Minimum number of adult human retinal pigment epithelial cells required to establish a confluent monolayer in vitro

Current Eye Research

Volumn 17, Issue 10, 1998, Pages 962-969

  Kim, Kwang Soo ^a   Tezel, Tongalp H ^a   Del Priore, Lucian V ^a,b  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b WASHINGTON UNIVERSITY   (United States)

Author keywords

Cell culture; Cell growth; Human; Retinal pigment epithelium (RPE)

Indexed keywords

ARTICLE; CELL DENSITY; CELL STRUCTURE; CELL VIABILITY; CORNEA ENDOTHELIUM; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; MONOLAYER CULTURE; PIGMENT EPITHELIUM; PRIORITY JOURNAL;

AGED; AGED, 80 AND OVER; ANIMALS; CATTLE; CELL COUNT; CELL DIVISION; CELL SURVIVAL; CELLS, CULTURED; EXTRACELLULAR MATRIX; HUMANS; KERATINS; MIDDLE AGED; PIGMENT EPITHELIUM OF EYE; TIME FACTORS;

EID: 0031713950     PISSN: 02713683     EISSN: None     Source Type: Journal    
DOI: 10.1076/ceyr.17.10.962.5243     Document Type: Article

References (37)

1. Del Priore LV, Kaplan HJ, Silvermann MS, Valentino T, Mason G, Hornbeck, R. Experimental and surgical aspects of retinal pigment epithelial cell transplantation. Eur J Implant Ref Surg. 1993;5:128-132.
2. Silbert JE, Gao EK, Yu X-H, Kaplan HJ. Adult murine retinal pigment epithelium is immunogenic. Invest Ophthalmol Vis Sci. 1994;35(suppl 4):S1525.
3. Jiang LQ, Jorquera M, Streilein JW. Immunologic consequences of intraocular implantation of retinal pigment epithelial allografts. Exp Eye Res. 1994;58:719-728.
4. Algvere PV, Berglin L, Gouras P, Sheng Y, Kopp ED. Transplantation of RPE in age-related macular degeneration: observations in disciform lesions and dry RPE atrophy. Graefe's Arch Clin Exp Ophthalmol. 1997;235:149-158.
5. Peyman GA, Blinder KJ, Paris CL, Alturki W, Nelson NC Jr, Desai U. A technique for retinal pigment epithelium transplantation for age-related macular degeneration secondary to extensive subfoveal scarring. Ophthalmic Surg. 1991;22:102-108.
6. Hornbeck R, Tezel TH, Del Priore LV Use of fibrin glue for autologous RPE transplantation. Invest Ophthalmol Vis Sci. 1996;32(suppl3):S1858.
7. Zarbin MA, Ishida M, Yamani A, et al. Culture of human RPE from scleral flap biopsies. Exp Eye Res. 1996;63 (suppl 1):S193.
8. Song M, Lui GM. Propagation of fetal human RPE cells: preservation of original culture morphology after serial passage. J Cell Physiol. 1990;143:196-203.
9. Gospodarowicz DC III. The extracellular matrix and the control of proliferation of corneal endothelial and lens epithelial cells. Exp Eye Res. 1980;31:181-199.
10. Pfeffer B. Improved methodology for cell culture of human and monkey retinal pigment epithelium. Prog Retinal Res. 1991;10:251-291.
11. Tezel TH, Del Priore LV, Kaplan HJ. Harvest and storage of adult human retinal pigment epithelial sheets. Curr Eye Res. 1997;16:802-809.
12. Hunt RC, Davis AA. Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990;145:187-199.
13. McKechnie NM, Boulton M, Robey HL, Savage FJ, Grierson I. The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo. J Cell Sci. 1988;91:303-312.
14. Haugland RP, Larison KD. Fluorescent dyes for assessing vital cell functions. In: Handbook of Fluorescent Probes and Research Chemicals. 5th ed. Eugene, OR: Molecular Probes; 1994:172-180.
15. Dawson-Saunders B, Trapp RG. Association and prediction. In: Basic and Clinical Biostatistics. 2nd ed. Norwalk, CT: Appleton and Lange; 1994:162-187.
16. Daniel WW. Analysis of variance. In: Biostatistics. A Foundation for Analysis in the Health Sciences. 6th ed. New York, NY: John Wiley & Sons; 1995:273-352.
