Are intraepithelial lymphocytes in celiac mucosa responsible for inducing programmed cell death (apoptosis) in enterocytes? Histochemical demonstration of perforins in cytoplasmic granules of intraepithelial lymphocytes

Journal of Pediatric Gastroenterology and Nutrition

Volumn 27, Issue 4, 1998, Pages 393-396

  Shiner, Margot ^a   Eran, Maya ^b   Freier, Serem ^b   Faber, Joseph ^b   Branski, David ^b,c  

^a TEL AVIV UNIVERSITY   (Israel)

^b HEBREW UNIVERSITY   (Israel)

^c SHAARE ZEDEK MEDICAL CENTER   (Israel)

Author keywords

Apoptosis; Celiac disease; Granzymes; Intraepithelial lymphocytes; Perforins

Indexed keywords

PERFORIN; SERINE PROTEINASE;

APOPTOSIS; ARTICLE; CELIAC DISEASE; CELL GRANULE; CHILD; CLINICAL ARTICLE; CONTROLLED STUDY; CYTOTOXIC LYMPHOCYTE; HISTOCHEMISTRY; HUMAN; HUMAN CELL; HUMAN TISSUE; INTESTINE CELL; INTESTINE MUCOSA; MONONUCLEAR CELL; PRIORITY JOURNAL;

APOPTOSIS; CELIAC DISEASE; CHILD; CYTOPLASMIC GRANULES; GRANZYMES; HISTOCYTOCHEMISTRY; HUMANS; INTESTINAL MUCOSA; LYMPHOCYTES; LYSINE; MEMBRANE GLYCOPROTEINS; PORE FORMING CYTOTOXIC PROTEINS; SERINE ENDOPEPTIDASES;

EID: 0031695258     PISSN: 02772116     EISSN: None     Source Type: Journal    
DOI: 10.1097/00005176-199810000-00004     Document Type: Article

References (27)

1. Wyllie AH, Kerr JR, Currie AR. Cell death: The significance of apoptosis. Int Rev Cytol 1980;68:251-306.
2. Podack ER. Execution and suicide: Cytotoxic lymphocytes enforce Draconian laws through separate molecular pathways. Curr Opin Immunol 1995;7:11-6.
3. Cerf-Bensussan N, Brousse N, Jarry A, et al. Role of in vivo activated T cells in the mechanisms of villous atrophy in humans: Study of allograft rejection. Digestion 1990;46:297-301.
4. Viney J, MacDonald TT, Spencer J. Gamma/delta T cells in the gut epithelium. Gut 1990;31:841-4.
5. Hadziselimovic F, Emmons LR, Schaub U, Signer E, Buergin-Wolff A, Krstic R. Occurrence of large granular lymphocytes and natural killer cells in the epithelium of the gut distinguishes two different celiac diseases. Gut 1992;33:767-72.
6. Kramer MD, Ruth U, Simon H-G, Simon MM. Expression of cytoplasmic granules with T cell-associated serine proteinase-1 activity in Ly-2+ (CDS+) T lymphocytes responding to lymphocytic choriomeningitis virus in vivo. Eur J Immunol 89;19:151-6.
7. Lebenthal E, Branski D. Childhood celiac disease: An appraisal. J Pediatr 1981;98:681-90.
8. Ferguson A, Murray D. Quantitation of intraepithelial lymphocytes in human jejunum. Gut 1971;12:988-94.
9. Pasternack MS, Verret CR, Liu MA, Eisen HN. Serine esterase in cytolytic T lymphocytes. Nature 1986;322:740-3.
10. Berke G. The binding and lysis of target cells by cytotoxic lymphocytes: Molecular and cellular aspects. Annu Rev Immunol 1994; 12:735-7.
11. Lowin B, Hahne M, Mattmann C, Tschopp J. Cytolytic T-cell cytotoxicity is mediated through perforin and as lytic pathways Nature 1994;370:650-2.
12. Iwanaga T, Han H, Hoshi O, Takahashi-Iwanaga H, Fujita T. Perforin-containing lymphocytes and their ultrastructure in the intestinal mucosa with special reference to species difference between the guinea pig and rat. Biomed Res 1994;15:67-76.
13. Kato Y, Yokochi T, Maeda K, et al. Natural killer (NK)-like cytotoxicity of murine intraepithelial lymphocytes in the small intestine (iIEL) and the effect of the serine proteases. Microbiol Immunol 1995;39:291-4.
14. Shibahara T, Sato N, Waguri S, et al. The fate of effete epithelial cells at the villus tips of the human small intestine. Arch Histol Cytol 1995;58:205-19.
15. Takahashi-Iwanaga H, Iwanaga T, Sakamoto Y, Fujita T. Ultrastructural and time-lapse observations of intraepithelial lymphocytes in the small intestine of the guinea pig: Their possible role in the removal of effete enterocytes. Cell Tissue Res 1995;280:491-7.
16. Trapani JA, Smyth MJ, Apostolidis VA, Dawson M, Browne KA. Granule serine proteases are normal nuclear constituents of natural killer cells. J Biochem 1994;269:18359-65.
17. Renner C, Held G, Ohnesorge S, Bauer S, Gerlach K, Pfitzenmeier M. Role of perforin, granzymes and the proliferative state of the target cells in apoptosis and necrosis mediated by bispecificantibody-activated cytotoxic T cells. Cancer Immunol Immunother 1997;44:70-6.
18. Nakajima H, Henkart PA. Cytotoxic lymphocytes granules trigger a target cell internal disintegration pathway leading to cytolysis and DNA breakdown. J Immunol 1994;152:1057-63.
19. Kato Y, Yokochi T, Sasaki K, Kawamoto Y, Tsuji T, Miyama A. The major component of Na-CBZ-L-Lysine thiobenzyl ester (BLT)-specific proteases in cytoplasmic granules of murine intraepithelial lymphocytes is granzyme A. Immunobiol 1997;196:465-74.
20. Vettel U, Brunner G, Bar-shavit R, Vlodavsky I, Kramer MD. Charge-dependent binding of granzyme A (MTSP-1) to basement membranes. Eur J Immunol 1993;23:279-82.
21. Shiner M. Ultrastructure of the small intestinal mucosa. Berlin, Heidelberg, New York: Springer Verlag 1983:61-78.
22. Wagner L, Wiesholzer M, Worman CP, Lang G, Base W. BLT-esterase in infectious mononucleosis. Clin Exp Immunol 1995;100: 223-6.
23. Wagner L, Base W, Wiesholzer M, Sexl V, Bognar H, Worman CP. Detection of BLT substrate-specific proteases in individual human peripheral blood leucocytes and bone marrow cells. J Immunol Methods 1991;142:147-55.
24. Cerf-Bensussan N, Guy-Grand D, Griscelli C. Intraepithelial lymphocytes of human gut: Isolation, characterisation and study of natural killer activity. Gut 1985;26:81-8.
25. Oberhuber G, Vogelsang H, Stoke M, Muthenthaler S, Kummer AJ, Radaszkiewicz T. Evidence that intestinal intraepithelial lymphocytes are activated cytotoxic T cells in celiac disease but not in giardiasis. Am J Pathol 1996;148:1351-7.
26. Moss SF, Attia L, Scholes JV, Walters JRF, Holt PR. Increased small intestinal apoptosis in coeliac disease. Gut 1996;39:811-7.
27. Iwanaga T, Han H, Adachi K, Fujita T. A novel mechanism for disposing of effete epithelial cells in the small intestine of guinea pigs. Gastroenterology 1993;105:1089-97.