Regulation and physiological role of the DAS1 gene, encoding dihydroxyacetone synthase, in the methylotrophic yeast Candida boidinii

Journal of Bacteriology

Volumn 180, Issue 22, 1998, Pages 5885-5890

  Sakai, Yasuyoshi ^a   Nakagawa, Tomoyuki ^a   Shimase, Masayuki ^a   Kato, Nobuo ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL OXIDASE; CARBON; CHOLINE; DIHYDROXYACETONE; FORMALDEHYDE; FORMALDEHYDE DEHYDROGENASE; FORMATE DEHYDROGENASE; MESSENGER RNA; METHANOL; METHYLAMINE; NITROGEN; SYNTHETASE;

ARTICLE; CANDIDA BOIDINII; CONTROLLED STUDY; CULTURE MEDIUM; DETOXIFICATION; FUNGAL GENETICS; FUNGUS GROWTH; GENE DISRUPTION; GENE EXPRESSION REGULATION; GENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL;

CANDIDA BOIDINII; FUNGI; MYXOGASTRIA;

EID: 0031734177     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.22.5885-5890.1998     Document Type: Article

References (39)

1. Bergmeyer, H. U. 1955. Zur Messung von Katalase Aktivitäten. Biochem. Z. 327:255-258.
2. Bystrykh, L. V., L. R. Aminova, and Y. A. Trotsenko. 1988. Methanol metabolism in mutants of the methylotrophic yeast, Hansenula polymorpha. FEMS Microbiol. Lett. 51:89-94.
3. Bystrykh, L. V., A. P. Sokolov, and Y. A. Trotsenko. 1981. Purification and properties of dihydroxyacetone synthase from the methylotrophic yeast, Candida boidinii. FEBS Lett. 132:324-328.
4. Cryer, D. R., R. Eccleshal, and J. Murmer. 1975. Isolation of yeast DNA. Methods Cell Biol. 12:39-44.
5. Davis, R. W., M. Thomas, J. Cameron, T. P. St. John, S. Scherer, and R. A. Padgett. 1980. Rapid DNA isolations for enzymatic and hybridization analysis. Methods Enzymol. 65:404-411.
6. Feinberg, A. P., and B. Vogelstein. 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:6-13.
7. Fletcher, T. S., I. L. Kwee, T. Nakada, C. Largman, and B. M. Martin. 1992. DNA sequence of the yeast transketolase gene. Biochemistry 31:1892-1896.
8. Garrard, L. J., and J. M. Goodman. 1989. Two genes encode the major membrane-associated protein of methanol-induced peroxisomes from Candida boidinii. J. Biol. Chem. 264:13929-13937.
9. 8a. Goodman, J. M. Unpublished data.
10. Hawkins, C. F., A. Borges, and P. N. Perham. 1989. A common structural motif in thiamin pyrophosphate-binding enzymes. FEBS Lett. 255:77-82.
11. Janowicz, Z., M. Eckart, C. Drewke, R. Roggenkamp, C. P. Hollenberg, J. Maat, A. M. Ledeboer, C. Visser, and C. T. Verrips. 1985. Cloning and characterization of the DAS gene encoding the major methanol assimilatory enzyme from the methylotrophic yeast Hansenula polymorpha. Nucleic Acids Res. 13:3043-3062.
12. Kato, N., T. Higuchi, C. Sakazawa, T. Nishizawa, Y. Tani, and H. Yamada. 1982. Purification and properties of a transketolase responsible for formaldehyde fixation in a methanol-utilizing yeast. Candida boidinii (Kloeckera sp.) no. 2201. Biochim. Biophys. Acta 715:143-150.
13. Kato, N., T. Nishizawa, C. Sakazawa, Y. Tani, and H. Yamada. 1979. Xylulose 5-phosphate-dependent fixation of formaldehyde in a methanol-utilizing yeast. Kloeckera sp. no. 2201. Agric. Biol. Chem. 43:2013-2015.
14. Koning, W. D., K. Bonting, W. Harder, and L. Dijkhuizen. 1990. Classical transketolase functions as the formaldehyde-assimilating enzyme during growth of a dihydroxyacetone synthase-negative mutant of the methylotrophic yeast Hansenula polymorpha on mixtures of xylose and methanol in continuous culture. Yeast 6:117-125.
15. Koning, W. D., M. A. G. Gleeson, W. Harder, and L. Dijkhuizen. 1987. Regulation of methanol metabolism in the yeast Hansenula polymorpha. Isolation and characterization of mutants blocked in methanol assimilatory enzymes. Arch. Microbiol. 147:375-382.
16. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
17. Lindqvist, Y., G. Schneider, U. Ermler, and M. Sundstrom. 1992. Three-dimensional structure of transketolase, a thiamine diphosphate dependent enzyme, at 2.5 Å resolution. EMBO J. 11:2373-2379.
