Binding of mitochondrial precursor proteins to the cytoplasmic domains of the import receptors Tom70 and Tom20 is determined by cytoplasmic chaperones

EMBO Journal

Volumn 16, Issue 14, 1997, Pages 4267-4275

  Komiya, Tohru ^a   Rospert, Sabine ^b   Schatz, Gottfried ^b   Mihara, Katsuyoshi ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b UNIVERSITY OF BASEL   (Switzerland)

Author keywords

Import receptors; Mitochondrial presequences; Mitochondrial protein import; Precursor proteins; Protein targeting

Indexed keywords

ADENINE NUCLEOTIDE TRANSLOCASE; ADENOSINE TRIPHOSPHATE; ADRENODOXIN; CHAPERONE; CYTOPLASM PROTEIN; HEAT SHOCK PROTEIN 70; MEMBRANE RECEPTOR; PORIN; PROTEIN PRECURSOR; SODIUM CHLORIDE;

ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; MITOCHONDRIAL MEMBRANE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RAT; YEAST;

ADENOSINE TRIPHOSPHATE; ADRENODOXIN; AMINO ACID SEQUENCE; ANIMALS; CATTLE; CYTOCHROME P-450 ENZYME SYSTEM; FUNGAL PROTEINS; HSP70 HEAT-SHOCK PROTEINS; MEMBRANE PROTEINS; MEMBRANE TRANSPORT PROTEINS; MITOCHONDRIA; MITOCHONDRIAL ADP, ATP TRANSLOCASES; MOLECULAR CHAPERONES; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PORINS; PROTEIN BINDING; PROTEIN PRECURSORS; RATS; RECEPTORS, CELL SURFACE; SODIUM CHLORIDE; VOLTAGE-DEPENDENT ANION CHANNELS;

ANIMALIA; BOVINAE;

EID: 0030752305     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.14.4267     Document Type: Article

References (26)

