Cre expression in primary spermatocytes: A tool for genetic engineering of the germ line

Molecular Reproduction and Development

Volumn 51, Issue 3, 1998, Pages 274-280

  Vidal, Frédérique ^a   Sage, Julien ^a   Cuzin, François ^a   Rassoulzadegan, Minoo ^a  

^a INSERM   (France)

Author keywords

Genomic rearrangements; LoxP excision; Recombination; Transgenic mice

Indexed keywords

MESSENGER RNA; PLASMID DNA;

ANIMAL CELL; ARTICLE; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENOME; GENOTYPE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPERM; TRANSGENIC MOUSE;

ANIMALS; GENE EXPRESSION; GENES, REPORTER; GENETIC ENGINEERING; INTEGRASES; MALE; MICE; MICE, TRANSGENIC; NUCLEAR PROTEINS; SPERMATOCYTES; VIRAL PROTEINS;

ANIMALIA; MUS MUSCULUS; RODENTIA;

EID: 0031695118     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-2795(199811)51:3<274::AID-MRD6>3.0.CO;2-M     Document Type: Article

References (20)

1. Akagi K, Sandig V, Vooijs M, Van der Valk M, Giovannini M, Strauss M, Berns A (1997): Cre-mediated somatic site-specific recombination in mice. Nucleic Acids Res 25:1766-1773.
2. Besmer P, Manovo K, Duttlinger R, Huang EJ, Packer A, Gyssler C, Bachvarova RF (1993): The Kit-ligand (steel factor) and its receptor c-kit/W: Pleiotropic roles in gametogenesis and melanogenesis. In P Ingham, A Brown, A Martinez-Arias (eds): "Development 1993: Supplements." Cambridge: The Company of Biologists Ltd., pp 125-137.
3. Brocard J, Warot X, Wendling O, Messaddeq N, Vonesch JL, Chambon P, Metzger D (1997): Spatio-temporally controlled site-specific somatic mutagenesis in the mouse. Proc Natl Acad Sci USA 94:14559-14563.
4. Chomczynski P, Sacchi N (1987): Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction. Anal Bioch 162:152-159.
5. Feil R, Brocard J, Mascrez B, LeMeur M, Metzger D, Chambon P (1996): Ligand-activated site-specific recombination in mice. Proc Natl Acad Sci USA 93:10887-10890.
6. Friedrich G, Soriano P (1991): Promoter traps in embryonic stem cells: A genetic screen to identify and mutate developmental genes in mice. Genes Dev 5:1513-1523.
7. Gowen LC, Johnson BL, Latour AM, Sulik KK, Koller BH (1996): Brcal deficiency results in early embryonic lethality characterized by neuroepithelial abnormalities. Nat Genet 12:191-194.
8. Gu H, Marth JD, Orban PC, Mossmann H, Rajewsky K (1994): Deletion of a DNA polymerase beta gene segment in T cells using cell type-specific gene targeting. Science 265:103-106.
9. Hogan B, Costantini F, Lacy L (1986): "Manipulating the mouse embryo: A laboratory manual." Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
10. Kühn R, Schwenk F, Aguet M, Rajewski K (1995): Inducible gene targeting in mice. Science 269:1427-1429.
11. Meuwissen RLJ, Offenberg HH, Dietrich AJJ, Riesewijk A, van Iersel M, Heyting C (1992): A coiled-coiled protein specific for synapsed regions of meiotic prophase chromosomes. EMBO J 11:5091-5100.
12. O'Gorman S, Dagenais NA, Qian M, Marchuk Y (1997): Protamine-Cre recombinase trans-genes efficiently recombine target sequences in the male germ line of mice, but not in embryonic stem cells. Proc Natl Acad Sci USA 94:14602-14607.
13. Porter A (1998): Controlling your losses: Conditional gene silencing in mammals. Trends Genet 14:73-79.
14. Sage J, Yuan L, Martin L, Mattei M, Guénet J-L, Liu J-G, Hoög C, Rassoulzadegan M, Cuzin F (1997): The Scp 1 loci of the mouse genome: Successive retropositions of a meiotic gene during the recent evolution of the genus. Genomics 44:118-126.
15. Sambrook J, Fritsch EF, Maniatis T (1989): "Molecular cloning: A laboratory manual." Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
16. Sauer B, Henderson N (1989): Cre-stimulated recombination at loxP-containing DNA sequences placed into the mammalian genome. Nucleic Acids Res 17:147-161.
17. Sauer B, Henderson N (1990): Targeted insertion of exogenous DNA into the eukaryotic genome by the Cre recombinase. New Biol 2:441-449.
18. Tsuzuki T, Fujii Y, Sakumi K, Tominaga Y, Nakao K, Sekiguchi M, Matsushiro A, Yoshimura Y, Morita T (1996): Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc Natl Acad Sci USA 93:6236-6240.
19. Wagner KU, Wall RJ, St Onge L, Gruss P, Wynshaw BA, Garrett L, Li M, Furth PA, Hennighausen L (1997): Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res 25:4323-4330.
20. Zabludoff SD, Wright WW, Harshman K, Wold BJ (1996): BRCA1 mRNA is expressed highly during meiosis and spermiogenesis but not during mitosis of male germ cells. Oncogene 13:649-653.