Phospholipid rearrangement of apoptotic membrane does not depend on nuclear activity

Histochemistry and Cell Biology

Volumn 110, Issue 5, 1998, Pages 467-476

  Renò, Filippo ^a   Burattini, Sabrina ^a   Rossi, Stefano ^a   Luchetti, Francesca ^a   Columbaro, Marta ^b   Santi, Spartaco ^c   Papa, Stefano ^a   Falcieri, Elisabetta ^a,c  

^a UNIVERSITY OF URBINO   (Italy)

^b RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^c CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

APOPTOSIS; ARTICLE; CELL STRAIN HL 60; CELL ULTRASTRUCTURE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; DNA DEGRADATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; TRANSMISSION ELECTRON MICROSCOPY;

APOPTOSIS; CELL MEMBRANE; FLUORESCENCE; HL-60 CELLS; HUMANS; PHOSPHOLIPIDS;

EID: 0031751612     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180050308     Document Type: Article

References (38)

1. ++ concentration and DNA fragmentation. Cancer Res 52:1342-1346
2. Bevers EM, Tilly RHJ, Senden JMG, Comfurius P, Zwaal RFA (1989) Exposure of endogenous phosphatidylserine at the outer surface of stimulated platelets is reversed by restoration of aminophospholipid translocase activity. Biochemistry 28: 2382-2387
3. Boe R, Gjertsen BT, Vintermyr OK, Houge G, Lanotte M, Doskeland SO (1991) The protein phosphatase inhibitor okadaic acid induces morphological changes typical of apoptosis in mammalian cells. Exp Cell Res 195:237-246
4. Boersma AWM, Nooter K, Oostrum RG, Stoter G (1996) Quantification of apoptotic cells with fluorescein isothiocyanate-labeled annexin V in Chinese hamster ovary cell cultures treated with cisplatin. Cytometry 24:123-130
5. Cohen JJ (1991) Programmed cell death in the immune system. Adv Immunol 50:890-893
6. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hots MA, Lassota P, Traganos F (1992) Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808
7. Fadok VA, Voelker DR, Campbell PA, Cohen JJ, Bratton DL, Henson PM (1992) Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J Immunol 148:2207-2216
8. Falcieri E, Martelli AM, Bareggi R, Cocco L (1993) The protein kinase inhibitor staurosporine induces morphological changes typical of apoptosis in Molt-4 cells without concomitant DNA fragmentation. Biochem Biophys Res Commun 193:19-25
9. Falcieri E, Zamai L, Santi S, Cinti C, Gobbi P, Bosco D, Cataldi A, Betts C, Vitale M (1994a) The behaviour of nuclear domains in the course of apoptosis. Histochemistry 102:221-231
10. Falcieri E, Gobbi P, Cataldi A, Zamai L, Faenza I, Vitale M (1994b) Nuclear pores in the apoptotic cell. Histochem J 26: 754-763
11. Falcieri E, Cataldi A, Di Baldassarre A, Robuffo I, Miscia S (1996) Morphological patterns and DNA polymerase regulation in apoptotic HL60 cells. Cell Struct Funct 21:213-220
12. Goldstein P, Ojcius DM, Young JDE (1991) Cell death mechanism and the immune system. Immunol Rev 121:29-65
13. Homburg CHE, De Haas M, Von Dem Borne AEGK, Verhoeven AJ, Reutelingsperger CPM, Roos D (1995) Human neutrophils lose their surface FcγRIII and acquire annexin V binding sites during apoptosis in vitro. Blood 85:532-540
14. Jacobson MJ, Burne JF, Raff MC (1994) Programmed cell death and Bcl-2 protection in the absence of a nucleus. EMBO J 13:1899-1910
15. Kaufmann SH, Desnoyers S, Ottaviano Y, Davidson NE, Poirer GG (1993) Specific proteolytic cleavage of poly(ADP-ribose) polymerase: an early marker of chemotherapy-induced apoptosis. Cancer Res 53:3976-3985
