Localization of a voltage gate in Connexin46 gap junction hemichannels

Biophysical Journal

Volumn 75, Issue 5, 1998, Pages 2323-2331

  Pfahnl, Arnold ^a   Dahl, Gerhard ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CONNEXIN 46; CYSTEINE; GAP JUNCTION PROTEIN; NALPHA (4 MALEIMIDOBUTYRYL)BIOCYTIN; THIOL REAGENT; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; CHANNEL GATING; GAP JUNCTION; MEMBRANE CONDUCTANCE; MEMBRANE POTENTIAL; NONHUMAN; OOCYTE; PROTEIN BINDING; PROTEIN LOCALIZATION; SITE DIRECTED MUTAGENESIS; VOLTAGE CLAMP;

EID: 0031755597     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(98)77676-3     Document Type: Article

References (33)

1. Armstrong, C. M. 1971. Interaction of tetraethylammonium ion derivatives with the potassium channels of giant axons. J. Gen. Physiol. 58: 413-437.
2. Barrio, L. C., T. Suchyna, T. Bargiello, L. X. Xu, R. S. Roginski, M. V. Bennett, and B. J. Nicholson. 1991. Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage. Proc. Natl. Acad. Sci. USA. 88:8410-8414.
3. Baud, C., R. T. Kado, and K. Marcher. 1982. Sodium channels induced by depolarization of the Xenopus laevis oocyte. Proc. Natl. Acad. Sci. USA. 79:3188-3192.
4. Bennett, M. V., L. C. Barrio, T. A. Bargiello, D. C. Spray, E. Hertzberg, and J. C. Saez. 1991. Gap junctions: new tools, new answers, new questions. Neuron. 6:305-320.
5. Beyer, E. C., D. L. Paul, and D. A. Goodenough. 1990. Connexin family of gap junction proteins. J. Membr. Biol. 116:187-194.
6. Bukauskas, F. F., C. Elfgang, K. Willecke, and R. Weingart. 1995. Heterotypic gap junction channels (connexin26-connexin32) violate the paradigm of unitary conductance. Pflugers Arch. 429:870-872.
7. Dahl, G. 1992. The Xenopus oocyte cell-cell channel assay for functional analysis of gap junction proteins. In Cell-Cell Interactions. A Practical Approach. IRL, Oxford. 143-165.
8. Ebihara, L., V. M. Berthoud, and E. C. Beyer. 1995. Distinct behavior of connexin56 and connexin46 gap junctional channels can be predicted from the behavior of their hemi-gap-junctional channels. Biophys. J. 68:1796-1803.
9. Ebihara, L., and E. Steiner. 1993. Properties of a nonjunctional current expressed from a rat connexin46 cDNA in Xenopus oocytes. J. Gen. Physiol. 102:59-74.
10. Ekvitorin, J. F., G. Calero, G. E. Morley, W. Coombs, S. M. Taffet, and M. Delmar. 1996. pH regulation of connexin43 - molecular analysis of the gating particle. Biophys. J. 71:1273-1284.
11. Gupta, V. K., V. M. Berthoud, N. Atal, J. A. Jarillo, L. C. Barrio, and E. C. Beyer. 1994. Bovine connexin44, a lens gap junction protein: molecular cloning, immunologic characterization, and functional expression. Invest. Ophthalmol. Vis. Sci. 35:3747-3758.
12. Hamill, O. P., A. Marty, E. Neher, B. Sakmann, and F. J. Sigworth. 1981. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch. 391:85-100.
13. Hodgkin, A. L., and A. F. Huxley. 1952d. A quantitative description of membrane current and its application to conduction and excitation in nerve. J. Physiol. (Lond.). 116:507-544.
14. + channels: evidence for a trap door mechanism of activation gating. J. Gen. Physiol. 109:527-535.
15. Hoshi, T., W. N. Zagotta, and R. W. Aldrich. 1990. Biophysical and molecular mechanisms of Shaker potassium channel inactivation. Science. 250:533-538.
16. Kelsell, D. P., J. Dunlop, H. P. Stevens, N. J. Lench, J. N. Liang, G. Parry, R. F. Mueller, and I. M. Leigh. 1997. Connexin 26 mutations in hereditary non-syndromic sensorineural deafness. Nature. 387:80-83.
17. + channel. Neuron. 19:175-184.
18. Loewenstein, W. R. 1981. Junctional intercellular communication: the cell-to-cell membrane channel. Phys. Rev. 61:829-912.
19. Oh, S., Y. Ri, M. V. Bennett, E. B. Trexler, V. K. Verselis, and T. A. Bargiello. 1997. Changes in permeability caused by connexin 32 mutations underlie X-linked Charcot-Marie-Tooth disease. Neuron. 19: 927-938.
20. Paul, D. L., L. Ebihara, L. J. Takemoto, K. I. Swenson, and D. A. Goodenough. 1991. Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes. J. Cell Biol. 115:1077-1089.
21. Pfahnl, A., X. W. Zhou, R. Werner, and G. Dahl. 1997. A chimeric connexin forming gap junction hemichannels. Pflugers Arch. 433: 773-779.
22. Rubin, J. B., V. K. Verselis, M. V. Bennett, and T. A. Bargiello. 1992. Molecular analysis of voltage dependence of heterotypic gap junctions formed by connexins 26 and 32. Biophys. J. 62:183-193.
23. Tan, C. C., P. J. Ainsworth, A. F. Hahn, and P. M. MacLeod. 1996. Novel mutations in the connexin 32 gene associated with X-linked Charcot-Marie tooth disease. Hum. Mutat. 7:167-71.
24. Trexler, E. B., M. V. Bennett, T. A. Bargiello, and V. K. Verselis. 1996. Voltage gating and permeation in a gap junction hemichannel. Proc. Natl. Acad. Sci. USA. 93:5836-5841.
25. Unwin, N. 1989. The structure of ion channels in membranes of excitable cells. Neuron 3:665-676.
26. Unwin, P. N., and P. D. Ennis. 1984. Two configurations of a channel-forming membrane protein. Nature. 307:609-613.
27. Unwin, P. N., and G. Zampighi. 1980. Structure of the junction between communicating cells. Nature. 283:545-549.
28. Veenstra, R. D. 1996. Size and selectivity of gap junction channels formed from different connexins. J. Bioenerg. Biomembr. 28:327-337.
29. Verselis, V. K., C. S. Ginter, and T. A. Bargiello. 1994. Opposite voltage gating polarities of two closely related connexins. Nature. 368:348-351.
30. Werner, R., E. Levine, C. Rabadan-Diehl, and G. Dahl. 1989. Formation of hybrid cell-cell channels. Proc. Natl. Acad. Sci. USA. 86:5380-5384.
31. White, T. W., R. Bruzzone, D. A. Goodenough, and D. L. Paul. 1994. Voltage gating of connexins [letter]. Nature. 371:208-209.
32. Wilders, R., and H. J. Jongsma. 1992. Limitations of the dual voltage clamp method in assaying conductance and kinetics of gap junction channels. Biophys. J. 63:942-953.
33. Zhou, X. W., A. Pfahnl, R. Werner, A. Hudder, A. Llanes, A. Luebke, and G. Dahl. 1997. Identification of a pore lining segment in gap junction hemichannels. Biophys. J. 72:1946-1953.