Peak height pattern in dichloro-rhodamine and energy transfer dye termintor sequencing

BioTechniques

Volumn 25, Issue 3, 1998, Pages 406-414

  Zakeri, H ^a   Amparo, G ^a,b   Chen, S M ^a,b   Spurgeon, S ^a,b   Kwok, P Y ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b APPLIED BIOSYSTEMS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DYE; RHODAMINE;

ARTICLE; AUTOANALYSIS; COMPUTER PROGRAM; DNA SEQUENCE; ENERGY TRANSFER; HUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION;

EID: 0031687519     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98253st01     Document Type: Article

References (7)

1. Kwok, P.-Y., C. Carlson, T.D. Yager, W. Ankener and D.A. Nickerson. 1994. Comparative analysis of human DNA variations by fluorescence-based sequencing of PCR products. Genomics 23:138-144.
2. Lee, L.G., S.L. Spurgeon, C.R. Heiner, S.C. Benson, B.B. Rosenblum, S.M. Menchen, R.J. Graham, A. Constantinescu, K.G. Upadhya and J.M. Cassel. 1997. New energy transfer dyes for DNA sequencing. Nucleic Acids Res. 25:2816-2822.
3. Parker, L.T., Q. Deng., H. Zakeri, C. Carlson, D.A. Nickerson and P.-Y. Kwok. 1995. Peak height variations in automated sequencing of PCR products using Taq dye-terminator chemistry. BioTechniques 19:116-121.
4. Parker, L.T., H. Zakeri, Q. Deng, S. Spurgeon, P.-Y. Kwok and D.A. Nickerson. 1996. AmpliTaq® DNA Polymerase, FS Dye-Terminator sequencing: analysis of peak height patterns. BioTechniques 21:694-699.
5. Rosenblum, B.B., L.G. Lee, S.L. Spurgeon, S.H. Khan, S.M. Menchen, C.R. Heiner and S.M. Chen. 1997. New dye-labeled terminators for improved DNA sequencing patterns. Nucleic Acids Res. 25:4500-4504.
6. Rosenthal, A. and D.S. Charnock-Jones. 1992. New protocols for DNA sequencing with dye terminators, DNA Seq. 3:61-64.
7. Tracy, T.E. and L.S. Mulcahy. 1991. A simple method for direct automated sequencing of PCR fragments. BioTechniques 11:68-75.