16. Koopman G, Reutelingsperger CPM, Kuijten GAM, Keehen RMJ, Pals ST, Van Oers MHJ (1994) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415-1420
17. Koury MJ (1992) Programmed cell death (apoptosis) in hematopoiesis. Exp Hematol 20:391-394
18. Martin SJ, Cotter TG (1991) Ultraviolet B irradiation of human leukaemia HL60 cells in vitro induces apoptosis. J Radiat Biol 59:1001-1016
19. Martin SJ, O'Bren GA, Nishioka WK, McGahon AJ, Mahboudi A, Saido TC, Green DR (1995a) Proteolysis of Fodrin (non-erythroid spectrin) during apoptosis. J Biol Chem 270:6425-6428
20. Martin SJ, Reutelingsperger CMP, McGahon AJ, Rader J, Van Schie RCCA, LaFace DM, Green DR (1995b) Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus. Inhibition by overexpression of Bcl-2 and Abl. J Exp Med 182:1545-1557
21. Mower DA, Peckham DW, Illera VA, Fishbaugh JK, Stunz LL, Ashman RF (1994) Decreased membrane phospholipid packing in mature mouse B cell apoptosis. J Immunol 152: 4832-4842
22. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C (1991) A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods 139:271-279
23. Oberhammer F, Wilson JW, Dive C, Morris ID, Hickman JA, Wakeling AE, Walker PR, Sikorska M (1993) Apoptotic death in epithelial cells: cleavage of DNA to 300 and/or 50 kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J 12:3679-3684
24. Op Den Kamp JAF (1979) Lipid asymmetry in membranes. Annu Rev Biochem 48:47-71
25. Peitsch C, Muller C, Tschopp J (1993) DNA fragmentation during apoptosis is caused by frequent single strand cuts. Nucleic Acids Res 21:4206-4209
26. Savill JS, Henson PM, Haslett C (1989) Phagocytosis of agent human neutrophils by macrophages is mediated by a novel "charge sensitive" recognition mechanism. J Clin Invest 84: 1518-1527
27. Sen S, D'Incalci M (1992) Biochemical events and relevance to cancer chemotherapy. FEBS Lett 307:122-127
28. Sorenson CM, Barry MA, Eastman A (1990) Analysis of events associated with cell cycle arrest at G2 phase and cell death induced by cisplatin. J Natl Cancer Inst 82:749-755
29. Stuppia L, Gobbi P, Zamai L, Palka G, Vitale M, Falcieri E (1996) Morphological and functional study of apoptotic cell chromatin. Cell Death Differ 3:397-405
30. Van Engeland M, Ramaekers FCS, Shutte B, Reutelingsperger CPM (1996) A novel assay to measure loss of plasma membrane asymmetry during apoptosis of adherent cells in culture. Cytometry 24:131-139
31. Verhoven B, Schlegel AR, Williamson P (1995) Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes. J Exp Med 182:1597-1601
32. Vermes I, Haanem C, Steffens-Nakken H, Reutelingsperger C (1995) A novel assay for apoptosis flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled annexin V J Immunol Methods 184:39-51
33. Vitale M, Zamai L, Mazzotti G, Cataldi A, Falcieri E (1993) Differential kinetics of propidium iodide uptake in apoptotic and necrotic thymocytes. Histochemistry 100:223-229
34. Walker PR, Pandly S, Sikorska M (1995) Degradation of chromatin in apoptotic cells. Cell Death Differ 2:97-104
35. Williams GT, Smith CA (1993) Molecular regulation of apoptosis: genetic controls on cell death. Cell 74:777-779
36. Wyllie AH, Kerr JFR, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 68:251-306
37. Zamai L, Falcieri E, Zauli G, Cataldi A, Vitale M (1993) Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 14:891-897
38. Zamai L, Falcieri E, Marhefka G, Vitale M (1996) Supravital exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentation. Cytometry 23:303-311