Molecular cloning and functional characterization of a rat somatostatin sst(2(b)) receptor splice variant

British Journal of Pharmacology

Volumn 125, Issue 1, 1998, Pages 209-217

  Schindler, Marcus ^a   Kidd, Emma J ^a   Carruthers, Alan M ^a   Wyatt, Mark A ^a   Jarvie, Emma M ^a   Sellers, Lynda A ^a   Feniuk, Wasyl ^a   Humphrey, Patrick P A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

Adenylate cyclase; Cloning; Microphysiometry; RT PCR; Somatostatin receptors; Splice variants; sst(2(a)); sst(2(b))

Indexed keywords

COMPLEMENTARY DNA; CYCLIC AMP; FORSKOLIN; GUANINE NUCLEOTIDE BINDING PROTEIN; PERTUSSIS TOXIN; RECEPTOR SUBTYPE; SOMATOSTATIN RECEPTOR;

ACIDIFICATION; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHO CELL; CONTROLLED STUDY; DESENSITIZATION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RAT; STOMACH MUCOSA;

ADENYLATE CYCLASE; ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CHO CELLS; CLONING, MOLECULAR; CRICETINAE; DNA; GASTRIC MUCOSA; MOLECULAR SEQUENCE DATA; RATS; RECEPTORS, SOMATOSTATIN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE ALIGNMENT;

EID: 0031689673     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0702064     Document Type: Article

References (30)