1. Allison, D.S. and Schatz, G. (1986) Artificial mitochondrial presequences. Proc. Natl Acad. Sci. USA, 83, 9011-9015.
2. Deshaies, R.J., Koch, B.D., Werner-Washburne, M., Craig, E.A. and Schekman, R. (1988) A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides. Nature, 332, 800-805.
3. Endo, T., Mitsui, S., Nakai, M. and Roise, D. (1996) Binding of mitochondrial presequences to yeast cytosolic heat shock protein 70 depends on the amphiphilicity of the presequence. J. Biol. Chem., 271, 4161-4167.
4. Hachiya, N., Alam, R., Sakasegawa, Y., Sakaguchi, M., Mihara, K. and Omura, T. (1993) A mitochondrial import factor purified from rat liver cytosol is an ATP-dependent conformational modulator for precursor proteins. EMBO J., 12, 1579-1586.
5. Hachiya, N., Komiya, T., Alam, R., Iwahashi, J., Sakaguchi, M., Omura, T. and Mihara, K. (1994) MSF, a novel cytoplasmic chaperone which functions in precursor targeting to mitochondria. EMBO J., 13, 5146-5154.
6. Hachiya, N., Mihara, K., Suda, K., Horst, M., Schatz, G. and Lithgow, T. (1995) Reconstitution of the initial steps of mitochondrial protein import. Nature, 376, 705-709.
7. Hartl, F.-U. (1996) Molecular chaperones in cellular protein folding. Nature, 381, 571-580.
8. Haucke, V., Lithgow, T., Rospert, S., Hahne, K. and Schatz, G. (1995) The yeast mitochondrial protein import receptor Mas20p binds precursor proteins through electrostatic interaction with the positively charged presequence. J. Biol. Chem., 270, 5565-5570.
9. Haucke, V., Horst, M., Schatz, G. and Lithgow, T. (1996) The Mas20p and Mas70p subunits of the protein import receptor of yeast mitochondria interact via the tetratricopeptide repeat motif in Mas20p: evidence for a single hetero-oligomeric receptor. EMBO J., 15, 1231-1237.
10. Hines, V., Brandt, A., Griffiths, G., Horstmann, H., Brutsh, H. and Schatz, G. (1990) Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70. EMBO J., 9, 3191-3200.
11. Iwahashi, J., Furuya, S., Mihara, K. and Omura, T. (1992) Characterization of adrenodoxin precursor expressed in Escherichia coli. J. Biochem., 111, 451-455.
12. Kiebler, M., Keil, P., Schneider, H., van der Klei, I.J., Pfanner, N. and Neupert, W. (1993) Mitochondrial protein import: specific recognition and membrane translocation of preproteins. J. Membr. Biol., 135, 191-207.
13. Komiya, T. and Mihara, K (1996) Protein import into mammalian mitochondria: characterization of the intermediates along the import pathway of the precursor into the matrix. J. Biol. Chem., 271, 22105-22110.
14. Komiya, T., Hachiya, N., Sakaguchi, M., Omura, T. and Mihara, K. (1994) Recognition of mitochondrial targeting signals by a cytosolic import stimulation factor, MSF. J. Biol. Chem., 269, 30893-30897.
15. Komiya, T., Sakaguchi, M. and Mihara, K. (1996) Cytoplasmic chaperones determine the targeting pathway of precursor proteins to mitochondria. EMBO J., 15, 399-407.
16. Lill, R., Nargang, F.E. and Neupert, W. (1996) Biogenesis of mitochondrial proteins. Curr. Opin. Cell Biol., 8, 505-512.
17. Lithgow, T., Glick, B.S. and Schatz, G. (1995) The protein import receptor of mitochondria. Trends Biochem. Sci., 20, 98-101.
18. Mayer, A., Nargang, F.E., Neupert, W. and Lill, R. (1995) MOM22 is a receptor for mitochondrial targeting sequences and cooperates with MOM19. EMBO J., 14, 4204-4211.
19. Mihara, K. and Omura, T. (1996) Cytoplasmic chaperones in precursor targeting to mitochondria: the role of MSF and hsp70. Trends Cell Biol., 6, 104-108.
20. Mihara, K., Blobel, G. and Sato, R. (1982) In vitro synthesis and integration into mitochondria of porin, a major protein of the outer mitochondrial membrane of Saccharomyces cerevisiae. Proc. Natl Acad. Sci. USA, 79, 7102-7106.
21. Moczko, M., Ehmann, B., Gartner, F., Honlinger, A., Schafer, E. and Pfanner, N. (1994) Deletion of the receptor MOM19 strongly impairs import of cleavable preproteins into Saccharomyces cerevisiae mitochondria. J. Biol. Chem., 269, 9045-9051.
22. Schatz, G. and Dobberstein, B. (1996) Common principles of protein translocation across membranes. Science, 271, 1519-1526.
23. Schlossmann, J., Dietmeier, K., Pfanner, N. and Neupert, W. (1994) Specific recognition of mitochondrial preproteins by the cytosolic domain of the import receptor MOM72. J. Biol. Chem., 269, 11893-11901.
24. Sikorski, R.S., Boguski, M.S., Goebl, M. and Hieter, P. (1990) A repeating amino acid motif in CDC23 defines a family of proteins and a new relationship among genes required for mitosis and RNA synthesis. Cell, 60, 307-317.
25. Söllner, T., Griffiths, G., Pfaller, R., Pfanner, N. and Neupert, W. (1989) MOM19, an import receptor for mitochondrial precursor proteins. Cell, 59, 1061-1070.
26. Steger, H.F. Sollner, T., Kiebler, M., Dietmeier, K.A., Pfaller, R., Trulzsch, K.S., Tropschug, M., Neupert, W. and Pfanner, N. (1990) Import of ADP/ATP carrier into mitochondria: two receptors act in parallel. J. Cell Biol., 111, 2353-2363.