Supravital exposure to propidium iodide identifies apoptotic cells in the absence of nucleosomal DNA fragmentation

Cytometry

Volumn 23, Issue 4, 1996, Pages 303-311

  Zamai, Loris ^a,e   Falcieri, Elisabetta ^b,c   Marhefka, Gregary ^a   Vitale, Marco ^c,d,f  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF CHIETI   (Italy)

^c Ist Citomorfologia Normale P   (Italy)

^d UNIVERSITY OF BRESCIA   (Italy)

^e THOMAS JEFFERSON UNIVERSITY HOSPITAL   (United States)

^f Dipartimento di Scienze Biomediche e Biotecnologie   (Italy)

Author keywords

Apoptosis; Cell membrane; Flow cytometry; Necrosis

Indexed keywords

CAMPTOTHECIN; DNA FRAGMENT; PROPIDIUM IODIDE; STAUROSPORINE; DNA;

APOPTOSIS; ARTICLE; CELL MEMBRANE; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; HYPERTHERMIA; LEUKEMIA CELL; NUCLEOSOME; PRIORITY JOURNAL; EXPOSURE;

ANIMALS; APOPTOSIS; COLORING AGENTS; DNA DAMAGE; FLOW CYTOMETRY; HL-60 CELLS; HUMANS; MICE; NUCLEOSOMES; PROPIDIUM; TUMOR CELLS, CULTURED;

EID: 0029986785     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(19960401)23:4<303::AID-CYTO6>3.0.CO;2-H     Document Type: Article

References (34)

