RNA chip: Quality assessment of RNA by microchannel linear gel electrophoresis in injection-molded plastic chips

Clinical Chemistry

Volumn 44, Issue 11, 1998, Pages 2249-2255

  Ogura, Mieko ^a   Agata, Yoichi ^b   Watanabe, Kenji ^b   Mccormick, Randy M ^c   Hamaguchi, Yohei ^d   Aso, Yoshimasa ^d   Mitsuhashi, Masato ^a,d,e  

^a Hitachi Chemical Research Center   (United States)

^b High Performance Materials Department   (Japan)

^c Soane Technologies Inc   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RIBOSOME RNA; RNA 18S; RNA 28S;

ACCURACY; ARTICLE; CONTROLLED STUDY; DISPOSABLE EQUIPMENT; HUMAN; HUMAN CELL; MICROANALYSIS; REACTION TIME; RNA ANALYSIS;

EID: 0031762736     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Bortolin S, Christopoulos TK. Quantitative RT-PCR combined with time-resolved fluorometry for determination of BCR-ABL mRNA. Clin Chem 1996;42:1924-9.
2. Lundeberg J, Uhlen M. Assessment of sequence-based p53gene analysis in human breast cancer: messenger RNA in comparison with genomic DNA targets. Clin Chem 1998;44:455-62.
3. Gala J-L, Heusterspreute M, Loric S, Hanon F, Tombal B, Van Cangh P, et al. Expression of prostate-specific antigen and prostate-specific membrane antigen transcripts in blood cells: implications for the detection of hematogenous prostate cells and standardization. Clin Chem 1998;44:472-81.
4. Miura Y, Ichikawa Y, Ishikawa T, Ogura M, de Fries R, Shimada H, Mitsuhashi M. Fluorometric determination of total mRNA with oligo(dT) immobilized on microtiter plates. Clin Chem 1996;42: 1758-64.
5. Tominaga K, Miura Y, Arakawa T, Kobayashi K, Mitsuhashi M. Colorimetric ELISA measurement of specific mRNA on immobilized-oligonucleotide-coated microtiter plates by reverse transcription with biotinylated mononucleotides. Clin Chem 1996;42: 1750-7.
6. Neumaier M, Braun A, Wagner C. Fundamentals of quality assessment of molecular amplification methods in clinical diagnostics. Clin Chem 1998;44:12-26.
7. Taguchi Y. Measurement of microtopography: 3D measuring method by SEM. Jpn J Tribol 1990;351-7.
8. McCormick RM, Nelson RJ, Alonso-Amigo MG, Benvegnu DJ, Hooper HH. Microchannel electrophoretic separations of DNA in injection-molded plastic substrates. Anal Chem 1997;69: 2626-30.
9. Sambrook J, Fritsch EF, Maniatis T. Extraction, purification and analysis of messenger RNA from eukaryotic cells. In: Molecular cloning, a laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989;7.1-7.87.
10. Skeidsvoll J, Ueland PM. Analysis of RNA by capillary electrophoresis. Electrophoresis 1996;17:1512-7.
11. Woolley AT, Mathies RA. Ultra-high-speed DNA fragment separations using microfabricated capillary array electrophoresis chips. Proc Natl Acad Sci U S A 1994;91:11348-52.
12. Woolley AT, Mathies RA. Ultra-high-speed DNA sequencing using capillary electrophoresis chips. Anal Chem 1995;67: 3676-80.
13. Schmalzing D, Koutny L, Adourian A, Belgrader P, Matsudaira P, Ehrlich D. DNA typing in thirty seconds with a microfabricated device. Proc Natl Acad Sci U S A 1997;94:10273-8.