Optimized PCR amplification of influenza A virus RNA using Tth DNA polymerase, incorporating uracil N glycosylase (UNG) in a single tube reaction

Journal of Clinical Laboratory Analysis

Volumn 11, Issue 6, 1997, Pages 323-327

  Poddar, Saibal K ^a   Sawyer, Mark H ^a   Connor, James D ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

DNA polymerase; RT PCR; Thermus thermophilus (Tth); Uracil N glycosylase

Indexed keywords

DEOXYURIDINE TRIPHOSPHATE PYROPHOSPHATASE; DNA FRAGMENT; DNA GLYCOSYLTRANSFERASE; DNA POLYMERASE; THYMIDINE TRIPHOSPHATE; URACIL; VIRUS RNA;

ARTICLE; AUTORADIOGRAPHY; GENE AMPLIFICATION; INFLUENZA VIRUS A; NONHUMAN; POLYMERASE CHAIN REACTION; THERMUS THERMOPHILUS; VIRUS DETECTION;

DEOXYURACIL NUCLEOTIDES; DNA GLYCOSYLASES; DNA-DIRECTED DNA POLYMERASE; HUMANS; INFLUENZA A VIRUS; N-GLYCOSYL HYDROLASES; NASOPHARYNX; POLYMERASE CHAIN REACTION; RNA, VIRAL; THERMUS THERMOPHILUS; THYMINE NUCLEOTIDES; URACIL-DNA GLYCOSIDASE;

INFLUENZA A VIRUS; INFLUENZA VIRUS; THERMUS THERMOPHILUS;

EID: 0030782181     PISSN: 08878013     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2825(1997)11:6<323::AID-JCLA2>3.0.CO;2-6     Document Type: Article

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