Identification of the oxidation states of the active site cysteine in a recombinant protein tyrosine phosphatase by electrospray mass spectrometry using on-line desalting

Rapid Communications in Mass Spectrometry

Volumn 12, Issue 20, 1998, Pages 1457-1462

  DeGnore, Jon P ^a   König, Simone ^a   Barrett, William C ^b   Chock, P Boon ^b   Fales, Henry M ^a,c  

^a NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^b NATIONAL INSTITUTES OF HEALTH   (United States)

^c Building 10   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MODIFICATION; MASS SPECTROMETRY; ONLINE ANALYSIS; OXIDATION; PROTEIN TYROSINE PHOSPHATASE; RECOMBINANT ENZYME; SULFINIC ACID; SULFONIC ACID;

AMINO ACIDS; DESALINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; OXIDATION; PHOSPHATASES;

ACTIVE SITE; CYSTEINE RESIDUES; ELECTROSPRAY MASS SPECTROMETRY; ENZYMATIC ACTIVITIES; HUMAN PROTEINS; LARGE AMOUNTS; OFF-LINE METHODS; ONLINE METHODS; OXIDATION STATE; PROTEIN-TYROSINE PHOSPHATASE;

RECOMBINANT PROTEINS;

EID: 0031791274     PISSN: 09514198     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0231(19981030)12:20<1457::aid-rcm346>3.3.co;2-1     Document Type: Article

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