AmpliTaq® DNA polymerase, FS dye-terminator sequencing: Analysis of peak height patterns

BioTechniques

Volumn 21, Issue 4, 1996, Pages 694-699

  Parker, L T ^c   Zakeri, H ^c   Deng, Q ^a   Spurgeon, S ^b   Kwok, P Y ^c   Nickerson, D A ^a  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b APPLIED BIOSYSTEMS   (United States)

^c WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; DNA DIRECTED DNA POLYMERASE; TAQ POLYMERASE;

ANALYTIC METHOD; ANALYTICAL EQUIPMENT; ARTICLE; BINDING SITE; DNA DETERMINATION; DNA POLYMORPHISM; DNA SEQUENCE; ENZYME SYNTHESIS; TRANSCRIPTION TERMINATION; POLYMERASE CHAIN REACTION;

DNA-DIRECTED DNA POLYMERASE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TAQ POLYMERASE;

EID: 0029658425     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96214rr02     Document Type: Article

References (8)

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2. Kwok, P.Y., C. Carlson, T.D. Yager, W. Ankener and D.A. Nickerson. 1994. Comparative analysis of human DNA variations by fluorescence-based sequencing of PCR products. Genomics 23:138-144.
3. Lee, L.G., C.R. Connell, S.L. Woo, R.D. Cheng, B.F. McArdle, C.W. Fuller, N.D. Halloran and R.K. Wilson. 1992. DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments. Nucleic Acids Res. 20:2471-2483.
4. Parker, L.T., Q. Deng., H. Zakeri, C. Carlson, D.A. Nickerson and P.-Y. Kwok. 1995. Peak height variations in automated sequencing of PCR products using Taq dye-terminator chemistry. BioTechniques 19:116-121.
5. Reeve, M.A. and C.W. Fuller. 1995. A novel thermostable polymerase for DNA sequencing. Nature 376:796-797.
6. Rosenthal, A. and D.S. Charnock-Jones. 1992. New protocols for DNA sequencing with dye terminators. DNA Seq. 3:61-64.
7. Tabor, S. and C.C. Richardson. 1995. A single residue in DNA polymerases of the Escherichia coli DNA polymerase I family is critical for distinguishing between deoxy- and dideoxyribonucleotides. Proc. Natl. Acad. Sci. USA 92:6339-6343.
8. Tracy, T.E. and L.S. Mulcahy. 1991. A simple method for direct automated sequencing of PCR fragments. BioTechniques 11:68-75.