Dietary amino acids are the preferential source of hepatic protein synthesis in piglets

Journal of Nutrition

Volumn 128, Issue 9, 1998, Pages 1517-1524

  Stoll, Barbara ^a   Burrin, Douglas G ^a   Henry, Joseph ^a   Yu, Hung ^a   Jahoor, Farook ^a   Reeds, Peter J ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Hepatic amino acid metabolism; Hepatic protein synthesis; Metabolic compartmentation; Pigs; Stable isotopes

Indexed keywords

AMINO ACID; APOLIPOPROTEIN B100; CARBON 13; LEUCINE; LYSINE; PHENYLALANINE; THREONINE;

AMINO ACID METABOLISM; ANIMAL EXPERIMENT; ARTICLE; DIETARY INTAKE; ISOTOPE LABELING; LIVER METABOLISM; NONHUMAN; PROTEIN SYNTHESIS; SWINE;

ALGAE; ANIMALIA; SUS SCROFA;

EID: 0031711915     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/128.9.1517     Document Type: Article

References (34)

1. Airhart, J., Vidrich, A. & Khairallah, E. A. (1974) Compartmentation of free amino acids for protein synthesis in rat liver. Biochem. J. 140: 539-548.
2. 2]tyrosine oraltracer studies at an intermediate phenylalanine intake to estimate requirements in adults. Am. J. Clin. Nutr. 65: 473-488.
3. Baumann, P. Q., Stirewalt, W. S., O'Rourke, B. D., Howard, D. & Nair, K. S. (1994) Precursor pools for protein synthesis: a stable isotope study in a swine model. Am. J. Physiol. 267: E203-E209.
4. Bergman, E. N. & Pell, J. (1986). Interorgan movement of amino acids. In: Proceedings of the XIII International Congress of Nutrition (Taylor, T. G. & Jenkins, N. K., eds.), pp. 370-374, John Libbey, London, UK.
5. 13C-algal protein to label plasma and hepatic amino acids. J. Nutr. 125: 2516-2527.
6. Biolo, G., Tessari, P., Inchiostro, S., Bruttomesso, D., Frongher, C., Sabadin, L., Fratton, M. G., Valerio, A. & Tiengo, A. (1992) Leucine and phenylalanine kinetics during mixed meal ingestion: a multiple tracer approach. Am. J. Physiol. 262: E455-E463.
7. Cayol, M., Boirie, Y., Prugnard, J., Gachon, P., Beaufrere, B. & Obled, C. (1996) Precursor pool for hepatic protein synthesis in humans: effects of tracer route infusion and dietary proteins. Am. J. Physiol. 270: E980-E987.
8. Davis, T. A., Fiorotto, M. L & Reeds, P. J. (1993) Amino acid composition of body and milk protein changes during the suckling period in rats. J. Nutr. 123: 947-956.
9. Dudley, M. A., Nichols, B. L., Rosenberger, J., Perkinson, J. S. & Reeds, P. J. (1992) Feeding status affects in vivo prosucrase-isomaltase processing in rat jejunum. J. Nutr. 122: 528-534.
10. Ebner, S., Schoknecht, P., Reeds, P. J. & Burrin, D. G. (1994) Growth and metabolism of gastrointestinal and skeletal muscle tissues in protein-malnourished neonatal pigs. Am. J. Physiol. 266: R1736-R1743.
11. Egusa, G., Brady, D.W., Grundy, S. M. & Howard, B. V. (1983) Isopropanol precipitation method for the determination of apolipoprotein B specific activity and plasma concentrations during metabolic studies of very low density lipoprotein and low density lipoprotein apolipoprotein B. J. Lipid Res. 24: 1261-1267.
12. Garlick, P. J., Millward, D. J., James, W.P.T. & Waterlow, J. C. (1975) The effect of protein deprivation and starvation on the rate of protein synthesis in tissues of the rat. Biochim. Biophys. Acta 414: 71-84.
13. 13C]leucine infused simultaneously by gut and vein. Am. J. Physiol. 260: E111-E117.
14. Hoerr, R. A., Matthews, D. E., Bier, D. M. & Young, V. R. (1993) Effects of protein restriction and acute refeeding on leucine and lysine kinetics in young men. Am. J. Physiol. 264: E567-E575.
15. Hubscher, G., West, G. R. & Brindley, D. (1965) Studies on the fractionation of muocsal homogenates from the small intestine. Biochem. J. 97: 629-642.