17. Dafgård Kopp EME, Winter-Vernersson AM, Algvere PV. Expression of HLA class I and II antigens in human fetal RPE cells. Invest Ophthalmol Vis Sci. 1997;38(suppl 4): S947.
18. Wongpichedchai S, Weiter JJ, Weber P, Dorey CK. Comparison of external and internal approaches for transplantation of autologous retinal pigment epithelium. Invest Ophthalmol Vis Sci. 1992;33:3341-3352.
19. Tezel TH, Del Priore LV, Kaplan HJ. Fate of adult human retinal pigment epithelium (RPE) plated onto different layers of human Bruch's membrane (BM) in vitro. Invest Ophthalmol Vis Sci. 1998;39(suppl):S716 Abstract 3361.
20. Del Priore LV, Tezel TH. Reattachment rate of human retinal pigment epithelium to layers of human Bruch's membrane. Arch Ophthalmol. 1998;116:335-341.
21. Jones Z, Tezel TH, Del Priore LV Morphology of retinal pigment epithelium (RPE) after reattachment to different layers of human Bruch's membrane. Invest Ophthalmol Vis Sci. 1998;39(suppl):S98 Abstract 460.
22. Tezel TH, Del Priore LV Density-dependent growth characteristics of porcine retina pigment epithelial (RPE) cells. Graefe's Arch Clin Exp Ophthalmol. 1996;234(suppl 1): S89-95.
23. McKay BS, Burke JM. Separation of phenotypically distinct subpopulations of cultured human retinal pigment epithelial cells. Exp Cell Res. 1994;213:85-92.
24. Poulding RH. Culture of fetal cells from amniotic fluid. In: Wasley GD. Animal tissue culture: Advances in technique. Baltimore, MD: Williams & Wilkins; 1973:180.
25. Gnepp DR, Chandler W. Tissue culture of human and canine thoracic duct endothelium. In Vitro Cell Dev Biol. 1985;21:200-206.
26. Chen WF, Ewing T, Scollay R, Shortman K. Growth of single T cells and single thymocytes in a high cloning efficiency filler-cell free microculture system. Thymus. 1988; 12:51-68.
27. Jonas JB, Muller-Bergh JA, Schlotzer-Schrehardt UM, Naumann GO. Histomorphometry of the human optic nerve. Invest Ophthalmol Vis Sci. 1990;31:736-744.
28. Panda-Jonas S, Jonas JB, Jakobczykzmija M. Retinal pigment epithelial cell count, distribution, and correlations in normal human eyes. Am J Ophthalmol. 1996;121:181-189.
29. Crawford B. Cloned pigmented retinal epithelium: the role of microfilaments in the differentiation of cell shape. J Cell Biol. 1979;81:301-315.
30. Matsumoto B, Guerin CJ, Anderson DH. Cytoskeletal redifferentiation of feline, monkey, and human RPE cells in culture. Invest Ophthalmol Vis Sci. 1990;31:879-889.
31. Miceli MV, Newsome DA. Effects of extracellular matrix and Bruch's membrane on retinal outer segment phagocytosis by cultured human retinal pigment epithelium. Curr Eye Res. 1996;15:17-26.
32. McLaren MJ. Kinetics of rod outer segment phagocytosis by cultured retinal pigment epithelial cells: relationship to cell morphology. Invest Ophthalmol Vis Sci. 1996;37:1213-1224.
33. Grisanti S, Guidry C. Transdifferentiation of retinal pigment epithelial cells from epithelial to mesenchymal phenotype. Invest Ophthalmol Vis Sci. 1995;36:391-405.
34. Campochiaro PA, Hackett SF. Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: implication of laminin and basic fibroblast growth factor as active components. Exp Eye Res. 1993;57:539-547.
35. Burke JM, Soref C. Topographical variation in growth in cultured bovine retinal pigment epithelium. Invest Ophthalmol Vis Sci. 1988;29:1784-1788.
36. Opas M, Dziak E. Effects of substrata and method of tissue dissociation on adhesion, cytoskeleton, and growth of chick retinal pigmented epithelium in vitro. In Vitro Cell Dev Biol. 1988;24:885-892.
37. Flood MT, Gouras P, Kjeldbye H. Growth characteristics and ultrastructure of human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci. 1980;19:1309-1320.