The greater glycan content of recombinant human thyroid peroxidase of mammalian than of insect cell origin facilitates purification to homogeneity of enzymatically protein remaining soluble at high concentration

Endocrinology

Volumn 139, Issue 3, 1998, Pages 999-1005

  Guo, Jin ^a   McLachlan, Sandra M ^a   Hutchison, Scott ^b   Rapoport, Basil ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b QUEST DIAGNOSTICS NICHOLS INSTITUTE   (United States)

^c SAN FRANCISCO VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCAN; HEMATIN; RECOMBINANT ENZYME; THYROID PEROXIDASE;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; CONCENTRATION (PARAMETERS); DEGLYCOSYLATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME STRUCTURE; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

EID: 0031771379     PISSN: 00137227     EISSN: None     Source Type: Journal    
DOI: 10.1210/endo.139.3.5782     Document Type: Article

References (40)

1. DeGroot LJ, Niepomniszcze H 1977 Biosynthesis of thyroid hormone: basic and clinical aspects. Metabolism 26:665-718
2. McLachlan SM, Rapoport B 1992 The molecular biology of thyroid peroxidase: cloning, expression and role as autoantigen in autoimmune thyroid disease. Endocr Rev 13:192-206
3. Gardas A, Sohi MK, Sutton BJ, McGregor AM, Banga JP 1997 Purification and crystallization of the autoantigen thyroid peroxidase from human Graves' thyroid tissue. Biochem Biophys Res Commun 234:366-370
4. Magnusson RP, Gestautas J, Seto P, Taurog A, Rapoport B 1986 Isolation and characterization of a cDNA clone for porcine thyroid peroxidase. FEBS Lett 208:391-396
5. Magnusson RP, Gestautas J, Taurog A, Rapoport B 1987 Molecular cloning of the structural gene for porcine thyroid peroxidase. J Biol Chem 262:13885-13888
6. Kimura S, Kotani T, McBride OW, Umeki K, Hirai K, Nakayama T, Ohtaki S 1987 Human thyroid peroxidase: complete cDNA and protein sequence, chromosome mapping, and identification of two alternately spliced mRNAs. Proc Natl Acad Sci USA 84:5555-5559
7. Magnusson RP, Chazenbalk GD, Gestautas J, Seto P, Filetti S, DeGroot LJ, Rapoport B 1987 Molecular cloning of the complementary deoxyribonucleic acid for human thyroid peroxidase. Mol Endocrinol 1:856-861
8. Libert F, Ruel J, Ludgate M, Swillens S, Alexander N, Vassart G, Dinsart C 1987 Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J 6:4193-4196
9. Kendler DL, Brennan V, Davies TF, Magnusson RP 1993 Expression of human thyroid peroxidase in insect cells using recombinant baculovirus. Mol Cell Endocrinol 93:199-206
10. Fan J-L, Patibandla SA, Kimura S, Rao T, Desai RK, Seetharamaiah GS, Kurosky A, Prabhakar BS 1996 Purification and characterization of a recombinant human thyroid peroxidase expressed in insect cells. J Autoimmun 9:529-536
11. Seto P, Nagayama Y, Foti D, McLachlan SM, Rapoport B 1993 Autoantibodies in the sera of patients with autoimmune thyroid disease recognize a secreted form of thyroid peroxidase generated in a baculovirus system. Mol Cell Endocrinol 94:R5-R8
12. Grennan Jones F, Wolstenholme A, Fowler S, Smith S, Ziemnicka K, Bradbury J, Furmaniak J, Rees Smith B 1996 High-level expression of recombinant immunoreactive thyroid peroxidase in the High Five insect cell line. J Mol Endocrinol 17:165-174
13. Foti D, Kaufman KD, Chazenbalk G, Rapoport B 1990 Generation of a biologically-active, secreted form of human thyroid peroxidase by site-directed mutagenesis. Mol Endocrinol 4:786-791
14. Kaufman KD, Foti D, Seto P, Rapoport B 1991 Overexpression of an immunologically-intact, secreted form of human thyroid peroxidase in eukaryotic cells. Mol Cell Endocrinol 78:107-114
15. Bradford MM 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
16. Chazenbalk GD, Portolano S, Russo D, Hutchison JS, Rapoport B, McLachlan SM 1993 Human organ-specific autoimmune disease: molecular cloning and expression of an autoantibody gene repertoire for a major autoantigen reveals an antigenic dominant region and restricted immunoglobulin gene usage in the target organ. J Clin Invest 92:62-74
17. Chance B, Maehly AC 1955 Assay of catalases and peroxidases. Methods Enzymol 2:764-775