16. Jahoor, F. J., Burrin, D. G., Reeds, P. J. & Frazer, M. E. (1994) Measurement of plasma protein synthesis rates in infant pigs: an investigation of alternative tracer approaches. Am. J. Physiol. 267: R221-R227.
17. Korner, A. & Debro, J. R. (1956) Solubility of albumin in alcohol after precipitation by trichloracetic acid: a simplified procedure for separation of albumin. Nature (Lond.) 454: 1067 (abs.).
18. Lichtenstein, A. H., Cohn, J. S., Hachey, D. L., Millar, J. S., Ordovas, J. M. & Schaefer, E. M. (1990) Comparison of deuterated leucine, valine and lysine in the measurement of human apolipoprotein A-I and B-100 kinetics. J. Lipid. Res. 31: 1693-1701.
19. Lobley, G. E., Connell. A. & Revell, D. (1996) The importance of transmethylation reactions to methionine metabolism in sheep: effects of supplementation with creatine and choline. Br. J. Nutr. 75: 47-56.
20. 13C-labelled amino acid mixture. Br. J. Nutr. 75: 217-235.
21. Matthews, D. E., Marano, M. A. & Campbell, R. G. (1993) Splanchnic bed utilization of leucine and phenylalanine in humans. Am. J. Physiol 264: E109-E118.
22. McNurlan, M. A., Tomkins, A. M. & Garlick, P. J. (1979) The effect of starvation on the rate of protein synthesis in rat liver and small intestine. Biochem. J. 178: 373-379.
23. Mortimore, G. E., Lardeux, B. R. & Adams, C. E. (1988) Regulation of microautophagy and basal protein turnover in rat liver. J. Biol. Chem. 263: 2506-2512.
24. Parhofer, K. G., Barrett, P.H.R., Bier, D. & Schonfeld, G.(1990) Determination of kinetic parameters of apolipoprotein B metabolism using amino acids labeled with stable isotopes. J. Lipid Res. 32: 1311-1323.
25. Reeds, P. J. (1992) Isotopic measurement of protein synthesis and proteolysis in vivo. In: Current Methods in Protein Nutrition and Metabolism (Nissen, S., ed.), pp. 249-273. Academic Press, New York, NY.
26. Reeds P. J., Hachey, D. L., Patterson, B. W., Motil, K. J. & Klein, P. D. (1992) VLDL apolipoprotein B-100, a potential indicator of the isotopic labeling of the hepatic protein synthetic precursor pool in humans: studies with multiple stable isotopically labeled amino acids. J. Nutr. 122: 457-467.
27. Reeds, P. J., Wykes, L. J., Henry, J. E., Frazer, M. E., Burrin, D. G. & Jahoor, F. (1996) Enterai glutamate is almost completely metabolized in first pass by the gastrointestinal tract of infant pigs. Am. J. Physiol. 270 : E413-E418.
28. Stein, T. P., Leskiw, M. J. & Wallace, H. W. (1992) Measurement of the half-life of human plasma fibrinogen. Am. J. Physiol. 234: E504-E510.
29. Stoll, B., Burrin, D. G., Henry, J., Jahoor, F. & Reeds, P. J. (1997) Phenylalanine utilization by the gut and liver measured with intravenous and intragastric tracers in pigs. Am. J. Physiol. 274: G1811-G1919.
30. Stoll, B., Henry, J, Reeds, P. J., Yu, H., Jahoor, F. & Burrin, D. G. (1998) Catabolism dominates the first-pass intestinal metabolism of dietary essential amino acids in milk-replacer-fed piglets. J. Nutr. 128: 606-614.
31. Vidrich, A., Airhart, J., Bruno, M. K. & Khairallah, E. A. (1977) Compartmentation of free amino acids for protein biosynthesis: influence of diurnal changes in hepatic amino acid concentrations on the composition of the precursor pool charging aminoacyl-transfer ribonucleic acid. Biochem. J. 162: 257-256.
32. 15N]leucine and substrate balance studies. Am. J. Physiol. 259: E36-E51.
33. 15N]leucine kinetics in relation to enteral and parenteral amino acid supply. Am. J. Physiol. 262: E687-E694.
34. Waterlow, J. C., Garlick, P. J. & Millward, D. J. (1978) Protein Turnover in Mammalian Tissues and in the Whole Body. North Holland, Amsterdam, The Netherlands.