Application of a multi-endpoint cytotoxicity assay to the trypanocidal compounds 2-propen-1-amine derivatives and determination of their acute toxicity

In Vitro and Molecular Toxicology: Journal of Basic and Applied Research

Volumn 11, Issue 2, 1998, Pages 153-160

  De Conti, R ^a   Oliveira, D A ^a   Fernandes, A M A P ^a   Melo, P S ^a   Rodriguez, J A ^a   Haun, M ^a   De Castro, S L ^b   Souza Brito, A R M ^a   Duran, N ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ALLYLAMINE; ANTITRYPANOSOMAL AGENT; NEUTRAL RED; NIFURTIMOX; PROTOZOAL DNA;

ANIMAL CELL; ARTICLE; CELL STRAIN V 79; CONTROLLED STUDY; DNA CONTENT; DRUG CYTOTOXICITY; FIBROBLAST CULTURE; NONHUMAN; TOXICITY TESTING;

EID: 0031680591     PISSN: 10979336     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. BABICH, H., and BORENFREUND, E. (1990). Application of the neutral red cytotoxicity assay to in vitro toxicology. ATLA 18, 129-144.
2. BABICH, H., and BORENFREUND, E. (1991). Cytotoxicity and genotoxicity assays with cultured fish cells: A review. Toxicol. In Vitro. 5, 91-100.
3. BORENFREUND, E., and PUERNER, J.A. (1984). A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/RN-90). J. Tissue Culture Methods. 9, 7-9.
4. CINGI, M.R., DE ANGELIS, I., FORTUNATI, E., REGGIONI, D., BIANCHI, V., TIOZZO, R., and ZUCCO, F. (1991). Choice and standarization of test protocols in cytotoxicology: A multicenter approach. Toxicol. In Vitro. 5, 119-125.
5. CLEMEDSON, C., MCFARLANE-ABDULLA, E., ANDERSSON, M., et al. (1996). MEIC evaluation of acute systemic toxicity, Part I. Methodology of 68 in vitro toxicity assays used to test the first 30 reference chemicals. ATLA 24, 251-272.
6. COMBRIER, E., MÉTÉZEAU, P., RONOT, X., KIEFER-GACHELIN, H., and ADOLPHE, M. (1990). Comparative assessment of in vitro toxicity of xenobiotics using flow cytometry and spectrophotometry. Toxicol. In Vitro. 4, 751-755.
7. DE CASTRO, S.L. (1993). The challenge of Chagas's disease chemotherapy: An update of drugs assayed against Trypanosoma cruzi. Acta Tropica 53, 83-98.
8. DE CASTRO, S.L., and MEIRELLES, M.N.L. (1987). Effect of drugs on Trypanosoma cruzi and its interaction with heart muscle cells in vitro. Mem. Inst. Oswaldo Cruz 82, 209-215.
9. DE CONTI, R., GIMENEZ, S.M.N., HAUN, M., PILLI, R.A. DE CASTRO, S.L., and DURÁN, N. (1996a). Synthesis and biological activities of N,N-dimethyl-2-propen-1-amine derivatives. Eur. J. Med. Chem. 31, 915-918.
10. DE CONTI, R., SANTA RITA, R.M., DE SOUZA, E.M., MELO, P.S., HAUN, M., DE CASTRO, S.L., and DURAŃ N. (1996b). In vitro trypanocidal activities of a novel series of N,N-dimethyl-2-propen-1-amine derivatives. Microbios 85, 83-87.
11. DENIZOT, F., and LANG, R. (1986). Rapid colorimetric assay for cell growth and survival. Modifications to the tratazolium dye procedure giving imporved sensitivity and reliability. J. Immunol. Methods. 89, 271-277.
12. DOYLE, P.S., and WEINBACH, E.C. (1989). The activity of tricyclic antidepressant drug against Trypamonosoma cruzi. Exper. Parasitol. 68, 230-234.
13. DURÁN, N., and HAUN, M. (1991). Trypanocides: State of art. Mem. Inst. Oswaldo Cruz 86, 29-30.
14. FORSBY, A., ANDERSSON, M., LEWAN, L., and STERNER, O. (1991). The cytotoxicity of 22 sesquiterpenoids unsaturated dialdehydes, as determined the neutral red absorption and by protein determination. Toxicol. in Vitro. 5, 9-14.
15. FRY, J.R., HAMMOND, A.H., ATMACA, M., DHANJAL, P., and WILKINSON, D.J. (1995). Toxicity testing with hepatocytes: Some methodological aspects. ATLA 23, 91-96.
