High-level expression of recombinant immunoreactive thyroid peroxidase in the High Five insect cell line

Journal of Molecular Endocrinology

Volumn 17, Issue 2, 1996, Pages 165-174

  Grennan Jones, F ^a   Wolstenholme, A ^b   Fowler, S ^b   Smith, S ^a   Ziemnicka, K ^a   Bradbury, J ^a   Furmaniak, J ^a,c   Rees Smith, B ^a,c  

^a RSR Limited   (United Kingdom)

^b UNIVERSITY OF BATH   (United Kingdom)

^c CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

THYROID PEROXIDASE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HASHIMOTO DISEASE; INSECT; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL;

EID: 0029862281     PISSN: 09525041     EISSN: None     Source Type: Journal    
DOI: 10.1677/jme.0.0170165     Document Type: Article

References (39)

1. Barnes HJ, Jenkins CM & Waterman MR 1994 Baculovirus expression of bovine cytochrome P450c17 in Sf9 cells and comparison with expression in yeast, mammalian cells, and E coli. Archives of Biochemistry and Biophysics 315 489-494.
2. Bayer EA & Wilchek M 1990 Protein biotinylation. Methods in Enzymology 184 138-160.
3. Beever K, Bradbury J, Phillips D, McLachlan SM, Pegg C, Goral A, Overbech W, Felfel G & Rees Smith B 1989 Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase. Clinical Chemistry 35 1949-1954
4. Birk HW & Koepsell H 1987 Reaction of monoclonal antibodies with plasma membrane proteins after binding on nitrocellulose renaturation of antigenic sites and reduction of nonspecific antibody binding. Analytical Biochemistry 164 12-22
5. Bishop DHL & Possee RD 1990 Baculovirus expression vectors Advances in Gene Technology 1 55-72.
6. Chazenbalk GD & Rapoport B 1995 Expression of the extracellular domain of the thyrotropin receptor in the baculovirus system using a promoter active earlier than the polyhedrin promoter Journal of Biological Chemistry 270 1543-1549.
7. Chung BC, Hu MC, Guzov VM & Wu DA 1995 Structure and expression of the CYP21 (P4SOc21, steroid 21-hydroxylase) gene with respect to its deficiency. Endocrine Research 21 343-352.
8. Czarnocka B, Ruf J, Ferrand M, Carayon P & Lissitzky S 1985 Purification of the human thyroid peroxidase and its identification as the microsomal antigen involved in autoimmune thyroid diseases. FEBS Letters 190 147-152.
9. Finke R, Seto P & Rapoport B 1990 Evidence of the highly conformalional nature of the epitope(s) on human thyroid peroxidase that are recognised by sera from patients with Hashimoto's thyroiditis Journal of Clinical Endocrinology and Metabolism 71 53-59.
10. Foti D & Rapoport B 1990 Carbohydrate moieties in recombinant human thyroid peroxidase. Role in recognition by antithyroid peroxidase antibodies in Hashimoto's thyroiditis Endocrinology 126 2983-2988.
11. Furmaniak J & Rees Smith B 1990 The structure of thyroid autoantigens. Autoimmunity 7 63-80
12. 131I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89 114-123.
13. Hartmann C & Ortiz de Montellano PR 1992 Baculovirus expression and characterization of catalytically active horseradish peroxidase. Archives of Biochemistry and Biophysics 297 61-72
14. Haubruck H, Mauch L, Cook NJ, Steffens U, Hunt N, Berthold H, Niemann H, Wirbelauer C & Northemann W 1993 Expression of recombinant human thyroid peroxidase by the baculovirus system and its use in ELISA screening for diagnosis of autoimmune thyroid disease. Autoimmunity 15 275-284.
15. Hosoya T & Morrison M 1967 The isolation and purification of thyroid peroxidase. Journal of Biological Chemistry 242 2828-2836
16. Kaufman KD, Rapoport B, Seto P, Chazenbalk GD & Magnusson RP 1989 Generation of recombinant, enzymatically active human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto's thyroiditis. Journal of Clinical Investigation 84 394-403.
17. Kaufman KD, Filetti S, Seto P & Rapoport B 1990 Recombinant human thyroid peroxidase generated in eukaryotic cells' a source of specific antigen for the immunological assay of antimicrosomal antibodies in the sera of patients with autoimmune thyroid disease. 'Journal of Clinical Endocrinology and Metabolism 70 724-728.
18. Kaufman KD, Foti D, Seto P & Rapoport B 1991 Overexpression of an immunologically-intact, secreted form of human thyroid peroxidase in eukaryotic cells. Molecular and Cellular Endocrinology 78 107-114
