Optical biopsy of in vivo human skin: Multi-photon excitation microscopy

Lasers in Medical Science

Volumn 13, Issue 3, 1998, Pages 196-203

  Masters, B R ^a   So, P T C ^a,b   Gratton, E ^a  

^a University of Illinois at Urbana Champaign   (United States)

^b Massachusetts Institute of Technology Cambridge   (United States)

Author keywords

Autofluorescence; Multi photon; NADP(H); Redox imaging; Skin imaging; Two photon

Indexed keywords

REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ABSORPTION; ARTICLE; AUTOFLUORESCENCE; CLINICAL TRIAL; EXCITATION; HUMAN; HUMAN TISSUE; IMAGING; INFRARED RADIATION; MICROSCOPY; PHOTON; PRIORITY JOURNAL; SKIN BIOPSY; SPECTROSCOPY; ULTRAVIOLET RADIATION;

EID: 0031792415     PISSN: 02688921     EISSN: None     Source Type: Journal    
DOI: 10.1007/s101030050074     Document Type: Article

References (27)

1. Chance B, Thorell B. Localization and kinetics of reduced pyridine nucleotide in living cells by microfluorometry. J Biol Chem 1959; 234:3044-50
2. Chance B. Pyridine nucleotide as an indicator of the oxygen requirements for energy-linked functions of mitochondria. Circ Res Suppl 1, 1976; 38:I-31-8
3. Chance B, Schoener B, Oshino R et al. Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. J Biol Chem 1979; 254:4764-811
4. Masters BR. In vivo corneal redox fluorometry. In: Masters BR (ed) Noninvasive Diagnostic Techniques in Ophthalmology. New York: Springer-Verlag, 1990: 223-47
5. Masters BR, Chance B. Redox confocal imaging: intrinsic fluorescent probes of cellular metabolism. In: Mason WT (ed) Fluorescent and Luminescent Probes for Biological Activity. London: Academic Press, 1993: 44-57
6. Zhang JC, Savage HZ, Delohery T et al. Innate cellular fluorescence reflects alternations in cellular proliferation. Laser Surg Med 1997; 20:319-31
7. Schantz SP, Savage HZ, Sacks P, Alfano RR. Native cellular fluorescence and its application to cancer prevention. Environ Health Perspect 1997; 105: 941-4
8. Yang Y, Katz A, Celmer EJ et al. Fundamental differences of excitation spectrum between malignant and benign breast tissues. Photochem Photobiol 1997; 66:518-27
9. Cordeiro PG, Kirshner RZ, Hu QY et al. Ultraviolet excitation fluorescence spectroscopy: a non-invasive method for the measurement of redox changes in ischemic myocutaneous flaps. Plast Reconstr Surg 1995; 96:673-80
10. Clark RAF (ed). The Molecular and Cellular Biology of Wound Repair. New York: Plenum Press, 1996
11. Wilson T. Confocal Microscopy. London: Academic Press, 1990
12. Masters BR. Confocal Microscopy: History, Principles, Instruments, and Some Applications to the Living Eye. Comments Mol Cell Biophys 1995; 8:243-71
13. Pawley JB. Handbook of Biological Confocal Microscopy. New York: Plenum Press, 1995
14. Masters BR, Thaer AA. Real-time scanning slit confocal microscopy of the in vivo human cornea. Appl Optics 1994; 33:695-701
15. Petran M, Hadravsky M, Egger MD, Galambos R. Tandem scanning reflected light microscope. J Opt Soc Am 1958; 58:661-4
16. Corcuff P, Bertrand C, Leveque JL. Morphometry of human epidermis in vivo by real-time confocal microscopy. Arch Dermatol Res 1993; 285:475-81
17. Corcuff P, Leveque JL. In vivo vision of human skin with the tandem scanning microscope. Dermatology 1993; 186:50-4
18. Bertrand C, Corcuff P. In vivo spatio-temporal visualization of the human skin by real-time confocal microscopy. Scanning 1994; 16:150-4
19. Masters BR. Three-dimensional confocal microscopy of human skin in vivo: autofluorescence of normal skin. Bioimages 1996; 4:13-19
20. Masters BR, Gonnord G, Corcuff P. Three-dimensional microscopic biopsy of in vivo human skin: a new technique based on a flexible confocal microscope. J Microsc 1997; 185:329-38
21. Rajadhyaksha MM, Grossman M, Esterowitz D et al. In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. J Invest Dermatol 1995; 104:946-52
22. Goeppert-Mayer M. Über Elementarake mit zwei Quantensprungen. Ann Phys (Leipzig) 1931; 5:273-94
23. Denk WJ, Strickler JH, Webb WW. Two-photon laser scanning fluorescence microscopy. Science 1990; 248:73-6
24. Piston DW, Masters BR, Webb WW. Three-dimensionally resolved NAD(P)H cellular metabolic redox imaging of the in situ cornea with two-photon excitation laser scanning microscopy. J Microsc 1995; 178:20-7
25. So PTC, French T, Yu WM et al. Time-resolved fluorescence microscopy using two-photon excitation. Bioimaging 1995; 3:49-63
26. So PTC, French T, Yu WM et al. Two-photon fluorescence microscopy: time-resolved and intensity imaging. In: Wang XF, Herman B (eds) Fluorescence Imaging and Microscopy. Chemical Analysis Series, vol. 137, New York: Wiley, 1996:351-74
27. Koenig K, So PTC, Mantulin WW et al. Two-photon excited lifetime imaging of autofluorescence in cells during UVA and NIR photostress. J Microsc 1996; 183:197-204