Preparation of mycobacterial DNA from blood culture fluids by simple alkali wash and heat lysis method for PCR detection

Journal of Clinical Microbiology

Volumn 34, Issue 8, 1996, Pages 1985-1991

  Kulski, Jerzy K ^a   Pryce, Todd ^a  

^a ROYAL PERTH HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

2 PROPANOL; BACTERIAL DNA; SODIUM IODIDE;

ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; DIAGNOSTIC ACCURACY; MYCOBACTERIOSIS; MYCOBACTERIUM INTRACELLULARE AVIUM; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; MYCOBACTERIUM; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM-INTRACELLULARE; MYCOBACTERIUM INTRACELLULARE; MYCOBACTERIUM TUBERCULOSIS;

EID: 0029891885     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.8.1985-1991.1996     Document Type: Article

References (36)

1. Benson, C. A. 1994. Mycobacterium tuberculosis and Mycobacterium avium complex disease in patients with HIV infection. Curr. Opin. Infect. Dis. 7:95-107.
2. Bianchi, N., C. Mischiati, G. Feriotto, D. Fiorentino, S. Di Biase, N. Apicella, and R. Gambari. 1994. Capillary electrophoresis: detection of hybridization between synthetic oligonucleotides and HIV-1 genomic DNA amplified by polymerase-chain reaction. J. Virol. Methods 47:321-329.
3. Bottger, E. C., A. Teske, P. Kirschner, S. Bost, H. R. Chang, V. Beer, and B. Hirschel. 1992. Disseminated Mycobacterium genavense infection in patients with AIDS. Lancet 340:76-80.
4. Buck, G. H., L. C. O'Hara, and J. T. Summersgill. 1992. Rapid, simple method for treating clinical specimens containing Mycobacterium tuberculosis to remove DNA for polymerase chain reaction. J. Clin. Microbiol. 30:1331-1334.
5. Cormican, M. G., T. Barry, F. Gannon, and J. Flynn. 1992. Use of polymerase chain reaction for early identification of Mycobacterium tuberculosis in positive cultures. J. Clin. Pathol. 45:601-604.
6. Ellner, J. J., M. J. Goldberger, and D. M. Parenti. 1991. Mycobacterium avium infection and AIDS: a therapeutic dilemma in rapid evolution. J. Infect. Dis. 163:1326-1335.
7. Ephraim, D. A., and E. D. Spitzer. 1994. Use of acridinium-ester-labeled DNA probes for identification of mycobacteria in BACTEC 13A blood cultures. Diagn. Microbiol. Infect. Dis. 18:137-139.
8. Evans, K. D., A. S. Nakasone, P. A. Sutherland, L. M. de la Maza, and E. M. Peterson. 1992. Identification of Mycobacterium tuberculosis and Mycobacterium avium-M. intracellulare directly from primary BACTEC cultures by using acridinium-ester-labeled DNA probes. J. Clin. Microbiol. 30:2427-2431.
9. Forbes, B. A., and K. E. S. Hicks. 1993. Direct detection of Mycobacterium tuberculosis in respiratory specimens in a clinical laboratory by polymerase chain reaction. J. Clin. Microbiol. 31:1688-1694.
10. Fries, J. W. U., R. J. Patel, W. F. Piessens, and D. F. Wirth. 1990. Genus- and species-specific DNA probes to identify mycobacteria using the polymerase chain reaction. Mol. Cell. Probes 4:87-105.
11. Fries, J. W. U., R. J. Patel, W. F. Piessens, and D. F. Wirth. 1991. Detection of untreated mycobacteria by using polymerase chain reaction and specific DNA probes. J. Clin. Microbiol. 29:1744-1747.
12. Havlir, D., C. A. Kemper, and S. C. Deresinski. 1993. Reproducibility of lysis-centrifugation cultures for quantification of Mycobacterium avium complex bacteremia. J. Clin. Microbiol. 31:1794-1798.
13. Hughes, M. S., R. A. Skuce, L.-A. Beck, and S. D. Neill. 1993. Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16S rRNA gene sequences. J. Clin. Microbiol. 31:3216-3222.
14. Kent, P. T., and G. P. Kubica. 1985. Public health mycobacteriology. A guide for the level III laboratory. Centers for Disease Control, Atlanta.
15. Kocagoz, T., E. Yilmaz, S. Ozkara, S. Kocagoz, M. Hayran, M. Sachedeva, and H. F. Chambers. 1993. Detection of Mycobacterium tuberculosis in sputum samples by polymerase chain reaction using a simplified procedure. J. Clin. Microbiol. 31:1435-1438.
16. Kubica, G. P., W. E. Dye, M. L. Cohn, and G. Middlebrook. 1963. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am. Rev. Respir. Dis. 87:775-779.
