Poly-β-hydroxybutyrate turnover in Azorhizobium caulinodans is required for growth and affects nifA expression

Journal of Bacteriology

Volumn 180, Issue 19, 1998, Pages 5070-5076

  Mandon, Karine ^a,c   Michel Reydellet, Nathalie ^b   Encarnación, Sergio ^a   Kaminski, P Alexandre ^b   Leija, Alfonso ^a   Cevallos, Miguel A ^a   Elmerich, Claudine ^b   Mora, Jaime ^a  

^a INSTITUTO DE CIENCIAS FÍSICAS   (Mexico)

^b INSTITUT PASTEUR   (France)

^c CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

NITROGENASE; POLY(3 HYDROXYBUTYRIC ACID); SYNTHETASE;

ARTICLE; AZORHIZOBIUM CAULINODANS; BACTERIAL GROWTH; CONTROLLED STUDY; DNA SEQUENCE; ENERGY METABOLISM; ENZYME ACTIVITY; LEGUME; NITROGEN FIXATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RHIZOBIUM; STRUCTURAL GENE; SYMBIOSIS;

AZORHIZOBIUM CAULINODANS;

EID: 0031705809     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.19.5070-5076.1998     Document Type: Article

References (48)

1. Anderson, A. J., and E. A. Dawes. 1990. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54:450-472.
2. Arigoni, F., P. A. Kaminski, J. Celli, and C. Elmerich. 1992. Transcriptional analysis of the fixABCXORF1 region of Azorhizobium caulinodans suggests post-transcriptional processing of the fixABCXORF1 mRNA. Mol. Gen. Genet. 235:422-431.
3. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1987. Current protocols in molecular biology. Greene Publishing Associates/Wiley-Interscience, New York, N.Y.
4. Bergersen, F. J., M. B. Peoples and G. L. Turner. 1991. A role for PHB in bacteroids of soybean root nodules. Proc. R. Soc. Lond. B 245:59-64.
5. 2 by bacteroids from stem nodules of Sesbania rostrata. J. Gen. Microbiol. 134:1807-1810.
6. Beringer, J. E. 1974. R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol. 84:188-198.
7. Best, E. A., and R. A. Bender. 1990. Cloning of the Klebsiella aerogenes nac gene, which encodes a factor required for nitrogen regulation of the histidine utilization (hut) operons in Salmonella typhimurium. J. Bacteriol. 172:7043-7048.
8. Bravo, A., and J. Mora. 1988. Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway. J. Bacteriol. 170: 980-984.
9. Cevallos, M. A., S. Encarnación, A. Leija, Y. Mora, and J. Mora. 1996. Genetic and physiological characterization of a Rhizobium etli mutant strain unable to synthesize poly-β-hydroxybutyrate. J. Bacteriol. 178:1646-1654.
10. Denèfle, P., A. Kush, F. Norel, A. Paquelin, and C. Elmerich. 1987. Biochemical and genetic analysis of the nifHDKE region of Rhizobium ORS571. Mol. Gen. Genet. 207:280-287.
11. 2 as the sole nitrogen source. Appl. Environ. Microbiol. 45:711-713.
12. Elmerich, C., B. L. Dreyfus, G. Reysset, and J. P. Aubert. 1982. Genetic analysis of nitrogen fixation in a tropical fast-growing Rhizobium. EMBO J. 1:499-503.
13. Encarnación, S., M. Dunn, K. Willms, and J. Mora. 1995. Fermentative and aerobic metabolism in Rhizobium etli. J. Bacteriol. 177:3058-3066.
14. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154.
15. Gebhardt, C., G. L. Turner, A. H. Gibson, B. L. Dreyfus, and F. J. Bergersen. 1984. Nitrogen-fixing growth in continuous culture of a strain of Rhizobium sp. isolated from stem nodules on Sesbania rostrata. J. Gen. Microbiol. 130:843-848.
16. Goodchild, D. J. 1977. The ultrastructure of root nodules in relation to nitrogen fixation. Int. Rev. Cytol. Suppl. 6:235-288.
17. Hirsch, A. M., M. Bang, and F. M. Ausubel. 1983. Ultrastructure analysis of ineffective alfalfa nodules formed by nif::Tn5 mutants of Rhizobium meliloti. J. Bacteriol. 155:367-380.
18. Kaminski, P. A., N. Desnoues, and C. Elmerich. 1994. The expression of nifA in Azorhizobium caulinodans requires a gene product homologous to Escherichia coli HF-I, an RNA-binding protein involved in the replication of phage Q beta RNA. Proc. Natl. Acad. Sci. USA 91:4663-4667.
19. Kaminski, P. A., and C. Elmerich. 1991. Involvement of fixLJ in the regulation of nitrogen fixation in Azorhizobium caulinodans. Mol. Microbiol. 5:665-673.
20. Kaminski, P. A., and C. Elmerich. 1998. The control of Azorhizobium caulinodans nifA expression by oxygen, ammonia and by the HF-I like protein, NrfA. Mol. Microbiol. 28:603-613.
21. 2 fixation. J. Bacteriol. 178:5989-5994.
22. Kaminski, P. A., K. Mandon, F. Arigoni, N. Desnoues, and C. Elmerich. 1991. Regulation of nitrogen fixation in Azorhizobium caulinodans: identification of a fixK-like gene, a positive regulator of nifA. Mol. Microbiol. 5:1983-1991.
