Late G1 accumulation after 2 Gy of γ-irradiation is related to endogenous Raf-1 protein expression and intrinsic radiosensitivity in human cells

British Journal of Cancer

Volumn 77, Issue 8, 1998, Pages 1220-1228

  Warenius, H M ^a   Jones, M ^a   Jones, M D ^a   Browning, P G ^a   Seabra, L A ^a   Thompson, C C M ^a  

^a UNIVERSITY OF LIVERPOOL   (United Kingdom)

Author keywords

Cell cycle checkpoint; G1 delay; Human cell line; Irradiation; Oncogene; Raf 1

Indexed keywords

RAF PROTEIN;

ARTICLE; CELL CYCLE; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CONTROLLED STUDY; FLOW CYTOMETRY; GAMMA IRRADIATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTO ONCOGENE; RADIOSENSITIVITY; CELL COUNT; CELL CULTURE; GAMMA RADIATION; METABOLISM; RADIATION DOSE; RADIATION EXPOSURE; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL COUNT; FLOW CYTOMETRY; G1 PHASE; GAMMA RAYS; HUMANS; PROTO-ONCOGENE PROTEINS C-RAF; RADIATION TOLERANCE; S PHASE; TUMOR CELLS, CULTURED;

EID: 0031596562     PISSN: 00070920     EISSN: None     Source Type: Journal    
DOI: 10.1038/bjc.1998.206     Document Type: Article

References (45)

