Cell polarity, phagocytosis and viral gene transfer in cultured human retinal pigment epithelial cells

Current Eye Research

Volumn 17, Issue 6, 1998, Pages 668-672

  Da Cruz, Lyndon ^a   Robertson, Terry ^b   Hall, Michael O ^c   Constable, Ian J ^a   Rakoczy, Piroska E ^a  

^a LIONS EYE INSTITUTE   (Australia)

^b UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

^c JULES STEIN EYE INSTITUTE   (United States)

Author keywords

Adenovirus; Gene transfer; Human; Phagocytosis; Retinal pigment epithelium

Indexed keywords

ADENOVIRUS; ANIMAL CELL; ARTICLE; CATTLE; CELL POLARITY; CONTROLLED STUDY; EXPRESSION VECTOR; GENE EXPRESSION; GENE TRANSFER; HUMAN; HUMAN CELL; MONOLAYER CULTURE; NONHUMAN; PHAGOCYTOSIS; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RETINA ROD OUTER SEGMENT; TRANSGENE; VIRUS GENE;

ADENOVIRIDAE; ADOLESCENT; ANIMALS; BETA-GALACTOSIDASE; CELL LINE, TRANSFORMED; CELL POLARITY; CELLS, CULTURED; DEFECTIVE VIRUSES; FLOW CYTOMETRY; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENES, VIRAL; GENETIC VECTORS; HUMANS; PHAGOCYTOSIS; PIGMENT EPITHELIUM OF EYE; RATS; RATS, MUTANT STRAINS; RETINAL DEGENERATION; ROD OUTER SEGMENTS;

EID: 0031809030     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.17.6.668.5175     Document Type: Article

References (21)

1. Zinn KM, Marmor M, eds. Retinal pigment epithelium in human retina. In: The Retinal Pigment Epithelium. Cambridge, MA, USA: Harvard University Press; 1979:32-44.
2. Feeney L, Mixon RN. An in vitro model of phagocytosis in bovine and human retinal pigment epithelium. Exp Eye Res. 1976;22:533-548.
3. Da Cruz L, Rakoczy PE, Perricaudet M, Constable IJ. Dynamics of gene transfer to retinal pigment epithelium. Invest Ophthalmol Vis Sci. 1996;37:2447-2454.
4. Mashhour B, Couton D, Perricaudet M, Briand P. In vivo adenovirus-mediated gene transfer into ocular tissues. Gene Therapy. 1994;1:122-126.
5. Bennett J, Wilson J, Sun D, Forbes B, Maguire A. Adenovirus vector-mediated in vivo gene transfer into adult murine retina. Invest Ophthalmol Vis Sci. 1994;35:2535-2542.
6. Li T, Adamian M, Roof DJ, et al. In vivo transfer of a reporter gene to the retina mediated by an adenoviral vector. Invest Ophthalmol Vis Sci. 1994;35:2543-2549.
7. Budenz DL, Bennett J, Alonso L, Maguire A. In vivo gene transfer into murine corneal endothelial and trabecular meshwork cells. Invest Ophthalmol Vis Sci. 1995;36:2211-2215.
8. Rakoczy P, Lai MC, Watson M, Seydel U, Constable IJ. Targeted delivery of an antisense oligonucleotide in the retina: uptake, distribution, stability and effect. Antisense Nuc Ac Dev. 1996;6:207-213.
9. Bok D, Hall MO. The role of the pigment epithelium in the etiology of inherited retinal dystrophy in the rat. J Cell Biol. 1971;49:664-682.
10. Mullen RJ, LaVail MM. Inherited retinal dystrophy: primary defect in pigment epithelium determined with experimental rat chimeras. Science. 1976;192:799-801.
11. Goldman AI, O'Brien PJ. Phagocytosis in the retinal pigment epithelium of the RCS rat. Science. 1978;201:1023-1025.
12. Edwards RB, Szamier RB. Defective phagocytosis of isolated rod outer segments by RCS rat retinal pigment epithelium in culture. Science. 1977;197:1001-1003.
13. Rakoczy PE, Kennedy C, Thompson-Wallis D, Mann K, Constable IJ. Changes in retinal pigment epithelial cell autofluorescence and protein expression associated with phagocytosis of rod outer segments in vitro. Biol Cell. 1992;76:49-54.
14. Davis AA, Bernstein PS, Bok D, Turner J, Nachtigal M, Hunt RC. A human retinal pigment epithelial cell line that retains epithelial characteristics after prolonged culture. Invest Ophthalmol Vis Sci. 1995;36:955-964.
15. Graham FL, Prevec L. Manipulation of aenovirus actors. In: Murray EJ, ed. Methods in Molecular Biology, 7: Gene Transfer and Expression Protocols. Clifton, NJ: The Humana Press; 1991:109-128.
16. Stratford-Perricaudet LD, Makeh I, Perricaudet M, Briand P. Widespread long-term gene transfer to mouse skeletal muscles and heart. J Clin Invest. 1992;90:626-630.
17. Kennedy CJ, Rakoczy PE, Robertson TA, Papadimitriou JM, Constable IJ. Kinetic studies on phagocytosis and lysosomal digestion of rod outer segments by human retinal pigment epithelial cells in vitro. Exp Cell Res. 1994;210:209-214.
18. Mayerson P, Hall MO, Clark VM, Abrams TA. An improved method for isolation and culture of rat retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1985;26:1599-1609.
19. Kennedy CJ, Rakoczy P, Constable IJ. A simple flow cytometric technique to quantify rod outer segment phagocytosis in cultured retinal pigment epithelial cells. Curr Eye Res. 1996;15:998-1003.
20. Uchida K, Kawaja MD, Toya S, Roach AH. Transgenic neural plate contributes neuronal cells that survive greater than one year when transplanted into the adult mouse central nervous system. Exp Neurol. 1995;132:194-208.
21. Borras T, Tamm ER, Zigler JS. Ocular adenovirus gene transfer varies efficiency and inflammatory response. Invest Ophthalmol Vis Sci. 1996;37:1282-1293.