16. GORLA, N.B., LEDESMA, O.S., BARBIERI, G.P., and LARRIPA, I.B. (1989). Thirtheenfold increase of chromosomal aberrations non-randomly distributed in chagasic children with nifurtimox. Mutation Res. 224, 263-267.
17. HAMMOND, D.J., COVER, B., and GUTTERIDGE, W.E. (1984). A novel series of chemical structures active in vitro against the trypamastigote form of Trypanosoma cruzi. Trans. Roy. Soc. Trop. Med. Hyg. 78, 91-95.
18. HAUN, M., PEREIRA, M.F., HOFMANN, M.E., JOYAS, A., CAMPOS, V., FILARDY, L.D., DE CASTRO, S.L., and DURÁN, N. (1992). Bacterial chemistry. VI. Biological activities and cytotoxicity of 1,3-dihydro-2H-indol-2-one derivatives. Biol. Res. 25, 21-25.
19. 2+ ions of Escherichia coli. Chem. Spect. Bioavailab. 5, 97-100.
20. KAMINSKY, R., SCHMID, C., and BRUN, R. (1996). An "In vitro selectivity index" for evaluation of cytotoxicity of antitrypanosomal compounds. In Vitro Toxicol. 9, 315-324.
21. LOVELAND, B.E., JOHNS, T.G., MACKAY, I.R., VAILLANT, F., WANG, Z.-X., and HERTZOG, P.J. (1992). Validation of the MTT dye assay for enumeration of cells in proliferative and antiproliferative assays. Biochem. Intern. 27, 501-510.
22. LULLMANN, H., LULLMANN-RAUCH, R., and WASSERMANN, O. (1978). Lipidosis induced by amphiphilic cationic drugs. Biochem. Pharmacol. 27, 1103-1108.
23. MELO, P.S., and HAUN, M. (1998). Application of multi-endpoints cytotoxicity assay system to two trypanosomicides: Nifurtimox and the bacterial metabolites prodigiosin. Toxicol. in Vitro. submitted.
24. MIYAJIMA, A., KAWANISHI, T., GUO, X., SUNOUCHI, M., TANAKA, A., and OHNO, Y. (1996). Metabolism of N-alkyl and cyclic nitroamines in freshly isolated rat hepatocytes. In Vitro Toxicol. 9, 149-155.
25. MITCHELL, R.H., ALLAN, D., BOWLEY, M., and BRINDLEY, D.N. (1976). A possible metabolic explanation for drug-induced phospholipidosis. J. Pharm. Pharmacol. 28, 331-332.
26. OLIVIER, P., TESTARD, P., MARZIN, D., and ABBOTT, D. (1995). Effect of high polyol concentration on the neutral red absorption assay and tetrazolium-MTT test of rat hepatocytes in primary culture. Toxicol. in Vitro. 9, 133-138.
27. REPETTO, G., and SANZ, P. (1993). Neutral red uptake, cellular growth and lysosomal function: In vitro effect of 24 metals. ATLA 21, 501-507.
28. RIDDELL, R.J., CLOTHIER, R.H., and BALLS, M. (1986). An evaluation of three in vitro cytotoxicity assays. Food Chem. Toxicol. 24, 469-471.
29. RENZI, D., VALTOLINI, M., and FORSTER, R. (1993). The evaluation of a multi-endpoints cytotoxicity assay system. ATLA. 21, 89-96.
30. ROGUET, R., COTOVIO, J., GAETANI, Q., DOSSOU, K.G., and ROUGIER, A. (1993). Cytotoxicity of 28 MEIC chemicals to rat hepatocytes using two viability endpoints: Correlation with acute toxicity data in rat and man. ATLA. 21, 216-224.
31. SCUDIERO, D.A., SHOEMAKER, PH., PAULL, K.D., MONKS, A., TIERNEY, S., NOFZIGER, T.H., CURRENS, M.J., SENIFF, D., and BOYD, M.R. (1988). Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Res. 48, 4827-4833.
32. SILVA, L.H.P., and NUSSENZWEIG, V. (1953). Sobre uma cepa de trypanosoma cruzi altamente virulenta para camundong branco. Folia Clin. Biol. 20, 191-207.
33. SLATER, T.F., SAWYER, B., and STRAULI, U. (1963). Studies on succinate-tetrazolium reductase system III. Points of coupling of four different tetrazolium salts. Biochim. Biophys. Acta 77, 383-393.
34. SOUZA-BRITO, A.R.M., RODRIGUEZ, J.A., HIRUMALIMA, C.A., HAUN, M., and NUNES, D.S. (1998). Antiulcerogenic activity of trans-dehydrocrotonin from Corton cajucara Benth. Planta Med. 64, 126-129.
35. TEIXEIRA, A.R.L., CALIXTO, M.A., and TEIXEIRA, M.L. (1994). Chagas's disease carcinogenic activity of the antitrypanosomal nitroarenes in mice. Mutat. Res. 305, 189-196.