19. Kendler DL, Brennan V, Davies TF & Magnusson RP 1993 Expression of human thyroid peroxidase in insect cells using, recombinant baculovirus. Molecular and Cellular Endocrinology 93 199-206.
20. King LA & Possee RD 1992 The Baculovirus Expression System; A Laboratory Guide. London: Chapman and Hall.
21. Kiso Y, Furmaniak J, Morteo C & Rees Smith B 1992 Analysis of carbohydrate residues on human thyroid peroxidase (TPO) and thyrogiobulin (Tg) and effects of deglycosylation, reduction and unfolding on autoantibody binding. Autoimmunity 12 259-269.
22. Libert F, Ludgate M, Dinsart C & Vassart G 1991 Thyroperoxidase, but not the thyrotropin receptor, contains sequential epitopes recognized by autoantibodies in recombinant peptides expressed m the pUEX vector Journal of Clinical Endocrinology and Metabolism 73 857-860.
23. Licari P & Bailey JE 1991 Factors influencing recombinant protein yields in an insect cell-baculovirus expression system: Multiplicity of infection and intracellular protein degradation. Biotechnology and Bioengineering 37 238-246.
24. Luckow VA & Summers MD 1988 Trends in the development of baculovirus expression vectors. Bio/Technology 6 47-55.
25. Ludgate M, Mariotti S, Libert F, Dinsart C, Piccolo P, Santini F, Ruf J, Pinchera A & Vassart G 1989 Antibodies to human thyroid peroxidase in autoimmune thyroid disease: Studies with a cloned recombinant complementary deoxyribonucleic acid epitope. Journal of Clinical Endocrinology and Metabolism 68 1091-1096.
26. Nakajima Y, Howells RD, Pegg C, Davies Jones E & Rees Smith B 1987 Structure-activity analysis of microsomal antigen/thyroid peroxidase. Molecular and Cellular Endocrinology 53 15-23.
27. Ohtaki S, Kotani T & Nakamura Y 1986 Characterization of human thyroid peroxidase purified by monoclonal antibody-assisted chromatography. Journal of Clinical Endocrinology and Metabolism 63 570-576.
28. O'Reilly DR, Miller LK & Luckow VA 1992 Baculovirus Expression Vectors, A Laboratory Manual. New York: WH Freeman and Company.
29. Parker EM, Kameyama K, Higashijima T & Ross EM 1991 Reconstitutively active G protein-coupled receptors purified from baculovirus-infected insect cells. Journal of Biological Chemistry 266 519-527.
30. Paul JI, Tavare J, Denton RM & Steiner DF 1990 Baculovirus-directed expression of the human insulin receptor and an insulin-binding ectodomain. Journal of Biological Chemistry 265 13074-13083.
31. Paulson JC 1989 Glycoproteins: what are the sugar chains for? Trends in Biochemical Sciences 14 272-279.
32. Premawardhana LDKE, Kiso Y, Phillips DI, Morteo C, Furmaniak J & Rees Smith B 1993 Is TPO detectable in the circulation? Thyroid 3 225-228.
33. Rawitch AB, Pollock G, Yang S-X & Taurog A 1992 Thyroid peroxidase glycosylation: Nature of the N-linked oligosaccharide units in porcine thyroid peroxidase. Archives of Biochemistry and Biophysics 297 321-327.
34. Sambrook J, Fritsch EF & Maniatis T 1989 Molecular Cloning: A Laboratory Manual, edn 2. Cold Spring Harbor: Cold Spring Harbor Laboratory Press.
35. Seo HG, Fujii J, Soejima H, Niikawa N & Taniguchi N 1995 Heme requirement for production of active endothelial nitric oxide synthase in baculovirus-infected insect cells. Biochemical and Biophysical Research Communications 208 10-18.
36. Seto P, Nagayama Y, Foti D, McLachlan SM & Rapoport B 1993 Autoantibodies in the sera of patients with autoimmune thyroid disease recognize a secreted form of human thyroid peroxidase generated in a baculovirus system. Molecular and Cellular Endocrinology 94 R5-R8.
37. Taurog A, Dorris ML, Yokoyama X & Slaughter C 1990 Purification and characterization of a large, tryptic fragment of human thyroid peroxidase with high catalytic activity. Archives of Biochemistry and Biophysics 278 333-341.
38. Tonacchera M, Cetani F, Costagliola S, Alcalde L, Uibo R, Vassart G & Ludgate M 1995 Mapping thyroid peroxidase epitopes using recombinant protein fragments European Journal of Endocrinology 132 53-61.
39. Wedlock N, Furmaniak J, Fowler S, Kiso Y, Bednarek J, Baumann-Antczak A, Morteo C, Sudbery P, Hinchcliff A & Rees Smith B 1993 Expression of human thyroid peroxidase in the yeasts Saccharomyces cere-risiae and Hansenula polymorpha. Journal of Molecular Endocrinology 10 325-336.