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85030346759
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23 The success of this approach would depend on the number of endogenous cysteines that might also react with NEM to increase the background and on the ease and efficiency of purification.
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85030341682
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note
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These findings, however, do not rule out the possibility that some of the effects of modification are on another protein that directly interacts with the transporter and is necessary for both transport and binding.
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39
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85030341367
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note
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34
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41
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85030348914
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note
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If a water-accessible cysteine sulfhydryl is critical to the expression or function of the transporter and if reaction of such a cysteine with the MTS reagents affects binding or transport, then it may be impossible to produce the appropriate MTS-insensitive mutant. Thus, it is possible that a given protein will not be amenable to study with this method, and unfortunately this cannot be predicted in advance.
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85030343039
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note
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15-18 MTSEA is slightly more reactive than is MTSET, with accessible cysteines in the dopamine D2 receptor; however, MTSEA is smaller than MTSET (and the negatively charged MTSES), and it might gain more rapid access to certain residues in the water-accessible binding-site crevice simple because of reduced steric hindrance. Moreover, MTSEA shares with dopamine the ethylamine moiety, and its increased reactivity relative to MTSET may also result from an affinity reaction with the dopamine binding site.
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