Kinetics of rod outer segment phagocytosis by cultured retinal pigment epithelial cells: Relationship to cell morphology

Investigative Ophthalmology and Visual Science

Volumn 37, Issue 7, 1996, Pages 1213-1224

  McLaren, Margaret J ^a  

^a UNIVERSITY OF MIAMI MILLER SCHOOL OF MEDICINE   (United States)

Author keywords

cell culture; phagocytosis; retinal degeneration; retinal pigment epithelium (RPE); rod outer segments (ROS)

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL SUBPOPULATION; CONTROLLED STUDY; EPITHELIUM CELL; NONHUMAN; PHAGOCYTOSIS; PIGMENT EPITHELIUM; PRIORITY JOURNAL; RAT; RETINA ROD OUTER SEGMENT;

ANIMALS; CELL SIZE; CELLS, CULTURED; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; KINETICS; MICROSCOPY, FLUORESCENCE; PHAGOCYTOSIS; PHENOTYPE; PIGMENT EPITHELIUM OF EYE; RATS; RATS, MUTANT STRAINS; RETINAL DEGENERATION; ROD OUTER SEGMENTS;

EID: 0029948961     PISSN: 01460404     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Hall MO, Abrams T, Mittag TW. ROS ingestion by RPE cells is turned off by increased protein kinase activity and by increased calcium. Exp Eye Res. 1991;52:591-598.
2. Boyle DL, Tien L, Cooper NGF, Shepherd V, McLaughlin BJ. A mannose receptor is involved in retinal phagocytosis. Invest Ophthalmol Vis Sci. 1991;32:1464-1470.
3. Edwards RB. Stimulation of rod outer segment phagocytosis by serum occurs only at the RPE apical surface. Exp Eye Res. 1991;53:229-232.
4. Heth CA, Schmidt SY. Phagocytic challenge induces changes in phosphorylation of retinal pigment epithelium proteins. Curr Eye Res. 1991;10:1049-1057.
5. Gregory CY, Hall MO. The phagocytosis of ROS by RPE cells is inhibited by an antiserum to rat RPE cell plasma membranes. Exp Eye Res. 1992;54:843-851.
6. Heth CA, Schmidt SY. Protein phosphorylation in retinal pigment epithelium of Long-Evans and Royal College of Surgeons rats. Invest Ophthalmol Vis Sci. 1992;33:2839-2847.
7. Reid DM, Laird DW, Molday RS. Characterization and application of an in vitro detection system for studying the binding and phagocytosis of rod outer segments by retinal pigment epithelial cells. Exp Eye Res. 1992 54:775-783.
8. McLaren MJ, Inana G, Li C-Y. Double fluorescent vital assay of phagocytosis by cultured retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1993;34:317-326.
9. Hall MO, Abrams TA, Mittag TW. The phagocytosis of rod outer segments is inhibited by drugs linked to cyclic adenosine monophosphate production. Invest Ophthalmol Vis Sci. 1993;34:2392-2401.
10. Kennedy CJ, Rakoczy PE, Robertson TA, Papadmitriou JM, Constable IJ. Kinetic studies on phagocytosis and lysosomal digestion of rod outer segments by human retinal pigment epithelial cells in vitro. Exp Cell Res. 1994;210:209-214.
11. McLaughlin BJ, Cooper NGF, Shepherd VL. How good is the evidence to suggest that phagocytosis of ROS by RPE is receptor mediated? In: Osborne NN, Chader GJ eds. Progress in Retinal and Eye Research. Oxford: Pergamon Press; 1994;13:147-164.
12. Edwards RB. Culture of rat retinal pigment epithelium. In Vitro. 1977;13:301-304.
13. Edwards RB. The isolation and culturing of retinal pigment epithelium of the rat. Vision Res. 1981;21: 147-150.
14. Mayerson PL, Hall MO, Clark V, Abrams T. An improved method for isolation and culture of rat retinal pigment epithelial cells. Invest Ophthalmol Vis Sci. 1985;26:1599-1609.
