Microsomal acetaldehyde oxidation is negligible in the presence of ethanol

Alcoholism: Clinical and Experimental Research

Volumn 22, Issue 5, 1998, Pages 1165-1169

  Wu, Yue Sheng ^a,b   Salmela, Katja S ^a,b   Lieber, Charles S ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

Acetaldehyde Oxidation; CYP2E1; Ethanol; Microsomal Ethanol Oxidizing System (MEOS)

Indexed keywords

ACETALDEHYDE;

ALCOHOL CONSUMPTION; ALCOHOL METABOLISM; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; ENZYME SPECIFICITY; ENZYME SYNTHESIS; LIPID OXIDATION; LIVER MICROSOME; METABOLIC RATE; MICROSOME MEMBRANE; NONHUMAN; PRIORITY JOURNAL; RAT;

ANIMALIA;

EID: 0031683885     PISSN: 01456008     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1530-0277.1998.tb03717.x     Document Type: Article

References (20)

1. Lieber CS, DeCarli LM: Ethanol oxidation by hepatic microsomes: Adaptive increase after ethanol feeding. Science 162:917-918, 1968
2. Lieber CS, DeCarli LM: Hepatic microsomal ethanol oxidizing system: In vitro characteristics and adaptive properties in vivo. J Biol Chem 245:2505-2512, 1970
3. Lieber CS: Cytochrome P4502E1: Its physiological and pathological role. Physiol Rev 77:517-544, 1997
4. Lieber CS: Medical and Nutritional Complications of Alcoholism: Mechanisms and Management. Chap 2. New York, Plenum Press, 1992, pp 37-53
5. Lieber CS: Ethanol metabolism, cirrhosis and alcoholism. Clin Chim Acta 257:59-84, 1997
6. Koivula T, Koivusalo M: Different forms of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochim Biophys Acta 397:9-23, 1975
7. Tottmar SOC, Peterson H, Kiessling KH: The subcellular distribution and properties of aldehyde dehydrogenase in rat liver. Biochem J 135:577-586, 1973
8. Tsutsumi M, Takada A, Takase S: The intralobular distribution of aldehyde dehydrogenase isoenzymes: Differences with respect to species. Alcohol 5:33-38, 1988
9. Weiner H: Subcellular localization of acetaldehyde oxidation in liver. Ann N Y Acad Sci 492:25-34, 1987
10. Terelius Y, Norsten-Höög C, Cronholm T, Ingelman-Sundberg M: Acetaldehyde as a substrate for ethanol-inducible cytochrome P450 (CYP2E1). Biochem Biophys Res Commun 79:689-694, 1991
11. Kunitoh S, Asai H, Imaoka S, Funae Y, Monna T: Metabolism of acetaldehyde to acetate by rat hepatic P-450s: Presence of different metabolic pathway from acetaldehyde dehydrogenase system. Alcohol Clin Exp Res 20:22A-24A, 1996
12. Kunitoh S, Imaoka S, Hiroi T, Yabusaki Y, Monna T, Funae Y: Acetaldehyde as well as ethanol is metabolized by human CYP2E1. J Pharmacol Exp Ther 280:527-532, 1997
13. Lieber CS, DeCarli LM: Animal models of chronic ethanol toxicity. Methods Enzymol 233:585-594, 1994
14. Ardies CM, Lasker JM, Bloswick BP, Lieber CS: Purification of NADPH: Cytochrome C (cytochrome P-450) reductase from hamster liver microsomes by detergent extraction and affinity chromatography. Anal Biochem 162:39-46, 1987
15. Ohnishi K, Lieber CS: Reconstitution of the microsomal ethanol-oxidizing system: Qualitative and quantitative changes of cytochrome P-450 after chronic ethanol consumption. J Biol Chem 252:7124-7131, 1977
16. Tassaneeyakul W, Veronese ME, Birkett DJ, Gonzalez FJ, Miners JO: Validation of p-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA expression and microsomal kinetic techniques. Biochem Pharmacol 46:1975-1981, 1993
17. Greenfield NJ, Pietruszko R: Two aldehyde dehydrogenase from human liver: Isolation via affinity chromatography and characterization of the isozymes. Biochim Biophys Acta 483:35-45, 1977
18. Singh S, Fritze G, Fang B, Harada S, Paik YK, Eckey R, Agarwal DP: Inheritance of mitochondrial aldehyde dehydrogenase: Genotyping in Chinese, Japanese and South Korean families reveals dominance of the mutant allele. Human Genet 83:119-121, 1989
19. Enomoto N, Takase S, Takada N, Takada A: Alcoholic liver disease in heterozygotes of mutant and normal aldehyde dehydrogenase-2 genes. Hepatology 13:1071-1075, 1991
20. D(V/K) isotope effect of the cytochrome P-450-mediated oxidation of ethanol and its biological applications. Eur J Biochem 125:593-603, 1982