Differentiation of Actinobacillus pleuropneumoniae strains by sequence analysis of 16S rDNA and ribosomal intergenic regions, and development of a species specific oligonucleotide for in situ detection

Systematic and Applied Microbiology

Volumn 21, Issue 3, 1998, Pages 408-418

  Fussing, Vivian ^a,b   Paster, Bruce J ^c   Dewhirst, Floyd E ^c   Poulsen, Lars K ^a,d  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b STATENS SERUM INSTITUT   (Denmark)

^c FORSYTH INSTITUTE   (United States)

^d NOVO NORDISK A S   (Denmark)

Author keywords

16S rRNA; Actinobacillus pleuropneumoniae; In situ detection; Ribosomal intergenic region; Ribosomal operons

Indexed keywords

FLUORESCEIN; RIBONUCLEASE III; RIBOSOME DNA; RNA 16S; RNA 23S; TRANSFER RNA;

ACTINOBACILLUS PLEUROPNEUMONIAE; ARTICLE; CELL DIFFERENTIATION; ESCHERICHIA COLI; IN SITU HYBRIDIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ACTINOBACILLUS LIGNIERESII; ACTINOBACILLUS PLEUROPNEUMONIAE; ACTINOBACILLUS SUIS; ESCHERICHIA COLI; POSIBACTERIA; SUIDAE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0031719812     PISSN: 07232020     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0723-2020(98)80050-7     Document Type: Article

References (43)

