Identification and verification of differential display cDNAs using gene-specific primers and hybridization arrays

BioTechniques

Volumn 24, Issue 6, 1998, Pages 1018-1026

  Martin, Katherine J ^a,c   Kwan, C P ^a   O'Hare, M J ^b   Pardee, A B ^a   Sager, R ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

^b ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ARTICLE; CONTROLLED STUDY; DNA HYBRIDIZATION; DNA SEQUENCE; GENE EXPRESSION; GENE ISOLATION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION;

EID: 0031801695     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98246cr01     Document Type: Article

References (26)

1. Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts and J.D. Watson. 1989. Molecular Biology of the Cell. Garland, New York.
2. Altschul, S.F., W. Gish, W. Miller, E.W. Myers and D.J. Lipman. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-410.
3. Averboukh, L., S.A. Douglas, S. Zhao, K. Lowe, J. Maher and A.B. Pardee. 1996 Better gel resolution and longer cDNAs increase the precision of differential display. BioTechniques 20:918-921.
4. Band, V. and R. Sager. 1989. Distinctive traits of normal and tumor-derived human mammary epithelial cells expressed in a medium that supports long-term growth of both cell types. Proc. Natl. Acad. Sci. USA 86:1249-1253.
5. Calkins, C.C. and B.F. Sloane. 1995. Mammalian cysteine protease inhibitors: biochemical properties and possible roles in tumor progression. Biol. Chem. Hoppe Seyler 376:71-80.
6. Chirqwin, J.M., A.E. Przybyla, R.J. MacDonald and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294-5299.
7. Clarke, C., J. Titley, S. Davies and M.J. O'Hare. 1994. An immunomagnetic separation method using superparamagnetic (MACS) beads for large-scale purification of human mammary luminal and myoepithelial cells. Epithelial Cell Biol. 3:38-46.
8. Dao, T.L.K., C. Ip and J. Patel. 1980. Serum sialyltransferase and 5′-nucleotidase as reliable biomarkers in women with breast cancer. J. Natl. Cancer Inst. 65:529-534.
9. Foekens, J.A., F. Buessecker, H.A. Peters, U. Krainick, W.L. van-Putten, M.P. Look, J.G. Klign and M.D. Kramer. 1995. Plasminogen activator inhibitor-2: prognostic relevance in 1012 patients with primary breast cancer. Cancer Res. 55:1423-1427.
10. Frigerio, J.M., J.C. Dagorn and J.L. Iovanna. 1995. Cloning, sequencing and expression of the L5, L21, L27a, L28, S5, S9, S10 and S29 human ribosomal protein mRNAs. Biochim. Biophys. Acta 1262:64-68.
11. Harvat, B.L. and A.M. Jetten. 1996. Gamma-interferon induces an irreversible growth arrest in mid-G1 in mammary epithelial cells which correlates with a block in hyperphosphorylation of retinoblastoma. Cell Growth Differ. 7:289-300.
12. Hirschi, K.K., C. Xu, T. Tsukamoto and R. Sager. 1996. Gap junction genes Cx26 and Cx43 individually suppress the cancer phenotype of human mammary carcinoma cells and restore differentiation potential. Cell Growth Differ. 7:861-870.
13. Kester, H.A., B.M. van der Leede, P.T. van der Saag and B. van der Berg. 1997. Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation. J. Biol. Chem. 272:16637-16643.
14. Liang, P. and A.B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971.
15. Linskens, M.H.K., J. Feng, W.H. Andrews, B.E. Enlow, S.M. Saati, L.A. Tonkin, W.D. Funk and B. Villeponteau. 1995. Cataloging altered gene expression in young and senescent cells using enhanced differential display. Nucleic Acids Res. 23:3244-3251.
16. Martin, K., C.-P. Kwan and R. Sager. 1997. A direct-sequencing-based strategy for identifying and cloning cDNAs from differential display gels, p. 77-85. In A.B. Pardee and P. Liang (Eds.), Methods in Molecular Biology. Differential Display: Methods and Applications. Humana Press, Totowa.
17. Martin, K.J. and A.B. Pardee. The principles of differential display. In S. Weissman (Ed.), Methods in Enzymology, cDNA Preparation and Analysis. Academic Press, San Diego (In press).
18. Masiakowski, P., R. Breathnach, J. Bloch, K. Cannon, A. Krust and P. Chambon. 1982. Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line. Nucleic Acids Res. 10:7895-7903.
19. Mou, L., H. Miller, J. Li, E. Wang and L. Chalifour. 1994. Improvements to the differential display method for gene analysis. Biochem. Biophys. Res. Commun. 199:564-569.
20. Poirier, G.M., J. Pyati, J.S. Wan and M.G. Erlander. 1997. Screening differentially expressed cDNA clones obtained by differential display using amplified RNA. Nucleic Acids Res. 25:913-914.
21. Rackley, R.R., T.J. Lewis, E.M. Preston, C.M. Delmoro, E.L. Bradley, M.I. Resnick, T.P. Pretlow and T.G. Pretlow. 1989. 5′-Nucleotidase activity in prostatic carcinoma and benign prostatic hyperplasia. Cancer Res. 49:3702-3707.
22. Sager, R. 1997. Expression genetics in cancer: shifting the focus from DNA to RNA. Proc. Natl. Acad. Sci. USA 94:952-955.
23. Sager, R., S. Sheng, A. Anisowicz, G. Sotiropoulou, Z. Zou, G. Stenman, K. Swisshelm, Z. Chen et. al. 1994. RNA genetics of breast cancer: maspin as paradigm. Cold Spring Harb. Symp. Quant. Biol. 59:537-546.
24. Wang, X. and G.Z. Feuerstein. 1995. Direct sequencing of DNA isolated from mRNA differential display. BioTechniques 18:448-453.
25. Zhang, H., R. Zhang and P. Liang. 1996. Differential screening of gene expression difference enriched by differential display. Nucleic Acids Res. 24:2454-2455.
26. Zhao, S., S.L. Ooi and A.B. Pardee. 1995. New primer strategy improves precision of differential display. BioTechniques 18:842-850.