Embryos cultured in vitro with multinucleated blastomeres have poor implantation potential in human in-vitro fertilization and intracytoplasmic sperm injection

Human Reproduction

Volumn 13, Issue 4, 1998, Pages 960-963

  Pelinck, M J ^a   De Vos, M ^a   Dekens, M ^a   Van der Elst, J ^a   De Sutter, P ^a   Dhont, M ^a  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Human embryo; ICSI; IVF; Multinucleation; Pregnancy

Indexed keywords

ADULT; ARTICLE; BLASTOMERE; CASE CONTROL STUDY; EMBRYO; EMBRYO CULTURE; EMBRYO TRANSFER; FEMALE; FERTILIZATION IN VITRO; HUMAN; INTRACYTOPLASMIC SPERM INJECTION; MAJOR CLINICAL STUDY; MULTINUCLEAR CELL; NIDATION; PREGNANCY RATE; RETROSPECTIVE STUDY;

EID: 0031811732     PISSN: 02681161     EISSN: None     Source Type: Journal    
DOI: 10.1093/humrep/13.4.960     Document Type: Article

References (21)

1. Angell, R.R., Sumner, A.T., West, J.D. et al. (1987) Post fertilization polyploidy in human preimplantation embryos fertilized in-vitro. Hum. Reprod., 2, 721-727.
2. Balakier, H. and Cade sky. K.(l997) The frequency and dewtopmental capability of human embryos containing multinucleated bias to meres. Hum. Reprod., 12, 800-804.
3. Coetsier, T., Verhoeff, A., De Sutler, P. et al. (1997) Transport IVF/ICSI: a prospective randomized study. Hum. Reprod., 12, 1654-1656.
4. Dozortsev, D., De Sutler, P., Rybouchkin, A. et al. (1996) Methodology of intracytoplasmatic sperm injection in the human. Assist. Rep rod. Rev., 6, 38-44.
5. Hardy, K., Martin, K.L., Leese, HJ. et al. (1990) Human preimplantation development in-vitro is nol adversely affecied by biopsy at the 8-cell stage. Hum. Reprod., 5, 708-714.
6. Hardy, K., Winston, R.M.L. and Handyside, A.H. (1993) Binucleate blastomeres in preimplantation human embryos in vitro: failure of cytokinesis during early cleavage. J. Reprod. Fertil., 98, 549-558.
7. Kligman, I., Benadiva, C., Alikani, M. et al. (1996) The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities. Hum, Reprod., 11, 1492-1498.
8. Laverge, H., De Sutler, P., Dhonl, M. et al. (1997) Triple colour fluorescent in-situ hybridization for chromosomes X, Y and 1 on spare human embryos. Hum. Reprod., 12, 809-814.
9. Lopata, A., Kohlman, D. and Johnson, I. (1983) The fine structure of normal and abnormal embryos developed in culture. In Beier, H.M. and Lindner, H.R. (eds), Fertilization of the Human Egg In-Vitro, Springer Verlag, Heidelberg, pp. 189-209.
10. Mohr, L.R., Trounson, A.O., Leeton, J.F. et al. (1983) Evaluation of normal and abnormal human embryo development during procedures in vitro. In Beier, H.M. and Lindner, H.R. (eds), Fertilization of the Human Egg InVitro. Springer Verlag, Heidelberg, pp. 211-222.
11. Munné, S. and Cohen, J. (1993) Unsuitability of multinucleated human blastomeres for preimplantation genetic diagnosis. Hum. Reprod., 8, 1120-1125.
12. Munné, S., Lee, A., Rosewaks, Z. et al. (1993) Diagnosis of major chromosome aneuploidies in human preimplantation embryos. Hum. Reprod., 8, 2185- 2191.
13. Munné, S., Grifo, J., Cohen, J. et al. (1994) Chromosome abnormalities in human arrested preimplantation embryos: a multiple-probe FISH study. Am. J. Hum. Genet., 55, 150-159.
14. Munné, S., Alikani, M., Tomkin, G. et al. (1995a) Embryo morphology, developmental rates and maternal age are correlated with chromosomal abnormalities. Fertil. Steril., 64, 382-391.
15. Munné, S., Sultan, K.M., Weier, H.U.G. et al. (1995b) Assessment of numeric abnormalities of X, Y, 18 and 16 chromosomes in preimplantation human embryos before transfer. Am. J. Obstet. Gynecol, 172, 1191-1201.
16. Pickering, S.J., Braude, P.R., Johnson M.H. et al. (1990) Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Fertil. Steril., 54, 102-108.
17. Pickering, S.J., Taylor, A., Johnson, M.H. et al. (1995) An analysis of multinucleated blastomere formation in human embryos. Moi. Hum. Reprod., 1, Hum. Reprod., 10, 1912-1922.
18. Tesarik, J., Kopecny, V, Plachot, M. et al. (1987) Ultrastructural and autoradiographic observations on multinucleated blastomeres of human cleaving embryos obtained by in-vitro fertilization. Hum. Reprod., 2, 127-136.
19. Tesarik, J. (1988) Developmental control of human preimplantation embryos: a comparative approach. J. In Vitro Fertil. Embryo Transfer, 5, 347-362.
20. Winston, N.J., Braude, PR., Pickering, S.J. et al. (1991) The incidence of abnormal morphology and nucleocytoplasmatic ratios in 2-, 3- and 5-day human pre-embryos. Hum. Reprod., 6, 17-24.
21. Winston, N.J., Johnson, M.H. and Braude, PR. (1993) Assessment of the cellular DNA content of whole mounted mouse and human oocyte s and of blastomeres containing single or multiple nuclei. Zygote, 1, 17-26.