메뉴 건너뛰기




Volumn 296, Issue , 1998, Pages 331-346

Probing structure of neurotransmitter transporters by substituted- cysteine accessibility method

(1)  Javitch, Jonathan A a  

a NONE

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; CYSTEINE; DOPAMINE; DOPAMINE 2 RECEPTOR; NEUROTRANSMITTER;

EID: 0031660683     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(98)96025-6     Document Type: Article
Times cited : (48)

References (53)
  • 24
    • 85030346759 scopus 로고    scopus 로고
    • note
    • 23 The success of this approach would depend on the number of endogenous cysteines that might also react with NEM to increase the background and on the ease and efficiency of purification.
  • 38
    • 85030341682 scopus 로고    scopus 로고
    • note
    • These findings, however, do not rule out the possibility that some of the effects of modification are on another protein that directly interacts with the transporter and is necessary for both transport and binding.
  • 39
    • 85030341367 scopus 로고    scopus 로고
    • note
    • 34
  • 41
    • 85030348914 scopus 로고    scopus 로고
    • note
    • If a water-accessible cysteine sulfhydryl is critical to the expression or function of the transporter and if reaction of such a cysteine with the MTS reagents affects binding or transport, then it may be impossible to produce the appropriate MTS-insensitive mutant. Thus, it is possible that a given protein will not be amenable to study with this method, and unfortunately this cannot be predicted in advance.
  • 51
    • 85030343039 scopus 로고    scopus 로고
    • note
    • 15-18 MTSEA is slightly more reactive than is MTSET, with accessible cysteines in the dopamine D2 receptor; however, MTSEA is smaller than MTSET (and the negatively charged MTSES), and it might gain more rapid access to certain residues in the water-accessible binding-site crevice simple because of reduced steric hindrance. Moreover, MTSEA shares with dopamine the ethylamine moiety, and its increased reactivity relative to MTSET may also result from an affinity reaction with the dopamine binding site.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.