Genetic and physiological characterization of a Rhizobium etli mutant strain unable to synthesize poly-β-hydroxybutyrate

Journal of Bacteriology

Volumn 178, Issue 6, 1996, Pages 1646-1654

  Cevallos, Miguel A ^a   Encarnacion, Sergio ^a   Leija, Alfonso ^a   Mora, Yolanda ^a   Mora, Jaime ^a  

^a INSTITUTO DE CIENCIAS FÍSICAS   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

POLY(3 HYDROXYBUTYRIC ACID);

AMINO ACID SEQUENCE; ARTICLE; CITRIC ACID CYCLE; DNA SEQUENCE; GENE SEGREGATION; GENETIC ANALYSIS; NITROGEN FIXATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RHIZOBIUM; SEQUENCE ANALYSIS; STRUCTURAL GENE; SYMBIOSIS;

EID: 0029883692     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.6.1646-1654.1996     Document Type: Article

References (48)

1. Bergersen, F. J., M. B. Peoples, and G. L. Turner. 1991. A role for poly-β-hydroxybutyrate in bacteroids of soybean root nodules. Proc. R. Soc. London B 245:59-64
2. Bergersen, F. J., and G. L. Turner. 1990. Bacteroids from soybean root nodules accumulation of poly-β-hydroxybutyrate during supply of malate and succinate in relation to nitrogen fixation in flow-chamber reactions. Proc. R. Soc. London B 240:39-59.
3. Beringer, J. E. 1974 R factor transfer in Rhizobium leguminosarum. J. Gen. Microbiol 84:188-198.
4. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
5. Bravo, A., and J. Mora. 1988. Ammonium assimilation in Rhizobium phaseoli by the glutamine synthetase-glutamate synthase pathway. J Bacteriol. 170: 980-984.
6. Calderon, J., and J. Mora. 1989. Glutamine assimilation pathways in Neurospora crassa growing on glutamine as sole nitrogen and carbon source. J Gen Microbiol. 135:2699-2707.
7. Cook, A. M., and H. G. Schlegel. 1978. Metabolite concentrations in Alcaligenes eutrophus H16 and a mutant defective in poly-β-hydroxybutyrate synthesis Arch. Microbiol. 119:231-235.
8. Ditta, G., S. Stanfield, D. Corbin, and D. R. Helinski. 1980. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium mehlon. Proc. Natl. Acad. Sci. USA 77:7347-7351.
9. +-dependent malic enzyme of Rhizobium meliloti is required for symbiotic nitrogen fixation. Mol. Microbiol. 7:865-873.
10. Encarnación, S., M. Dunn, K. Willms, and J. Mora. 1995. Fermentative and aerobic metabolism in Rhizobium eth. J Bacteriol. 177:3058-3066.
11. Engelke, T., D. Jording, D. Kapp, and A. Puehler. 1989. Identification and sequence analysis of the Rhizobium melilon dctA gene encoding the C-4-dicarboxylate carrier. J. Bacteriol. 171:5551-5560.
12. Ernst, A., H. Kirschenlohr, J. Diez, and P. Boger. 1984 Glycogen content and nitrogenase activity in Anabaena variabilis. Arch. Microbiol. 140:120-125
13. Fellay, R., J. Frey, and H. Krisch. 1987 Interposon mutagenesis of soil and water bacteria, a family of DNA fragments designed for in vitro insertional mutagenesis of gram-negative bacteria. Gene 52:147-154.
14. Friedman, A. M., S. R. Long, S. E. Brown, W. J. Buikema, and F. M. Ausubel. 1982. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhtzobntm mutants. Gene 18:289-296.
15. 13C-NMR investigation Eur. J. Biochem. 207:155-162
16. Goodchild, D. J. 1977. The ultrastructure of root nodules in relation to nitrogen fixation, p. 235-288. In G. H Bourne, J. F. Danielli, and K. W. Jeon (ed.), International review of cytology. Academic Press, New York
17. Hanahan, D. 1983. Studies of transformation of E. coli with plasmids. J. Mol. Biol. 166:557-560
18. Hansen, R. G., and U. Henning. 1966. Regulation of pyruvate dehydrogenase activity in Escherichia coli K12. Biochim. Biophys. Acta 122:355-358.
19. Hardarson, G. 1993. Methods for enhancing symbiotic nitrogen fixation. Plant Soil 152:1-17.
20. Hirsch, A. M., M. Bang, and F. M. Ausubel. 1983. Ultrastructural analysis of ineffective alfalfa nodules formed by nif:Tr5 mutants of Rhizobium meliloti. J. Bacteriol. 155:367-380.
21. Huisman, G. W., E. Wonink, R. Meima, B. Kazemier, P. Terpstra, and B. Witholt. 1991. Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas oleovorans. Identification and sequences of genes and function of the encoded proteins in the synthesis and degradation of PHA J. Biol. Chem. 266:2191-2198.
22. Hustede, E., A. Steinbuechel, and H. G. Schlegel. 1993. Relationship between the photoproduction of hydrogen and the accumulation of PHB in non-sulphur purple bacteria. Appl. Microbiol. Biotechnol. 39:87-93.