18. Metzger, M. H., and C. P. Hollenberg. 1994. Isolation and characterization of the Pichia stipitis transketolase gene and expression in a xylose utilising Saccharomyces cerevisiae transformant. Appl. Microbiol. Biotechnol 42:319-325.
19. Mori, N., K. Shirakawa, K. Uzura, Y. Kitamoto, and Y. Ichikawa. 1988. Formation of ethylene glycol and trimethylamine from choline by Candida tropicalis. FEMS Microbiol. Lett. 51:41-44.
20. Nash, T. 1953. The colorimetric estimation of formaldehyde by means of the Hantzsch reaction. Biochem. J. 55:416-421.
21. Sakai, Y., M. Akiyama, H. Kondoh, Y. Shibano, and N. Kato. 1996. High-level secretion of fungal glucoamylase using the Candida boidinii gene expression system. Biochim. Biophys. Acta 1308:81-84.
22. Sakai, Y., T. K. Goh, and Y. Tani. 1993. High-frequency transformation of a methylotrophic yeast, Candida boidinii, with autonomously replicating plasmids which are also functional in Saccharomyces cerevisiae. J. Bacteriol. 175:3556-3562.
23. Sakai, Y., T. Kazarimoto, and Y. Tani. 1991. Transformation system for an asporogenous methylotrophic yeast, Candida boidinii: cloning of the orotidine-5′-phosphate decarboxylase gene (URA3), isolation of uracil auxotrophic mutants, and use of the mutants for integrative transformation. J. Bacteriol. 173:7458-7463.
24. Sakai, Y., A. P. Murdanoto, T. Konishi, A. Iwamatsu, and N. Kato. 1997. Regulation of the formate dehydrogenase gene, FDH1, in the methylotrophic yeast Candida boidinii and growth characteristics of an FDH1-disrupted strain on methanol, methylamine, and choline. J. Bacteriol. 179:4480-4485.
25. Sakai, Y., T. Rogi, R. Takeuchi, N. Kato, and Y. Tani. 1995. Expression of Saccharomyces adenylate kinase gene in Candida boidinii under the regulation of its alcohol oxidase promoter. Appl. Microbiol. Biotechnol. 42:860-864.
26. Sakai, Y., T. Rogi, T. Yonehara, N. Kato, and Y. Tani. 1994. High-level ATP production by a genetically-engineered Candida yeast. Bio/Technology 12: 291-293.
27. Sakai, Y., A. Saiganji, H. Yurimoto, K. Takabe, H. Saiki, and N. Kato. 1996. The absence of Pmp47, a putative yeast peroxisomal transporter, causes defect in transport and folding of a specific matrix enzyme. J. Cell Biol. 134:37-51.
28. Sakai, Y., and Y. Tani. 1992. Cloning and sequencing of the alcohol oxidase-encoding gene (AOD1) from the formaldehyde-producing asporogenous methylotrophic yeast, Candida boidinii S2. Gene 114:67-73.
29. Sakai, Y., and Y. Tani. 1992. Directed mutagenesis in an asporogenous methylotrophic yeast: cloning, sequencing, and one-step gene disruption of the 3-isopropylmalate dehydrogenase gene (LEU2) of Candida boidinii to derive doubly auxotrophic marker strains. J. Bacteriol. 174:5988-5993.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
32. Schaaff-Gerstenschlager, I., G. Mannhaupt, I. Vetter, F. K. Zimmermann, and H. Feldmann. 1993. TKL2, a second transketolase gene of Saccharomyces cerevisiae; cloning, sequence and deletion analysis of the gene. Eur. J. Biochem. 217:487-492.
33. Schenk, G., R. Layfield, J. M. Candy, R. G. Duggleby, and P. F. Nixon. 1997. Molecular evolutionary analysis of the thiamine-diphosphate-dependent enzyme, transketolase. J. Mol. Evol. 44:552-572.
34. Schütte, H., J. Flossdorf, H. Sahm, and M.-R. Kula. 1976. Purification and properties of formaldehyde dehydrogenase and formate dehydrogenase from Candida boidinii. Eur. J. Biochem. 62:151-160.
35. 2 are non-essential for energy supply of yeast methylotrophic growth. Arch. Microbiol. 154:566-575.
36. Tani, Y., Y. Sakai, and H. Yamada. 1985. Isolation and characterization of a mutant of a methanol yeast, Candida boidinii S2, with higher formaldehyde productivity. Agric. Biol. Chem. 49:2699-2706.
37. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
38. Tschopp, J. F., P. F. Burst, J. M. Cregg, S. A. Stillman, and T. R. Gingeras. 1987. Expression of a lacZ gene from two methanol-regulated promoters in Pichia pastoris. Nucleic Acids Res. 15:3859-3876.
39. 13C]methanol and hydroxypyruvate using the methanol-assimilating system of methylotrophic yeasts. Appl. Microbiol. Biotechnol. 43:228-234.