1. Afanasyev VN, Korol BA, Matylevich NP, Pechatnikov VA, Umansky SR: The use of flow cytometry for the investigation of cell death. Cytometry 14:603-609, 1993.
2. Arenas MJ, Morris RG, Wyllie AH: Apoptosis: The role of the endonuclease. Am J Path 136:593-608, 1990.
3. Barbieri D, Troiano L, Grassilli E, Agnesini C, Cristofelo EA, Monti D, Capri M, Cossarizza A, Franceschi C: Inhibition of apoptosis by zinc: A reappraisal. Biochem Biophys Res Comm 187:1256-1261, 1992.
4. Bruno S, Ardelt B, Skierski JS, Traganos F, Darzynkiewicz Z: Different effects of staurosporine, an inhibitor of protein kinases, on the cell cycle and chromatin structure of normal and leukemic lymphocytes Cancer Res 51:470-473, 1992.
5. Bruno S, Del Bino G, Lassota P, Giaretti W, Darzynkiewicz Z: Inhibitors of proteases prevent endonucleolysis accompanying apoptotic death of HL-60 leukemic cells and normal thymocytes. Leukemia 6:113-1120, 1992.
6. Catchpoole DR, Stewart BW: Etoposide-induced cytotoxicity in two human T-cell leukemic lines: Delayed loss of membrane permeability rather than DNA fragmentation as an indicator of programmed cell death. Cancer Res 53:4287-4296, 1993.
7. Chapman RS, Chresta CM, Herberg AH, Beere HM, Heer S, Whetton AD, Hickman JA, Dive C: Further characterisation of the in situ terminal deoxynucleotidil transferase (TdT) assay for the flow cytometric analysis of apoptosis in drug resistant and drug sensitive leukaemic cells. Cytometry 20:245-256, 1995.
8. Collins RJ, Harmon BV, Gobé GC, Kerr JFR: Internucleosomal DNA cleavage should not be the sole criterion for identifying apoptosis. Int Radiat Biol 61(4):451-453, 1992.
9. Curnow SJ, Barad M, Brun-Roubereau N, Schmitt-Verhulst AM: Flow-cytometric analysis of apoptotic and nonapoptotic T-cell receptor-transgenic thymocytes following in vitro presentation of antigen. Cytometry 16:41-48, 1994.
10. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hots MA, Lassota P, Traganos F: Features of apoptotic cells measured by flow cytometry. Cytometry 13:795-808, 1992.
11. De La Torre J, Summer AT, Gosalvez J, Stuppia L: The distribution of genes on human chromosomes as studied by in situ nick translation. Genome 35:890-896, 1992.
12. Del Bino G, Lassota P, Darzynkiewicz Z: The S phase cytotoxicity of camptopthecin. Exp. Cell Res. 19:27-35, 1991.
13. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH: Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Bioch Bioph Acta 1133:275-285, 1992.
14. Dolbeare F, Beisker W, Pallavicini MG, Vanderlaan M, Gray JW: Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. Cytometry 6:521-530, 1985.
15. Falcieri E, Gobbi P, Sabatelli P, Santi S, Farabegoli F, Rana R, Cataldi A, Maraldi NM, Martelli AM: A combined ultrastructural approach to the study of neclear matrix thermal stabilization. Histochemistry 98:121-129, 1992.
16. Falcieri E, Martelli AM, Bareggi R, Cataldi A, Cocco L: The protein kinase inhibitor induces morphological changes typical of apoptosis in Molt-4 cells without concomitant DNA fragmentation. Biochem Biophys Res Comm 193:19-25, 1993.
17. Fesus L, Davies PJA, Piacentini M: Apoptosis: Molecular mechanisms in programmed cell death. Eur J Cell Bio 56:170-177, 1991.
18. Gorczyca W, Melamed MR, Darzynkiewicz Z: Apoptosis of S-phase HL-60 cells induced by DNA topoisomerase inhibitors: Detection of DNA strand breaks by flow cytometry using the in situ nick translation assay. Toxicol Lett. 67(1-3):249-258, 1993.
19. Lyons AB, Samuel K, Sanderson A, Maddy AH: Simultaneous analysis of immunophenotype and apoptosis of murine thymocytes by single laser flow cytometry. Cytometry 13:809-821, 1992.
20. Mower DA, Peckham DW, Illera VA, Fishbaugh JK, Stunz LL, Ashman RF: Decreased membrane phospholipid packing and decreased cell size precede DNA cleavage in mature mouse B-cell apoptosis. J Immuno 152:4832-4842, 1994.
21. Nicoletti I, Migliorati G, Pagliacci MC, Grignani F, Riccardi C: A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Meth 139:271-279, 1991.
22. Oberhammer F, Wilson JW, Dive C, Morris ID, Hickman JA, Wakeling AE, Walker PR, Sikorska M: Apoptotic death in epithelial cells: Cleavage of DNA to 300 and/or 50kb fragments prior to or in the absence of internucleosomal fragmentation. EMBO J 12:3679-3684, 1993.
23. Ormerod MG, Collins MKL, Rodriguez-Tarduchy G, Robertson D: Apoptosis in interleukin-3 dependent haematopoietic cells: Quantification by two flow cytometric methods. J Immunol Meth 153:57-65, 1992.
24. Ormerod MG, Sun XM, Snowden RT, Davies R, Fearnhead H, Cohen GM: Increased membrane permeability of apoptotic thymocytes: A flow cytometric study. Cytometry 14:595-602, 1993.
25. Schmid I, Uittenbogaart CH, Giorgi JV: Sensitive method for measuring apoptosis and cell surface phenotype in human thymocytes by flow cytometry. Cytometry 15:12-20, 1994.
26. Schmid I, Uittenbogaart CH, Keld B, Giorgi JV: A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J Immunol Meth 170:145-157, 1994.
27. Schulze-Osthoff K, Walczak H, Droge W, Krammer PH: Cell nucleus and DNA fragmentation are not required for apoptosis. J Cell Bio 127:15-20, 1994.
28. Sen S, D'Incalci M: Apoptosis: Biochemical events and relevance to cancer chemotherapy. FEBS 307(1):122-127, 1992.
29. + thymocytes by flow cytometry. J Immunol Meth 137: 79-87, 1991.
30. Telford WG, King LE, Fraker PJ: Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 13:137-143, 1992.
31. Vitale M, Zamai L, Mazzotti G, Cataldi A, Falcieri E: Differential kinetic of propidium iodide uptake in apoptotic and necrotic thymocytes. Histochemistry 100:223-229, 1993.
32. Williams GT, Smith CA: Molecular regulation of apoptosis: genetic controls on cell death. Cell 74:777-779, 1993.
33. Wyllie AH, Kerr JFR, Currie AR: Cell death: the significance of apoptosis. In: International Review of Cytology, Vol. 68, Bourne GH, Danielli FJ, Jeon KW (eds). Academic Press, New York, 1980, pp 251-306.
34. Zamai L, Falcieri E, Zauli G, Cataldi A, Vitale M: Optimal detection of apoptosis by flow cytometry depends on cell morphology. Cytometry 14:891-897, 1993.