17. Kulski, J. K. 1994. Quantitation of human cytomegalovirus DNA in leukocytes by end-point titration and duplex polymerase chain reaction. J. Virol. Methods 49:195-208.
18. Kulski, J. K., C. Khinsoe, T. Pryce, and K. Christiansen. 1995. Use of a multiplex PCR to detect and identify Mycobacterium avium and M. intracellulare in blood culture fluids of AIDS patients. J. Clin. Microbiol. 33:668-674.
19. Kulski, J. K., and T. Pryce. Unpublished data.
20. Liedtke, W., B. Opalka, C. W. Zimmermann, and E. Schmid. 1994. Different methods of sample preparation influence sensitivity of Mycobacterium tuberculosis and Borrelia burgdorferi PCR. PCR Methods Appl. 3:301-304.
21. Masur, H. 1993. Special report. Recommendations on prophylaxis and therapy for disseminated Mycobacterium avium complex disease in patients infected with human immunodeficiency virus. N. Engl. J. Med. 329:898-905.
22. McGinnis, M. R. 1980. Laboratory handbook of medical mycology, U.K. ed., p. 82. Academic Press, Inc., New York.
23. Musial, C. E., F. R. Cockerill III, and G. D. Roberts. 1988. Fungal infections of the immunocompromised host: clinical and laboratory aspects. Microbiol. Rev. 1:349-364.
24. Nightingale, S. D., L. T. Byrd, P. M. Southern, J. D. Jockusch, S. X. Cal, and B. A. Wynne. 1992. Incidence of Mycobacterium avium-intracellulare complex bacteremia in human immunodeficiency virus-positive patients. J. Infect. Dis. 165:1082-1085.
25. Nolte, F. S., B. Metchock, J. E. McGowan, Jr., A. Edwards, O. Okwumabua, C. Thurmond, P. S. Mitchell, B. Plikaylis, and T. Shinnick. 1993. Direct detection of Mycobacterium tuberculosis in sputum by polymerase chain reaction and DNA hybridization. J. Clin. Microbiol. 31:1777-1782.
26. Noordhoek, G. T., A. H. J. Kolk, G. Bjune, D. Catty, J. W. Dale, P. E. M. Fine, P. Godfrey-Faussett, S.-N. Cho, T. Shinnick, S. B. Svenson, S. Wilson, and J. D. A. Van Embden. 1994. Sensitivity and specificity of PCR for detection of Mycobacterium tuberculous: a blind comparison study among seven laboratories. J. Clin. Microbiol. 32:277-284.
27. Ratman, S., F. A. Stead, and M. Howes. 1987. Simplified acetylcysteine-alkali digestion-decontamination procedure for isolation of mycobactcria from clinical specimens. J. Clin. Microbiol. 25:1428-1432.
28. Small, P. M., R. W. Shafter, P. C. Hopewell, S. P. Singh, M. J. Murphy, E. Desmond, M. F. Sierra, and G. K. Schoolnik. 1993. Exogenous reinfection with multidrug-resistant Mycobacterium tuberculosis in patients with advanced HIV infection. N. Engl. J. Med. 328:1137-1144.
29. Sritharan, V., and R. H. Barker, Jr. 1991. A simple method for diagnosing M. tuberculosis infection in clinical samples using PCR. Mol. Cell. Probes 5:385-395.
30. Tsukamura, M., and S. Yamori. 1991. Differentiation between Mycobacterium avium and M. intracellulare by phenetic characteristics. Tubercle 72:308.
31. Vestal, A. L. 1975. Procedures for the isolation and identification of mycobacteria. Training and Consultative Division, Center for Disease Control, Atlanta.
32. Wald, A., M. B. Coyle, L. C. Carlson, R. L. Thompson, and T. M. Hooton. 1992. Infection with fastidious Mycobacterium resembling Mycohacterium simiae in seven patients with AIDS. Ann. Intern. Med. 117:586-589.
33. Whelen, A. C., T. A. Felmlee, J. M. Hunt, D. L. Williams, G. D. Roberts, L. Stockman, and D. H. Persing. 1995. Direct genotypic detection of Mycobacterium tuberculosis rifampin resistance in clinical specimens by using single-tube heminested PCR. J. Clin. Microbiol. 33:556-561.
34. Wilton, S., and D. Cousins. 1992. Detection and identification of multiple mycobacteria pathogens by DNA amplification in a single tube. PCR Methods Appl. 1:269-27.1.
35. Yamaguchi, R., K. Matsuo, A. Yamazaki, M. Takahashi, Y. Fukasawa, M. Wada, and C. Abe. 1992. Cloning and expression of gene for the Avi-3 antigen of Mycobacterium avium and mapping of its epitopes. Infect. Immun. 60:1210-1216.
36. Yamori, S., and M. Tsukamura. 1992. Comparison of prognosis of pulmonary diseases caused by Mycobacterium avium and by Mycobacterium intracellulare. Chest 102:89-90.