18. Rawitch AB, Taurog A, Chernoff SB, Dorris ML 1979 Hog thyroid peroxidase: physical, chemical and catalytic properties of the highly purified enzyme. Arch Biochem Biophys 194:244-257
19. Laemmli UK 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
20. McLachlan SM, Rapoport B 1995 Genetic and epitopic analysis of thyroid peroxidase (TPO) autoantibodies: markers of the human thyroid autoimmune response. Clin Exp Immunol 101:200-206
21. Rapoport B, Portolano S, McLachlan SM 1995 Combinatorial immunoglobulin gene libraries: new insights into human organ-specific autoantibodies. Immunol Today 16:43-49
22. Kaufman KD, Rapoport B, Seto P, Chazenbalk GD, Magnusson RP 1989 Generation of recombinant, enzymatically-active, human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto's thyroiditis. J Clin Invest 84:394-403
23. Alexander NM, Burrow GN 1970 Thyroxine biosynthesis in human goitrous cretinism. J Clin Endocrinol Metab 30:308-315
24. Niepomniszcze H, DeGroot LJ, Hagen GA 1972 Abnormal thyroid peroxidase causing iodide organification defect. J Clin Endocrinol Metab 34:607-616
25. Hosoya T, Morrison M 1967 The isolation and purification of thyroid peroxidase. J Biol Chem 242:2828-2836
26. Taurog A, Dorris ML, Yokoyama N, Slaughter C 1990 Purification and characterization of a large, tryptic fragment of human thyroid peroxidase with high catalytic activity. Arch Biochem Biophys 278:333-341
27. Ohtaki S, Kotani T, Nakamura Y 1986 Characterization of human thyroid peroxidase purified by monoclonal antibody-assisted chromatography. J Clin Endocrinol Metab 63:570-576
28. Czarnocka B, Ruf J, Ferrand M, Carayon P, Lissitzky S 1985 Purification of the human thyroid peroxidase and identification as the microsomal antigen in thyroid diseases. FEBS Lett 109:147-152
29. Yokoyama N, Taurog A 1988 Porcine thyroid peroxidase: relationship between the native enzyme and an active, highly purified tryptic fragment. Mol Endocrinol 2:838-844
30. Fenna RE, Zeng J, Davey C 1995 Structure of the green heme in myeloperoxidase. Arch Biochem Biophys 316:653-656
31. Andersson LA, Bylkas SA, Wilson AE 1996 Spectral analysis of lactoperoxidase. Evidence for a common heme in mammalian peroxidases. J Biol Chem 271:3406-3412
32. Rae TD, Goff HM 1996 Lactoperoxidase heme structure characterized by paramagnetic proton NM spectroscopy. J Am Chem Soc 118:2103-2121
33. DePillis GD, Ozaki S, Kuo JM, Maltby DA, Ortiz de Montellano PR 1997 Autocatalytic processing of heme by lactoperoxidase produces the native protein-bound prosthetic group. J Biol Chem 272:8857-8860
34. Jacquet A, Deby C, Mathy M, Moguilevsky N, Deby-Dupont G, Thirion A, Goormaghtigh E, Garcia-Quintana L, Bollen A, Pincemail J 1991 Spectral and enzymatic properties of human recombinant myeloperoxidase: comparison with the mature enzyme. Arch Biochem Biophys 291:132-138
35. Foti D, Rapoport B 1990 Carbohydrate moieties in recombinant human thyroid peroxidase: role in recognition by antithyroid peroxidase antibodies in Hashimoto's thyroiditis. Endocrinology 126:2983-2988
36. Moura EG, Pazos-Moura CC, Yokoyama N, Dorris ML, Taurog A 1991 Enzymatic deglycosylation of porcine thyroid peroxidase: effects on catalytic activity and immunoreactivity. Acta Endocrinol (Copenh) 124:107-114
37. Kiso Y, Furmaniak J, Morteo C, Rees Smith B 1992 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyroglobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. Autoimmunity 12:259-269
38. Giraud A, Franc J-L, Long Y, Ruf J 1992 Effects of deglycosylation of human thyroperoxidase on its enzymatic activity and immunoreactivity. J Endocrinol 132:317-323
39. Chazenbalk GD, Rapoport B 1995 Expression of the extracellular region of the thyrotropin receptor in a baculovirus vector using a promoter active earlier than the polyhedrin promoter: implications for the expression of functional, highly glycosylated proteins. J Biol Chem 270:1543-1549
40. Rapoport B, McLachlan SM, Kakinuma A, Chazenbalk GD 1996 Critical relationship between autoantibody recognition and TSH receptor maturation as reflected in the acquisition of mature carbohydrate. J Clin Endocrinol Metab 81:2525-2533