23. Karr, D. B., J. K. Waters, F. Suzuki, and D. W. Emerich. 1984. Enzymes of the poly-β-hydroxybutyrate and citric acid cycles of Rhizobium japonicum bacteroids. Plant Physiol. 75:1158-1162.
24. Law, J. H., and R. A. Slepecky. 1961. Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82:33-36.
25. Lowry, O. H., N. J. Rosehrongh, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem 193:265-275.
26. Masterson, C. L., and P. M. Murphy. 1980. The acetylene reduction technique, p. 8-83. In N S. Subba Rao (ed.), Recent advances in biological nitrogen fixation. Edward Arnold, London.
27. McDermott, T. R., S. M. Griffith, C. P. Vance, and P. H. Graham. 1989. Carbon metabolism in Bradyrhizobium japonicum bacteroids. FEMS Microbiol. Rev 63:327-340.
28. Mora, J., R. Salceda, and S. Sánchez. 1972. Regulation of arginase activity by intermediates of the arginine biosynthetic pathway in Neurospara crassa. J. Bacteriol. 110:870-877.
29. Ndoye, I., S. F. Debilly, J. Vasse, B. Dreyfus, and G. Truchet. 1994 Root modulation of Sesbania rostrata. J Bacteriol. 176:1060-1068.
30. Noel, K. D., A. Sánchez, L. Fernández, J. Leemans, and M. A. Cevallos. 1984. Rhizobium phaseoli symbiotic mutants with transposon Tn5 insertions J. Bacteriol. 158:148-155.
31. Peoples, O. P., and A. J. Sinskey. 1989. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16: identification and characterization of the PHB polymerase gene (phbC). J Biol. Chem 264:15298-15303.
32. Povolo, S., R. Tombolini, A. Morea, A. J. Anderson, S. Casella, and M. P. Nuti. 1994. Isolation and characterization of mutants of Rhizobium meliloti unable to synthesize poly-β-hydroxybutyrate. Can. J. Microbiol. 40:823-829.
33. Prentki, P., and H. M. Krisch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
34. Quinto, C., H. Oe la Vega, M. Flores, T. Ballado, G. Soberón, and R. Palacios. 1982. Reiteration of nitrogen fixation gene sequences in Rhizobium phaseoli. Nature (London) 299:724-726.
35. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J. Cell Biol 17:208-213.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
37. Schlegel, H. G., R. Lafferty, and I. Krauss. 1970 The isolation of mutants not accumulating poly-β-hydroxybutyric acid. Arch. Microbiol. 71:283-294.
38. - bacteria using a genetically modified sacRB gene as a positive selection marker. Appl. Microbiol. Biotechnol. 38:615-618
39. Smith, C. M., J. Bryla, and J. R. Williamson. 1974. Regulation of mitochondrial α-ketoglutarate metabolism by product inhibition at β-ketoglutarate dehydrogenase. J. Biol. Chem. 249:1497-1505.
40. Stam, H., W. Devries, A. H. Stouthamer, and H. W. Vanverseveld. 1986. Utilization of poly-β-hydroxybutyratc in free-living cultures of Rhizobium ORSS71. FEMS Microbiol. Lett. 35:215-220.
41. Steinbuchel, A., and H. G. Schlegel. 1989. Excretion of pyruvate by mutants of Alcaligenes eutrophus, which are impaired in the accumulation of poly(β-hydroxybutyric acid) (PHB) under conditions permitting synthesis of PHB. Appl. Microbiol. Biotechnol. 31:168-175.
42. Steinbuchel, A., and H. G. Schlegel. 1991. Physiology and molecular genetics of poly(b-hydroxy-alkanoic acid) synthesis in Alcaligenes eutrophus. Mol. Microbiol 5:535-542.
43. Tombolini, R., and M. P. Nuti. 1989. Poly (β-hydroxyalkanoate) biosynthesis and accumulation by different Rhizobium species. FEMS Microbiol. Lett 60: 299-304.
44. Tsien, H. C., and E. L. Schmidt. 1977. Polarity in the exponential-phase Rhtzohium japonicum cell. Can J. Microbiol. 23:1274-1284.
45. Vollbrecht, D., M. A. El Nawawy, and H. G. Schlegel. 1978. Excretion of metabolites by hydrogen bacteria. I. Autotrophic and heterotrophic fermentations. Eur. J. Appl. Microbiol. Biotechnol. 6:145-155.
46. Vollbrecht, D., and H. G. Schlegel. 1979. Excretion of metabolites by hydrogen bacteria. III. D(-)-3-Hydroxybutanoate. Eur. J. Appl. Microbiol. Biotechnol. 7:259-266.
47. Wild, E. 1962. Untersuchungen über Wachstum und Speicherstoffsynthase von Hydrogenomonas. Arch. Mikrobiol. 43:109-137.
48. Wong, P. P., and H. J. Evans. 1971. Poly-β-hydroxybutyrate utilization by soybean (Glycine max Merr.) nodules and assessment of its role in maintenance of nitrogenase activity. Plant Physiol. 47:750-755.