculosis. EMBO J. 15: 3547-3554.
21. Husson, R. N., B. E. James, and R. A. Young. 1990. Gene replacement and expression of foreign DNA in mycobacteria. J. Bacteriol. 172:519-524.
22. Inderleid, C. B., C. A. Kemper, and L. E. M. Bermudez. 1993. The Mycobacterium avium complex. Clin. Microbiol. Rev. 6:266-310.
23. Ivanyi, J., and J. E. Thole. 1994. Specificity and function of T and B cell recognition in tuberculosis, p. 437-458. In B. R. Bloom (ed.), Tuberculosis: pathogenesis, protection, and control. American Society for Microbiology, Washington, D.C.
24. Kalpana, G. V., B. R. Bloom, and W. R. Jacobs, Jr. 1991. Insertional mutagenesis and illegitimate recombination in mycobacteria. Proc. Natl. Acad. Sci. USA 88:5433-5437.
25. Lathigra, R., Y. Zhang, M. Hill, M. J. Garcia, P. S. Jackett and J. Ivanyi. 1996. Lack of production of the 19-kDa glycolipoprotein in certain strains of Mycobacterium tuberculosis. Res. Microbiol. 147:237-249.
26. Lee, M. H., L. Pascopella, W. R. Jacobs, Jr., and G. F. Harfull. 1991. Site-specific integration of mycobacteriophage L5: integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guerin. Proc. Natl. Acad. Sci. USA 88:3111-3115.
27. Marklund, B.-I., D. P. Speert, and R. W. Stokes. 1995. Gene replacement through homologous recombination in Mycobacterium intracellulare. J. Bacteriol. 177:6100-6105.
28. Marklund, B.-I., and R. W. Stokes. Unpublished data.
29. McFadden, J. 1996. Recombination in mycobacteria. Mol. Microbiol. 21: 205-211.
30. Nair, J., D. A. Rouse, and S. L. Morris. 1992. Nucleotide sequence analysis and serologic characterization of the Mycobacterium intracellulare homologue of the Mycobacterium tuberculosis 19kDa antigen. Mol. Microbiol. 6: 1431-1439.
31. Norman, E., O. A. Dellagostin, J. McFadden, and J. W. Dale. 1995. Gene replacement by homologous recombination in Mycobacterium bovis BCG. Mol. Microbiol. 16:755-760.
32. Pavelka, M. S., Jr., and W. R. Jacobs, Jr. 1996. Biosynthesis of diaminopimelate, the precursor of lysine and a component of peptidoglycan, is an essential function of Mycobacterium smegmatis. J. Bacteriol. 178:6496-6507.
33. Pelicic, V., J. M. Reyrat, and B. Gicquel. 1996. Positive selection of allelic exchange mutants in Mycobacterium bovis BCG. FEMS Microbiol. Lett. 144: 161-166.
34. Pena, C. E., J. E. Stoner, and G. F. Hatfull. 1996. Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site. J. Bacteriol. 178:5533-5536.
35. Prestidge, R. L., P. M. Grandison, D. W. Chuk, R. J. Booth, and J. D. Watson. 1995. Production of the 19-kDa antigen of Mycobacterium tuberculosis in Escherichia coli and its purification. Gene 164:129-132.
36. Reyrat, J. M., F. X. Berthet, and B. Gicquel. 1995. The urease locus of Mycobacterium tuberculosis and its utilization for the demonstration of allelic exchange in Mycobacterium bovis bacillus Calmette-Guerin. Proc. Natl. Acad. Sci. USA 92:8768-8772.
37. Reyrat, J. M., G. Lopez-Ramirez, C. Ofredo, B. Gicquel, and N. Winter. 1996. Urease activity does not contribute dramatically to persistence of Mycobacterium bovis bacillus Calmette-Guerin. Infect. Immun. 64:3934-3936.
38. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
39. Sander, P., A. Meier, and E. C. Bottger. 1995. rpsL+: a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:991-1000.
40. Schaefer, W. B., C. L. Davis, and M. L. Cohn. 1970. Pathogenicity of transparent, opaque, and rough variants of Mycobacterium avium in chickens and mice. Am. Rev. Respir. Dis. 102:499-506.
41. Schweizer, H. D. 1993. Small broad-host-range gentamycin resistance gene cassette for site specific insertion and deletion mutagenesis. BioTechniques 15:831-834.
42. Snapper, S. B., R. E. Melton, S. Mustafa, T. Kieser, and W. R. Jacobs, Jr. 1990. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol. Microbiol. 4:1911-1919.
43. Thangaraj, H. S., T. J. Bull, K. A. L. De Smet, M. K. Hill, D. A. Rouse, C. Moreno, and J. Ivanyi. 1996. Duplication of the genes encoding the immunodominant 38 kDa antigen in Mycobacterium intracellulare. FEMS Microbiol. Lett. 144:235-240.
44. Young, D. B., and T. R. Garbe. 1991. Lipoprotein antigens of Mycobacterium tuberculosis. Res. Microbiol. 142:55-65.
45. Young, D. B., S. H. Kaufmann, P. W. Hermans, and J. E. Thole. 1992. Mycobacterial protein antigens: a compilation. Mol. Microbiol. 6:133-145.
46. Young, D. B., and K. Duncan. 1995. Prospects for new interventions in the treatment and prevention of mycobacterial disease. Annu. Rev. Microbiol. 49:642-673.
47. Zalacain, M., A. Gonzalez, M. C. Guerrero, R. J. Mattaliano, F. Malpartida, and A. Jimenez. 1986. Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopius. Nucleic Acids Res. 14: 1565-1581.