Use of a β1 integrin-deficient human T cell to identify β1 integrin cytoplasmic domain sequences critical for integrin function

Molecular Biology of the Cell

Volumn 9, Issue 10, 1998, Pages 2715-2727

  Romzek, Nadine C ^a   Harris, Estelle S ^c   Dell, Cheryl L ^b   Skronek, Jeffrey ^b   Hasse, Elizabeth ^d   Reynolds, Pamela J ^d   Hunt III, Stephen W ^b   Shimizu, Yoji ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b PFIZER INC   (United States)

^c UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; CELL ADHESION; CELL MEMBRANE; CONFORMATIONAL TRANSITION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; RECEPTOR UPREGULATION; SITE DIRECTED MUTAGENESIS; T LYMPHOCYTE ACTIVATION; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYSIOLOGY; POINT MUTATION; SEQUENCE ALIGNMENT; T LYMPHOCYTE;

BETA1 INTEGRIN; FIBRONECTIN; PRIMER DNA; RECOMBINANT PROTEIN; TYROSINE;

AMINO ACID SEQUENCE; ANTIGENS, CD29; BASE SEQUENCE; CELL ADHESION; DNA PRIMERS; FIBRONECTINS; HUMANS; JURKAT CELLS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; T-LYMPHOCYTES; TRANSFECTION; TYROSINE;

EID: 0031596163     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.9.10.2715     Document Type: Article

References (40)

