Intracellular localization of poliovirus plus- and minus-strand RNA visualized by strand-specific fluorescent in situ hybridization

Journal of Virology

Volumn 72, Issue 11, 1998, Pages 8578-8585

  Bolten, Roger ^a,c   Egger, Denise ^a   Gosert, Rainer ^a   Schaub, Gabriela ^a   Landmann, Lukas ^b   Bienz, Kurt ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITY OF BASEL   (Switzerland)

^c Drossapharm   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS PROTEIN; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; FLUORESCENCE IN SITU HYBRIDIZATION; GOLGI COMPLEX; HEP 2 CELL; HUMAN; HUMAN CELL; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RNA PROBE; RNA SYNTHESIS; VIRUS MORPHOLOGY; VIRUS REPLICATION;

EID: 0031687438     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.72.11.8578-8585.1998     Document Type: Article

References (50)

1. Aldabe, R., A. Barco, and L. Carrasco. 1996. Membrane permeabilization by poliovirus proteins 2B and 2BC. J. Biol. Chem. 271:23134-23137.
2. Aldabe, R., and L. Carrasco. 1995. Induction of membrane proliferation by poliovirus proteins 2C and 2BC. Biochem. Biophys. Res. Commun. 206:64-76.
3. Andino, R., G. E. Rieckhof, and D. Baltimore. 1990. A functional ribonucleo-protein complex forms around the 5′ end of poliovirus RNA. Cell 63:369-380.
4. Banerjee, R., A. Echeverri, and A. Dasgupta. 1997. Poliovirus-encoded 2C polypeptide specifically binds to the 3′-terminal sequences of viral negative-strand RNA. J. Virol. 71:9570-9578.
5. Baron, M. H., and D. Baltimore. 1982. In vitro copying of viral positive strand RNA by poliovirus replicase. Characterization of the reaction and its products. J. Biol. Chem. 257:12359-12366.
6. Barton, D. J., E. P. Black, and J. B. Flanegan. 1995. Complete replication of poliovirus in vitro: preinitiation RNA replication complexes require soluble cellular factors for the synthesis of VPg-linked RNA. J. Virol. 69:5516-5527.
7. Barton, D. J., and J. B. Flanegan. 1997. Synchronous replication of poliovirus RNA: initiation of negative-strand RNA synthesis requires the guanidine-inhibited activity of protein 2C. J. Virol. 71:8482-8489.
8. Bienz, K., and D. Egger. 1995. Immunocytochemistry and in situ hybridization in the electron microscope: combined application in the study of virus-infected cells. Histochem. Cell Biol. 103:325-338.
9. Bienz, K., D. Egger, and L. Pasamontes. 1987. Association of polioviral proteins of the P2 genomic region with the viral replication complex and virus-induced membrane synthesis as visualized by electron microscopic immunocytochemistry and autoradiography. Virology 160:220-226.
10. Bienz, K., D. Egger, and T. Pfister. 1994. Characteristics of the poliovirus replication complex. Arch. Virol. Suppl. 9:147-157.
11. Bienz, K., D. Egger, T. Pfister, and M. Troxler. 1992. Structural and functional characterization of the poliovirus replication complex. J. Virol. 66: 2740-2747.
12. Bienz, K., D. Egger, Y. Rasser, and W. Bossart. 1980. Kinetics and location of poliovirus macromolecular synthesis in correlation to virus-induced cytopathology. Virology 100:390-399.
13. Bienz, K., D. Egger, Y. Rasser, and W. Bossart. 1983. Intracellular distribution of poliovirus proteins and the induction of virus-specific cytoplasmic structures. Virology 131:39-48.
14. Bienz, K., D. Egger, M. Troxler, and L. Pasamontes. 1990. Structural organization of poliovirus RNA replication is mediated by viral proteins of P2 genomic region. J. Virol. 64:1156-1163.
15. Caliguiri, L. A., and I. Tamm. 1970. The role of cytoplasmic membranes in poliovirus biosynthesis. Virology 42:100-111.
16. Cao, X. M., R. J. Kuhn, and E. Wimmer. 1993. Replication of poliovirus RNA containing two VPg coding sequences leads to a specific deletion event. J. Virol. 67:5572-5578.
17. Cho, M. W., N. Teterina, D. Egger, K. Bienz, and E. Ehrenfeld. 1994. Membrane rearrangement and vesicle induction by recombinant poliovirus 2C and 2BC in human cells. Virology 202:129-145.
18. Cox, K. H., D. V. DeLeon, L. M. Angerer, and R. C. Angerer. 1984. Detection of mRNAs in sea urchin embryos by in situ hybridization using asymmetric RNA probes. Dev. Biol. 101:485-502.
19. Dales, S., H. J. Eggers, I. Tamm, and G. E. Palade. 1965. Electron microscopic study of the formation of poliovirus. Virology 26:379-389.
20. Egger, D., L. Pasamontes, R. Bolten, V. Boyko, and K. Bienz. 1996. Reversible dissociation of the poliovirus replication complex: functions and interactions of its components in viral RNA synthesis. J. Virol. 70:8675-8683.
21. Etchison, D., and E. Ehrenfeld. 1981. Comparison of replication complexes synthesizing poliovirus RNA. Virology 111:33-46.
22. Flanegan, J. B., and D. Baltimore. 1979. Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide. J. Virol. 29: 352-360.
23. Girard, M. 1969. In vitro synthesis of poliovirus ribonucleic acid: role of the replicative intermediate. J. Virol. 3:376-384.