1. Cipl/Wafl proteins following γ-irradiation of human lymphoma cells. Cancer Res 55: 2387-2393
2. Barnard D, Diaz B, Hettich L, Chuang E, Zhang X, Avruch J and Marshall M (1995) Identification of the sites of interaction between c-Raf-1 and Ras-GTP. Oncogene 10: 1283-1290
3. Bernhard EJ, Muschel RJ, Bakanauskas VJ and McKenna WG (1996) Reducing the radiation-induced G2 delay causes HeLa cells to undergo apoptosis instead of mitotic death. Int J Radiat Biol 69: 575-584
4. Browning PG (1997) Photo-oncogene expression and intrinsic cellular radiosensitivity. PhD thesis. University of Liverpool Faculty of Medicine.
5. Burnet, NG, Nyman J, Turesson I, Wurm R, Yarnold JR and Peacock JH (1992) Prediction of normal tissue tolerance to radiotherapy from in vitro cellular radiation sensitivity. Lancet 339: 1570-1571
6. Busse PM and Bose SK (1978) The action of caffeine on X-irrudtated HeLa cells. III. Enhancement of X-ray induced killing during G, arrest. Radiat Res 76: 292-307
7. Cheong N, Wang Y, Jackson M and Iliakis G (1992) Radiation-sensitive irs mutants rejoin DNA double-strand breaks with efficiency similar to that of parental V79 cells but show altered response to radiation-induced G2 delay. Mutat Res 274: 111-122
8. Deacon J, Peckham MJ and Steel GG (1984) The radioresponsiveness of human tumours and the initial slope of the cell-survival curve. Radiother Oncol 2: 317-323
9. Fabian JR, Daar IO and Morrison DK (1993) Critical tyrosine residues regulate the enzymatic and biological activity of Raf-1 kinase. Mol Cell Biol 13: 7170-7179
10. Fan S, El-Deiry WS, Bae I, Freeman J, Jondle D, Bhatia K, Fornace AJ Jr, Magrath I, Kohn, KW and O'Connor, PM (1994) p53 gene mutations are associated with decreased sensitivity of human lymphoma cells to DNA damaging agents. Cancer Res 54: 5824-5830
11. Fertil B and Malaise EP (1981) Inherent cellular radiosensitivity as a basic concept for human tumor radiotherapy. Int J Radiat Oncol Biol Phys 7: 621-629
12. Guillouf C, Rosselli F, Krishnaraju K, Moustacchi E, Hoffman B and Liebermann DA (1995) p53 involvement in control of G2 exit of the cell cycle: role in DNA damage-induced apoptosis. Oncogene 10: 2263-2270
13. Iliakis G, Metzger L, Muschel RJ and McKenna WG (1990) Induction and repair of double strand breaks in radiation-resistant cells obtained by transformation of primary rat embryo cells with the oncogenes H-ras and v-myc. Cancer Res 50: 6575-6579
14. Jung M and Dritschilo A (1994) Modification of the radiosensitivity of human testicular cancer cells by simian virus 40 sequences. Radiat Res 133: 73-79
15. Kasid U, Suy S, Dent P, Ray S. Whiteside TL and Sturgill TW (1996) Activation of Raf by ionizing radiation. Nature 382: 813-816
16. Kastan MB, Onyekwere O, Sidransky D, Vogelstein B and Craig RW (1991) Participation of p53 in the cellular response to DNA damage. Cancer Res 51: 6304-6311
17. Kawashima K, Mihara K, Usuki H, Shimizu N and Namba M (1995) Transfected mutant p53 gene increases x-ray induced cell killing and mutation in human fibroblasts immortalised with 4-Nitroquinoline 1-oxide but does not induce neoplastic transformation of cells. Int J Cancer 61: 76-79
18. Kelland LR, Edwards SM and Steel GG (1988) Induction and rejoining of DNA double-strand breaks in human cervix carcinoma cell lines of differing radiosensitivity. Rad Res 116: 526-538
19. Kim SH, Khil MS. Ryu S and Kim JH (1992) Enhancement of radiation response on human carcinoma cells in culture by pentoxifyllene. Int J Radiat Oncol Biol Phys 25: 61-65
20. Lavoie JN, L'Allemain G, Brunet A, Muller R and Pouysegur J (1996) Cyclin D1 expression is regulated positively by the p42/44MAPK and negatively by the p38/HOGMAPK pathway. J Biol Chem 271: 20608-20616
21. Lee JM and Bernstein A (1993) p53 mutations increase resistance to ionizing radiation. Proc Natl Acad Sci USA 90: 5742-5746
22. 2 checkpoint response to ionizing radiation. Cancer Res 55: 2500-2502
23. Lotem J and Sachs L (1993) Hematopoietic cells from mice deficient in wild-type p53 are more resistant to induction of apoptosis by some agents. Blood 82: 1092-1096
24. Lowe SW, Bodis S, McClatchey A, Remington L, Ruley HE, Fisher DE, Houseman DE and Jacks T (1994) p53 status and the efficacy of cancer therapy in vivo. Science 266: 807-810
25. McIlwrath AJ, Vasey PA, Ross GM and Brown R (1994) Cell cycle arrests and radiosensitivity of human tumor cell lines: dependence on wild-type p53 for radiosensitivity. Cancer Res 54: 3718-3722
26. 2 delay in radiation-resistant cells obtained by transformation of primary rat embryo cells with the oncogenes H-ras and v-myc. Radiat Res 125: 283-287
27. Maity A, McKenna WG and Muschel RJ (1994) The molecular basis for cell cycledelays following ionizing radiation. Radiother Oncol 31: 1-13
28. Meyn MS (1995) Ataxia telangiectasia and cellular responses to DNA damage. Cancer Res 55: 5991
29. Nunez MI, Villalobos M, Olea N, Valenzuela MT, Pedraza V, McMillan TJ and Ruiz de Almodovar JM (1995) Radiation-induced DNA double-strand break rejoining in human tumour cells. Br J Cancer 71: 311-316
30. Powell SN and McMillan TJ (1994) The repair fidelity of restriction enzyme-induced double strand breaks in plasmid DNA correlates with radioresistance in human tumor cell lines. Int J Rad Oncol Biol Phys 29: 1035-1040
31. Scheistl RH, Reynolds P, Prakash S and Prakash L (1989) Cloning and sequence analysis of the Saccharomyces cerevisiae RAD9 gene and further evidence that its product is required for cell cycle arrest induced by DNA damage. Mol Cell Biol 9: 1882-1896
32. Schwartz JL, Mustafi R, Beckett MA, Gzyzewski EA, Farhangi E, Grdina DJ, Rotmensch J and Weichselbaum RR (1991) Radiation-induced DNA double-strand break frequencies in human squamous cell carcinoma cell lines of different radiation sensitivities. Int J Radiat Biol 59: 1314-1352
33. Shengfeng L, Janosch P, Tanji M, Rosenfeld GC, Waymire JC, Mischak H, Kolch W and Sedivy JM (1995) Regulation of Raf-1 kinase activity by the 14-3-3 family of proteins. EMBO J 14: 685-696
34. Siles E, Villalobos M, Valenzuela MT, Nunez MI, Gordon A, McMillan TJ, Pedraza V and Ruiz de Almodovar JM (1996) Relationship between p53 status and radiosensitivity in human tumour cell lines. Br J Cancer 73: 581-588
35. Stewart N, Hicks GG, Paraskevas F and Mowat M (1995) Evidence for a second cell cycle block at G2/M by p53. Oncogene 10: 109-115
36. Su LN and Little JB (1993) Prolonged cell cycle delay in radioresistant human cell lines transfected with activated ras oncogene and/or simian virus 40 T-antigen. Radiat Res 133: 73-79
37. Tobey RA (1975) Different drugs arrest cells at a number of distinct stages in G2. Nature 254: 245-247
38. Unger C, Kress S, Buchmann A and Schwarz M (1994) γ-irradiation-induced micronuclei from mouse hepatoma cells accumulate high levels of the tumour suppressor gene p53. Cancer Res 54: 3651-3655
39. Warenius HM, Britten RA and Peacock JH (1994a) The relative cellular radiosensitivity of 30 human in vitro cell lines of different histological type to high LET 62.5 MeV (p→Be:) fast neutrons and 4 MeV photons. Radiother Oncol 30: 83-89
40. Warenius HM, Browning PGW, Britten RA, Peacock JA and Rapp UR (1994b) C-raf-1 proto-oncogene expression relates to radiosensitivity rather than radioresistance. Eur J Cancer 30: 369-375
41. 2 phase after 2 Gy gamma irradiation is faster in radiosensitive human cells with high expression of the RAF-1 proto-oncogene. Radiat Res 146: 485-493
42. Warenius HM, Seabra LA and Maw P (1996b) Sensitivity to cis-diamminedichloroplatinum in human cancer cells is related to expression of cyclin DI but not c-Raf-1 protein. Int J Cancer 67: 224-231
43. Whitaker SJ, Ung YC and McMillan TJ (1995) DNA double strand break induction and rejoining as determinants of human tumour cell radiosensitivity. A pulsed-field gel electrophoresis study. Int J Radiat Biol 67: 7-18
44. Wurm R, Burnet NG, Duggal N, Yarnold JR and Peacock JH (1995) Cellular radiosensitivity and DNA damage in primary human fibroblasts. Int J Radiat Oncol Biol Phys 30: 625-633
45. Zhen W, Denault CM, Loviscek K, Walter S, Geng L and Vaughan ATM (1995) The relative radiosensitivity of TK6 and WI-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status. Mutat Res 346: 85-92