1. AMANN, R. I., KRUMHOLZ, L., STAHL, D. A.: Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172, 762-770 (1990).
2. AMIKAN, D., KUHN, J.: Characterization of Vibrio fischeri rRNA operons and subcloning of a ribosomal DNA promoter. J. Bacteriol. 169, 2318-2321 (1987).
3. BECK, M., VAN DEN BOSCH, J. F., JONGENELEN, I. M. C. A., LOEFFEN, P. L. W., NIELSEN, R., NICOLET, J., FREY, J.: RTX toxin genotypes and phenotypes in Actinobacillus pleuropneumoniae field strains. J. Clin. Microbiol. 32, 2749-2754 (1994).
4. BORR, J. D., RYAN, D. A., MACINNES, J. I.: Analysis of Actinobacillus pleuropneumoniae and related organisms by DNA-DNA hybridization and restriction endonuclease fingerprinting. Int. J. Syst. Bacteriol. 41, 121-129 (1991).
5. CHRISTENSEN, J. P., OLSEN, J. E., BISGAARD, M.: Ribotypes of Salmonella enterica serovar Gallinarum biovars gallinarum and pullorum. Avian Pathol. 22, 725-738 (1993).
6. DEWHIRST, F. E., PASTER, B. J., OLSEN, I., FRASER, G. J.: Phytogeny of 54 representative strains of species in the family Pasteurellaceae as determined by comparison of 16S rRNA sequences. J. Bacteriol. 174, 2002-2013 (1992).
7. FODOR, L., VARGA, J., MOLNÁR, É., HAJTÓS, I.: Biochemical and serological properties of Actinobacillus pleuropneumoniae biotype 2 strains isolated from swine. Vet. Microbiol. 20, 173-180 (1989).
8. GÜRTLER, V., BARRIE, H. D.: Typing of Staphylococcus aureus strains by PCR-amplification of variable-length 16S-23S rDNA spacer regions: characterization of spacer sequences. Microbiol. 141, 1255-1265 (1995).
9. GÜRTLER, V., STANISICH, V. A.: New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiol. 142, 3-16 (1996).
10. HAMPSON, D. J., BLACKALL, P. J., WOODWARD, J. M., LYMBERY, A. J.: Genetic analysis of Actinobacillus pleuropneumoniae, and comparison with Haemophilus spp. taxon "Minor Group" and taxon C. Zbl. bakt. 279, 83-91 (1993).
11. INZANA, T. J.: Virulence properties of Actinobacillus pleuropneumoniae. Microb. Pathog. 11, 305-316 (1991).
12. JENSEN, M. A., WEBSTER, J. A., STRAUS, N.: Rapid identification of bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphisms. Appl. Env. Microbiol. 59, 945-952 (1993).
13. KILIAN, M., NICOLET, J., BIBERSTEIN, E. L.: Biochemical and serological characterization of Haemophilus pleuropneumoniae (Matthews and Pattison 1961) Shope 1964 and proposal of a neotype strain. Int. J. Syst. Bacteriol. 28, 20-26 (1978).
14. KING, T. C., SIRDESKMUKH, R., SCHLESSINGER, D.: Nucleolytic processing of ribonucleic acid transcripts in procaryotes. Microbiol. Rev. 50, 428-451 (1986).
15. KOSTMAN, J. R., EDLIND, T. D., LIPUMA, J. J., STULL, T. L.: Molecular epidemiology of Pseudomonas cepacia determined by polymerase chain reaction ribotyping. J. Clin. Microbiol. 30, 2084-2087 (1992).
16. KROGFELT, K. A., POULSEN, L. K., MOLIN, S.: Identification of coccoid Escherichia coli BJ4 cells in the large intestine of streptomycin-treated mice. Infect. Immun. 61, 5029-5034 (1993).
17. LANE, D. 16S/23S Sequencing, pp. 115-147. In: Nucleic acid techniques in bacterial systematics (E. STACKEBRANDT, M. GOODFELLOW, eds.) New York, John Wiley and Sons 1991.
18. LEYS, E. J., GRIFFEN, A. L., STRONG, S. J., FUERST, P. A.: Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR. J. Clin. Microbiol. 32, 1288-1294 (1994).
19. MACINNES, J. I., BORR, J. D., MASSOUDI, M., ROSENDAL, S.: Analysis of southern Ontario Actinobacillus (Haemophilus) pleuropneumoniae isolates by restriction endonuclease fingerprinting. Can. J. Vet. Res. 54, 244-250 (1990).
20. MAIDAK, B. L., OLSEN, G. J., LARSEN, N., OVERBEEK, R., MCCAUGHEY, M. J., WOESE, C. R.: The RDP (Ribosomal Database Project). Nucleic Acids Res. 25, 109-111 (1997).
21. MANIATIS, T., FRITSCH, E. F., SAMBROOK, J.: Molecular cloning, a laboratory manual, 1-545 pp. New York, Cold Spring Harbor Laboratory 1987.
22. MANNHEIM, W.: Pasteurellaceae, pp. 550-569. In: Bergey's manual of systematic bacteriology (N. R. KRIEG, ed.) 8th ed., Baltimore, Williams and Wilkins 1984.
23. MATAR, G. M., SWAMINATHAN, B., HUNTER, S. B., SLATER, L. N., WELCH, D. F.: Polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from Rochalimaea species for subtyping. J. Clin. Microbiol. 31, 1730-1734 (1993).
24. MORGAN, E. A., IKEMURA, T., NOMURA, M.: Identification of spacer tRNA genes in individual ribosomal RNA transcription units of Escherichia coli. Proc. Natl. Acad. Sci. USA 74, 2710-2714 (1977).
25. MUSSER, J. M., RAPP, V. J., SELANDER, R. K.: Clonal diversity in Haemophilus pleuropneumoniae. Infect. Immun. 55, 1207-1215 (1987).
26. MUTTERS, R., PIECHULLA, K., MANNHEIM, W.: Phenotypic differencies of Pasteurella senso stricto in the Actinobacillus group. Eur. J. Clin. Microbiol. 3, 225-229 (1984).
27. MØLLER, K., NIELSEN, R., ANDERSEN, L. V., KILIAN, M.: Clonal analysis of the Actinobacillus pleuropneumoniae population in a geographically restricted area by multilocus enzyme electrophoresis. J. Clin. Microbiol. 30, 623-627 (1992).
28. NICOLET, J.: Sur l'hemophilose du porc III. Differenciation serologic de Haemophilus parahaemolyticus. Zbl. bakt. 216, 487-495 (1971).
29. NICOLET, J.: Actinobacillus pleuropneumoniae, pp. 401-408. In: Diseases of swine (A. D. Leman, B. E. Straw, W. L. Mengeling, S. D'Allaire, D. J. Taylor, eds.) 7th ed., London, Wolfe Publishing Ltd. 1992.
30. NIELSEN, R.: Serological Characterization of Actinobacillus pleuropneumoniae strains and proposal of a new serotype: serotype 12. Acta Vet. Scand. 27, 453-455 (1986).
31. NIELSEN, R.: Seroepidemiology of Actinobacillus pleuropneumoniae. Can. Vet. J. 29, 580-582 (1988).
32. NIELSEN, R., ANDRESEN, L. O., PLAMBECK, T.: Serological characterization of Actinobacillus pleuropneumoniae biotype 1 strains antigenically related to both serotypes 2 and 7. Acta Vet. Scand. 37, 327-336 (1996).
33. NIELSEN, R., ANDRESEN, L. O., PLAMBECK, T., NIELSEN, J. P., KRARUP, L. T., JORSAL, S. E.: Serological characterization of Actinobacillus pleuropneumoniae biotype 2 strains isolated from pigs in 2 Danish herds. Vet. Microbiol. 54, 35-46 (1997).
34. PASTER, B. J., DEWHIRST, F. E., COOKE, S. M., FUSSING, V., POULSEN, L. K. AND BREZNAK, J. A.: Phylogeny of not-yet-cultured spirochetes from termite guts. Appl. Env. Microbiol. 62, 347-352 (1996).
35. PHILLIPS, J. E.: The incidence of agglutinating antibodies to Actinobacillus lignieresi in the sera of normal and infected cattle. J. Pathol. Bacteriol. 90, 557-566 (1965).
36. PHILLIPS, J. E.: Antigenic structure and serological typing of Actinobacillus lignieresi. J. Pathol. Bacteriol. 93, 463-475 (1967).
37. POHL, S., BERTSCHINGER, H. U., FREDERIKSEN, W., MANNHEIM, W.: Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae comb. nov.) on the basis of phenotypic and deoxyribonucleic acid relatedness. Int. J. Syst. Bacteriol. 33, 510-514 (1983).
38. POULSEN, L. K., FUSHENG, L., KRISTENSEN, C. S., HOBOLTH, P., MOLIN, S., KROGFELT, K. A.: Spatial distribution of Escherichia coli in the mouse large intestine inferred from rRNA in situ hybridization. Infect. Immun. 62, 5191-5194 (1994).
39. STACKEBRANDT, E., GOEBEL, B. M.: Taxonomic note: A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol. 44, 846-849 (1994).
40. STAHL, D. A., AMANN, R. I.: Development and application of nucleic acid probes, pp. 205-248. In: Nucleic acid techniques in bacterial systematics. (E. STACKEBRANDT, M. GOODFELLOW, eds.) New York, John Wiley and Sons 1991.
41. TASCHKE, C., KLINKERT, M., WOLTERS, J., HERRMANN, R.: Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: The 5S rRNA gene is separated from the 16S and 23S rRNA genes. Mol. Gen. Genet. 205, 428-433 (1986).
42. WOESE, C. R.: Bacterial Evolution. Microbiol. Rev. 51, 221-271 (1987).
43. YOUNG, R. A., MACKLIS, R., STEITZ, J. A.: Sequence of the 16S-23S spacer region in two ribosomal RNA operons of Escherichia coli. J. Biol. Chem. 254, 3264-3271 (1979).