1. BROWN, B.L., ALBANO, J.D., EKINS, R.P. & SGHERZI, A.M. (1971). A simple and sensitive saturation assay method for the measurement of adenosine 3′ : 5′-cyclic monophosphate. Biochem. J., 121, 561-562.
2. CASTRO, S.W., FENIUK, W. & HUMPHREY, P.P.A. (1996). Differences in the operational characteristics of the human recombinant somatostatin receptor types, sst1 and sst2, in mouse fibroblast (Ltk-) cells. Br. J. Pharmacol., 117, 639-646.
3. CHOMCZYNSKI, P. & SACCHI, N. (1987). Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156-159.
4. DORNAUD, P., GU, Y.Z., SCHONNBRUNN, A., MAZELLA, J., TANNENBAUM, G.S. & BEAUDET, A. (1996). Localization of the somatostatin receptor sst2A in rat brain using a specific antipeptide antibody. J. Neurosci., 16, 4468-4478.
5. DRUEY, K.M., BLUMER, K.J., KANG, V.H. & KEHRL, J.H. (1996). Inhibition of G-protein-mediated MAP kinase activation by a new mammalian gene family. Nature, 379, 742-746.
6. EPELBAUM, J. (1986). Somatostatin in the central nervous system: physiology and pathological modifications. Prog. Neurobiol., 27, 63-100.
7. 1α3. J. Biol. Chem., 272, 7770-7776.
8. GU, W.Z., BROWN, P.J., LOOSE-MITCHELL, D.S., STORK, P.J.S. & SCHONBRUNN, A. (1995). Development and use of a receptor antibody to characterize the interaction between somatostatin receptor subtype 1 and G proteins. Mol. Pharmacol., 48, 1004-1014.
9. HOYER, D., BELL, G.I., BERELOWITZ, M., EPELBAUM, J., FENIUK, W., HUMPHREY, P.P.A., O'CARROLL, A-M., PATEL, Y.C., SCHONBRUNN, A., TAYLOR, J.E. & REISINE, T. (1995). Classification and nomenclature of somatostatin receptors. Trends Pharmacol. Sci., 16, 86-88.
10. HUNYADY, B., HIPKIN, R.W., SCHONBRUNN, A. & MEZEY, E. (1997). Immunohistochemical localization of somatostatin receptor sst2A in the rat pancreas. Endocrinology, 138, 2632-2635.
11. 2(b) receptor splice variant in the rat gastric mucosa. Br. J. Pharmacol., 123, 125P.
12. KLUXEN, F-W., BRUNS, C. & LÜBBERT, H. (1992). Expression cloning of a rat brain somatostatin receptor cDNA. Proc. Natl. Acad. Sci. (U.S.A.), 89, 4618-4622.
13. (o)α selectively associate with the cloned somatostatin receptor subtype SSTR2. J. Biol. Chem., 268, 587-590.
14. LIGGETT, S.B., OSTROWSKI, J., CHESNUT, L.C., KUROSE, H., RAYMOND, J.R., CARON, M.G. & LEFKOWITZ, R.J. (1992). Sites in the third intracellular loop of the alpha 2A-adrenergic receptor confer short term agonist-promoted desensitization. Evidence for a receptor kinase-mediated mechanism J. Biol. Chem., 267, 4740-4746.
15. LLOYD, K.C.K., WANG, J., AURANG, K., GRONHED, P., COY, D.H. & WALSH, J.H. (1995). Activation of somatostatin receptor subtype 2 inhibits acid secretion in rats. Am. J. Physiol., 268, G102-108.
16. MILLIGAN, G. (1993). Mechanisms of multifunctional signalling by G protein-linked receptors. Trends Pharmacol. Sci., 14, 239-244.
17. PALMER, T.M., BENOVIC, J.L. & STILES, G.L. (1995). Agonist-dependent phosphorylation and desensitization of the rat A3 adenosine receptor. Evidence for a G-protein-coupled receptor kinase-mediated mechanism. J. Biol. Chem., 270, 29607-29613.
18. PATEL, Y.C., GREENWOOD, M., KENT, G., PANETTA, R. & SRIKANT, C.B. (1993). Multiple gene transcripts of the somatostatin receptor SSTR2: tissue selective distribution and cAMP regulation. Biochem. Biophys. Res. Comm., 192, 288-294.
19. REISINE, T. & BELL, G.I. (1995). Molecular properties of somatostatin receptors. Neuroscience, 67, 777-790.
20. REISINE, T., KONG, H.Y., RAYNOR, K., YANO, H., TAKEDA, J., YASUDA, K. & BELL, G.I. (1993). Splice variant of the somatostatin receptor 2 subtype, somatostatin receptor 2B, couples to adenylyl cyclase. Mol. Pharmacol., 44, 1008-1015.
21. SAMBROOK, J., FRITSCH, E.F. & MANIATIS, T. (1989). Molecular cloning - a laboratory manual, Cold Spring Harbor Laboratory Press, New York, U.S.A.
22. SANGER, F., NIKLEN, S. & SOULSON, A.R. (1977). DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. (U.S.A.), 74, 5463-5467.
23. SCHINDLER, M., HUMPHREY, P.P.A. & EMSON, P.C. (1996). Somatostatin receptors in the central nervous system. Progr. Neurobiol., 50, 9-47.
24. 2(a) receptor in rat brain and spinal cord. Neuroscience, 76, 225-240.
25. 2B receptors. Br. J. Pharmacol., 123, 110P.
26. VANETTI, M., KOUBA, M., VOGT, G. & HÖLLT, V. (1992). Cloning and expression of a novel mouse somatostatin receptor (SSTR2B). FEBS Lett., 311, 290-294.
27. VANETTI, M., VOGT, G. & HÖLLT, V. (1993). The two isoforms of the mouse somatostatin receptor (mSSTR2A and mSSTR2B) differ in coupling efficiency to adenylate cyclase and in agonist-induced desensitisation. FEBS Lett., 331, 260-266.
28. VANETTI, M., ZIÓLKOWSKA, B., WANG, X., HORN, G. & HÖLLT, V. (1994). mRNA distribution of two isoforms of somatostatin receptor 2 (mSSTR2A and mSSTR2B) in mouse brain. Mol. Brain Res., 27, 45-50.
29. VON ZASTROW, M. & KOBILKA, B.K. (1992). Ligand-regulated internalization and recycling of human beta 2-adrenergic receptors between the plasma membrane and endosomes containing transferrin receptors. J. Biol. Chem., 267, 3530-3538.
30. 2 receptor-mediated inhibition of parietal cell function in rat isolated gastric mucosa. Br. J. Pharmacol., 119, 905-910.