15. Chang C-W, Roque RS, Defoe DM, Caldwell RB. An improved method for isolation and culture of pigment epithelial cells from rat retina. Curr Eye Res. 1991; 10:1081-1086.
16. McLaren MJ, Holderby M, Brown ME, Inana G. Kinetics of ROS binding and ingestion by cultured RCS rat RPE cells: Modulation by conditioned media and bFGF. ARVO Abstracts. Invest Ophthalmol Vis Sci. 1992;33:1027.
17. McLaren MJ, Inana G. ROS phagocytosis by cultured normal rat RPE and bFGF-treated dystrophic RPE is transcriptionally controlled. ARVO Abstracts. Invest Ophthalmol Vis Sci. 1995;36:5815.
18. McLaren MJ, Sasabe T, Li G-Y, Brown ME, Inana G. Spontaneously arising immortal cell line of rat retinal pigmented epithelial cells. Exp Cell Res. 1993;204:311-320.
19. Chaitin MH, Hall MO. Defective ingestion of rod outer segments by cultured dystrophic rat pigment epithelial cells. Invest Ophthalmol Vis Sci. 1983;24:812-820.
20. Turksen K, Opas M, Aubin JE, Kalnins VI. Microtubules, microfilaments and adhesion patterns in differentiating chick retinal pigment epithelial (RPE) cells in vitro. Exp Cell Res. 1983;147:379-391.
21. Turksen K, Opas M, Kalnins VI. Cytoskeleton, adhesion, and extracellular matrix of fetal human retinal pigment epithelial cells in culture. Ophthalmic Res. 1989;21:56-66.
22. Hall MO, Abrams T. Kinetic studies of rod outer segment binding and ingestion by cultured rat RPE cells. Exp Eye Res. 1987;45:907-922.
23. Hall MO, Abrams TA. RPE cells from normal rats do not secrete a factor which enhances the phagocytosis of ROS by dystrophic rat RPE cells. Exp Eye Res. 1991;52:461-464.
24. LaVail MM. Rod outer segment disk shedding in rat retina: Relationship to cyclic lighting. Science. 1976; 192:799-801.
25. Turksen K, Kalnins V. The cytoskeleton of chick retinal pigment epithelial cells in situ. Cell Tissue Res. 1987;248:95-101.
26. Opas M. Substratum mechanics and cell differentiation. Int Rev Cytol. 1994;150:119-137.
27. Hynes RO. Integrins: Versatility, modulation and signaling in cell adhesion. Cell. 1992;69:11-25.
28. Damsky CH, Werb Z. Signal transduction by integrin receptors for extracellular matrix: Cooperative processing of extracellular information. Curr Opin Cell Biol. 1992;4:772-781.
29. Philp NJ, Nachmias VT. Polarized distribution of integrin and fibronectin in retinal pigment epithelium. Invest Ophthalmol Vis Sci. 1987;28:1275-1280.
30. Rizzolo LJ. The polarity of the retinal pigment epithelium is developmentally regulated. Exp Eye Res. 1991;53:549-553.
31. Rizzolo LJ. Basement membrane stimulates the polarized distribution of integrins but not the Na,K-APTase in the retinal pigment epithelium. Cell Reg.. 1991; 2:939-949.
32. Rizzolo LJ, Zhou S, Li Z-Q. The neural retina maintains integrins in the apical membrane of the RPE early in development. Invest Ophthalmol Vis Sci. 1994;35:2567-2576.
33. Ryeom SW, Silverstein RL, Sparrow JR. The absence of CD36, a molecule mediating phagocytosis of photo-receptor outer segments (ROS) correlates with a lack of ROS uptake by retinal pigment epithelial (RPE) cells. ARVO Abstracts. Invest Ophthalmol Vis Sci. 1995;36:S815.
34. Ershov AV, Lukiv WJ, Bazan NG. Phagocytosis of rod outer segments (ROS) triggers die expression of zinc finger-containing transcription factor ZIF 268 in cultured rat pigment epithelial (RPE) cells. ARVO Abstracts. Invest Ophthalmol Vis Sci. 1995;36:S815.