24. Guinea, R., and L. Carrasco. 1990. Phospholipid biosynthesis and poliovirus genome replication, two coupled phenomena. EMBO J. 9:2011-2016.
25. pro with the 5′ and 3′ termini of the poliovirus genome-identification of viral and cellular cofactors needed for efficient binding. J. Biol. Chem. 269:27004-27014.
26. Li, J. P., and D. Baltimore. 1988. Isolation of poliovirus 2C mutants defective in viral RNA synthesis. J. Virol. 62:4016-4021.
27. Molla, A., A. V. Paul, and E. Wimmer. 1991. Cell-free de novo synthesis of poliovirus. Science 254:1647-1651.
28. Molla, A., A. V. Paul, and E. Wimmer. 1993. Effects of temperature and lipophilic agents on poliovirus formation and RNA synthesis in a cell-free system. J. Virol. 67:5932-5938.
29. pol. J. Virol. 68:5811-5818.
30. Neufeld, K. L., O. C. Richards, and E. Ehrenfeld. 1991. Purification, characterization, and comparison of poliovirus RNA polymerase from native and recombinant sources. J. Biol. Chem. 266:24212-24219.
31. Novak, J. E., and K. Kirkegaard. 1991. Improved method for detecting poliovirus negative strands used to demonstrate specificity of positive-strand encapsidation and the ratio of positive to negative strands in infected cells. J. Virol. 65:3384-3387.
32. Novak, J. E., and K. Kirkegaard. 1994. Coupling between genome translation and replication in an RNA virus. Genes Dev. 8:1726-1737.
33. Pasamontes, L., D. Egger, and K. Bienz. 1986. Production of monoclonal and monospecific antibodies against non-capsid proteins of poliovirus. J. Gen. Virol. 67:2415-2422.
34. Paul, A. V., A. Molla, and E. Wimmer. 1994. Studies of a putative amphipathic helix in the N-terminus of poliovirus protein 2C. Virology 199:188-199.
35. Paul, A. V., J. H. vanBoom, D. Filippov, and E. Wimmer. 1998. Protein-primed RNA synthesis by purified poliovirus RNA polymerase. Nature 393: 280-284.
36. Restrepo-Hartwig, M. A., and P. Ahlquist. 1996. Brome mosaic virus helicase- and polymerase-like proteins colocalize on the endoplasmic reticulum at sites of viral RNA synthesis. J. Virol. 70:8908-8916.
37. Sandoval, I. V., and L. Carrasco. 1997. Poliovirus infection and expression of the poliovirus protein 2B provoke the disassembly of the Golgi complex, the organelle target for the antipoliovirus drug Ro-090179. J. Virol. 71:4679-4693.
38. Schaad, M. C., P. E. Jensen, and J. C. Carrington. 1997. Formation of plant RNA virus replication complexes on membranes: role of an endoplasmic reticulum-targeted viral protein. EMBO J. 16:4049-4059.
39. Schlegel, A., T. H. Giddings, Jr., M. S. Ladinsky, and K. Kirkegaard. 1996. Cellular origin and ultrastructure of membranes induced during poliovirus infection. J. Virol. 70:6576-6588.
40. Sitzmann, J. H., and P. K. LeMotte. 1993. Rapid and efficient generation of PCR-derived riboprobe templates for in situ hybridization histochemistry. J. Histochem. Cytochem. 41:773-776.
41. Takegami, T., B. L. Semler, C. W. Anderson, and E. Wimmer. 1983. Membrane fractions active in poliovirus RNA replication contain VPg precursor polypeptides. Virology 128:33-47.
42. Teterina, N. L., K. M. Kean, A. E. Gorbalenya, V. I. Agol, and M. Girard. 1992. Analysis of the functional significance of amino acid residues in the putative NTP-binding pattern of the poliovirus 2C protein. J. Gen. Virol. 73:1977-1986.
43. Todd, S., J. S. Towner, D. M. Brown, and B. L. Semler. 1997. Replication-competent picornaviruses with complete genomic RNA 3′ noncoding region deletions. J. Virol. 71:8868-8874.
44. Troxler, M., D. Egger, T. Pfister, and K. Bienz. 1992. Intracellular localization of poliovirus RNA by in situ hybridization at the ultrastructural level using single-stranded riboprobes. Virology 191:687-697.
45. Van Dyke, T. A., and J. B. Flanegan. 1980. Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase. J. Virol. 35:732-740.
46. van Kuppeveld, F. J. M., J. M. Galama, J. Zoll, P. J. van den Hurk, and W. J. Melchers. 1996. Coxsackie B3 virus protein 2B contains cationic amphipathic helix that is required for viral RNA replication. J. Virol. 70:3876-3886.
47. van Kuppeveld, F. J. M., J. M. D. Galama, J. Zoll, and W. J. G. Melchers. 1995. Genetic analysis of a hydrophobic domain of coxsackie B3 virus protein 2B: a moderate degree of hydrophobicity is required for a cis-acting function in viral RNA synthesis. J. Virol. 69:7782-7790.
48. van Kuppeveld, F. J. M., P. J. van den Hurk, W. van der Vliet, J. M. Galama, and W. J. Melchers. 1997. Chimeric coxsackie B3 virus genomes that express hybrid coxsackievirus-poliovirus 2B proteins: functional dissection of structural domains involved in RNA replication. J. Gen. Virol. 78:1833-1840.
49. Young, D. C., D. M. Tuschall, and J. B. Flanegan. 1985. Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro. J. Virol. 54:256-264.
50. Young, I. D., R. J. Stewart, L. Ailles, A. Mackie, and J. Gore. 1993. Synthesis of digoxigenin-labeled cRNA probes for nonisotopic in situ hybridization using reverse transcription polymerase chain reaction. Biotech. Histochem. 68:153-158.