23. Karr, D. B., J. K. Waters, F. Suzuki, and D. W. Emerich. 1984. Enzymes of the poly-β-hydroxybutyrate and citric acid cycles of Rhizobium japonicum bacteroids. Plant Physiol. 75:1158-1162.
24. Keen, N. T., S. Tamaki, D. Kobayashi, and D. Trolinger. 1988. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene 70:191-197.
25. Kitts, C. L., and R. Ludwig. 1994. Azorhizobium caulinodans respires with at least four terminal oxidases. J. Bacteriol. 176:886-895.
26. Knauf, V. C., and E. W. Nester. 1982. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid. Plasmid 8:45-54.
27. Kokotek, W., and W. Lotz. 1989. Construction of a lacZ-kanamycin-resistance cassette, useful for site directed mutagenesis and as a promoter probe. Gene 84:467-471.
28. Law, J. H., and R. A. Slepecky. 1961. Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82:33-36.
29. Mandon, K., P. A. Kaminski, and C. Elmerich. 1994. Functional analysis of the fixNOQP region of Azorhizobium caulinodans. J. Bacteriol. 176:2560-2568.
30. Maxwell, A., and M. Gellert. 1984. The dependence of the ATPase activity of DNA gyrase. J. Biol. Chem. 259:14472-14480.
31. Metcalf, W. W., and B. L. Wanner. 1993. Construction of new β-glucuronidase cassettes for making transcriptional fusions and their use with new methods for allele replacement. Gene 129:17-25.
32. II protein in symbiotic nitrogen fixation. J. Bacteriol. 179:3580-3587.
33. Morett, E., H. M. Fischer, and H. Hennecke. 1991. Influence of oxygen on DNA binding, positive control, and stability of the Bradyrhizobium japonicum NifA regulatory protein. J. Bacteriol. 173:3478-3487.
34. N'doye, I., S. F. Debilly, J. Vasse, B. Dreyfus, and G. Truchet. 1994. Root nodulation of Sesbania rostrata. J. Bacteriol. 176:1060-1068.
35. Pawlowski, K., U. Klosse, and F. J. de Bruijn. 1991. Characterization of a novel Azorhizobium caulinodans ORS571 two-component regulatory system, ntrYX, involved in nitrogen fixation and metabolism. Mol. Gen. Genet. 231: 124-138.
36. Pawlowski, K., P. Ratet, J. Schell, and F. De Bruijn. 1987. Cloning and characterization of nifA and ntrC genes of the stem nodulating bacterium ORS571, the nitrogen fixing symbiont of Sesbania rostrata: regulation of nitrogen fixation (nif) genes in the free-living versus symbiotic state. Mol. Gen. Genet. 206:207-219.
37. Povolo, S., R. Tombolini, A. Morea, A. J. Anderson, S. Casella, and M. P. Nuti. 1994. Isolation and characterization of mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrate. Can. J. Microbiol. 40:823-829.
38. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with selectable DNA fragments. Gene 29:303-313.
39. Quandt, J., and M. F. Hynes. 1993. Versatile suicide vectors which allow direct selection for gene replacement in gram-negative bacteria. Gene 127: 15-21.
40. Ratet, P., K. Pawlowski, J. Schell, and F. J. de Bruijn. 1989. The Azorhizobium caulinodans nitrogen fixation gene nifA is controlled by the cellular nitrogen and oxygen status. Mol. Microbiol. 3:825-838.
41. Senior, P. J., and E. A. Dawes. 1973. The regulation of poly-β-hydroxybutyrate metabolism in Azotobacter beijerinckii. J. Biochem. 134:225-238.
42. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gramnegative bacteria. Bio/Technology 1:784-791.
43. Stam, H., W. De Vries, A. H. Stouthamer, and H. W. van Verseveld. 1986. Utilization of poly-β-hydroxybutyrate in free-living cultures of Rhizobium ORS571. FEMS Microbiol. Lett. 35:215-220.
44. Steinbuchel, A., and H. G. Schlegel. 1991. Physiology and molecular genetics of poly(β-hydroxy-alkanoic-acid) synthesis in Alcaligenes eutrophus. Mol. Microbiol. 5:535-542.
45. Tal, S., and Y. Okon. 1985. Production of the reserve material poly-β-hydroxybutyrate and its function in Azospirillum brasilense. Can. J. Microbiol. 31:608-613.
46. Tombolini, R., and M. P. Nuti. 1989. Poly(β-hydroxyalkanoate) biosynthesis and accumulation by different Rhizobium species. FEMS Microbiol. Lett. 60:299-304.
47. Tombolini, R., S. Povolo, A. Buson, A. Squartini, and M. P. Nuti. 1995. Poly-β-hydroxybutyrate (PHB) biosynthetic genes in Rhizobium meliloti 41. Microbiology 141:2553-2559.
48. van Workum, M., J. M. van Dooren, N. Oldenburg, D. Molenaar, P. R. Jensen, J. L. Snoep, and H. V. Westerhokk. 1996. DNA supercoiling depends on the phosphorylation potential in Escherichia coli. Mol. Microbiol. 20:351-360.