1. Akiyama, S.K., Yamada, S.S., Yamada, K.M., and LaFlamme, S.E. (1994). Transmembrane signal transduction by integrin cytoplasmic domains expressed in single-subunit chimeras. J. Biol. Chem. 269, 15961-15964.
2. Arroyo, A.G., Sánchez-Mateos, P., Campanero, M.R., Martín-Padura, I., Dejana, E., and Sánchez-Madrid, F. (1992). Regulation of the VLA integrin-ligand interactions through the β1 subunit. J. Cell Biol. 117, 659-670.
3. Bazzoni, G., and Hemler, M.E. (1998). Are changes in integrin affinity and conformation overemphasized? Trends Biochem. Sci. 23, 30-34.
4. 1 integrin epitope induced by soluble ligand and manganese, but inhibited by calcium. J. Biol. Chem. 270, 25570-25577.
5. Campbell, J.J., Hedrick, J., Zlotnik, A., Siani, M.A., Thompson, D.A., and Butcher, E.C. (1998). Chemokines and the arrest of lymphocytes rolling under flow conditions. Science 279, 381-384.
6. Chen, Y.-P., O'Toole, T.E., Shipley, T., Forsyth, J., LaFlamme, S.E., Yamada, K.M., Shattil, S.J., and Ginsberg, M.H. (1994). "Inside-out" signal transduction inhibited by isolated integrin cytoplasmic domains. J. Biol. Chem. 269, 18307-18310.
7. Chesnut, J.D., Baytan, A.R., Russell, M., Chang, M.P., Bernard, A., Maxwell, I.H., and Hoeffler, J.P. (1996). Selective isolation of transiently transfected cells from a mammalian cell population with vectors expressing a membrane anchored single-chain antibody. J. Immunol. Methods 193, 17-27.
8. Diamond, M.S., and Springer, T.A. (1994). The dynamic regulation of integrin adhesiveness. Curr. Biol. 4, 506-517.
9. Dustin, M.L., and Springer, T.A. (1989). T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature 341, 619-624.
10. Filardo, E.J., Brooks, P.C., Deming, S.L., Damsky, C., and Cheresh, D.A. (1995). Requirement of the NPXY motif in the integrin β3 subunit cytoplasmic tail for melanoma cell migration in vitro and in vivo. J. Cell Biol. 130, 441-450.
11. Finkelstein, L.D., Reynolds, P.J., Hunt, S.W., III, and Shimizu, Y. (1997). Structural requirements for β1 integrin-mediated tyrosine phosphorylation in human T cells. J. Immunol. 159, 5355-5363.
12. Gibbs, R.A., Nguyen, P.N., McBride, L.J., Koepf, S.M., and Caskey, C.T. (1989). Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA. Proc. Natl. Acad. Sci. USA 86, 1919-1923.
13. Hibbs, M.L., Jakes, S., Stacker, S.A., Wallace, R.W., and Springer, T.A. (1991a). The cytoplasmic domain of the integrin lymphocyte function-associated antigen 1 β subunit: sites required for binding to intercellular adhesion molecule 1 and the phorbol ester-stimulated phosphorylation site. J. Exp. Med. 174, 1227-1238.
14. Hibbs, M.L., Xu, H., Stacker, S.A., and Springer, T.A. (1991b). Regulation of adhesion to ICAM-1 by the cytoplasmic domain of LFA-1 integrin β subunit. Science 251, 1611-1613.
15. Hughes, P.E., O'Toole, T.E., Ylänne, J., Shattil, S.J., and Ginsberg, M.H. (1995). The conserved membrane-proximal region of an integrin cytoplasmic domain specifies ligand binding affinity. J. Biol. Chem. 270, 12411-12417.
16. 90MEL-14) induced by activators of protein kinase C: receptor shedding due to accelerated proteolytic cleavage at the cell surface. J. Immunol. 144, 3130-3136.
17. Kishimoto, T.K., Jutila, M.A., Berg, E.L., and Butcher, E.C. (1989). Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science 245, 1238-1241.
18. 2 integrins. J. Clin. Invest. 100, 1725-1733.
19. LaFlamme, S.E., Thomas, L.A., Yamada, S.S., and Yamada, K.M. (1994). Single subunit chimeric integrins as mimics and inhibitors of endogenous integrin functions in receptor localization, cell spreading and migration, and matrix assembly. J. Cell Biol. 126, 1287-1298.
20. 3 by using cell-permeable peptide analogs. Proc. Natl. Acad. Sci. USA 93, 11819-11824.
21. Luque, A., Gómez, M., Puzon, W., Takada, Y., Sánchez-Madrid, F., and Cabañas, C. (1996). Activated conformations of very late activation integrins detected by a group of antibodies (HUTS) specific for a novel regulatory region (355-425) of the common β1 chain. J. Biol. Chem. 271, 11067-11075.
22. Mobley, J.L., Ennis, E., and Shimizu, Y. (1994). Differential activation-dependent regulation of integrin function in cultured human T-leukemic cell lines. Blood 83, 1039-1050.
23. Mobley, J.L., Ennis, E., and Shimizu, Y. (1996). Isolation and characterization of cell lines with genetically distinct mutations downstream of protein kinase C that result in defective activation-dependent regulation of T cell integrin function. J. Immunol. 156, 948-956.
24. Mobley, J.L., Romzek, N.C., and Shimizu, Y. (1995). Integrin activation in lymphocyte adhesion. In: Handbook of Experimental Immunology, vol. 68, ed. D.M. Weir, L.A. Herzenberg, C. Blackwell, and Le. A. Herzenberg, Cambridge, MA: Blackwell Scientific, 1-68.11
25. O'Toole, T.E., Ylanne, J., and Culley, B.M. (1995). Regulation of integrin affinity states through an NPXY motif in the β subunit cytoplasmic domain. J. Biol. Chem. 270, 8553-8558.
26. Postigo, A.A., Sánchez-Mateos, P., Lazarovits, A.I., Sánchez-Madrid, F., and De Landázuri, M.O. (1993). α4β7 integrin mediates B cell binding to fibronectin and vascular cell adhesion molecule-1: expression and function of α4 integrins on human B lymphocytes. J. Immunol. 151, 2471-2483.
27. Puzon-McLaughlin, W., Yednock, T.A., and Takada, Y. (1996). Regulation of conformation and ligand binding function of integrin α5β1 by the β1 cytoplasmic domain. J. Biol. Chem. 271, 16580-16585.
28. 1 cytoplasmic domain implicated in cytoskeletal association. J. Cell Biol. 117, 1321-1330.
29. Sakai, T., Zhang, Q.H., Fässler, R., and Mosher, D.F. (1998). Modulation of β1A integrin functions by tyrosine residues in the β1 cytoplasmic domain. J. Cell Biol. 141, 527-538.
30. Shimizu, Y., van Seventer, G.A., Horgan, K.J., and Shaw, S. (1990). Regulated expression and binding of three VLA (β1) integrin receptors on T cells. Nature 345, 250-253.
31. Stewart, M.P., Cabañas, C., and Hogg, N. (1996). T cell adhesion to intercellular adhesion molecule-1 (ICAM-1) is controlled by cell spreading and the activation of integrin LFA-1. J. Immunol. 256, 1810-1817.
32. 3 cytoplasmic domain in triggering focal adhesion kinase phosphorylation. J. Biol. Chem. 272, 7892-7898.
33. van Kooyk, Y., van de Wiel-van Kemenade, P., Weder, P., Kuijpers, T.W., and Figdor, C.G. (1989). Enhancement of LFA-1-mediated cell adhesion by triggering through CD2 or CD3 on T lymphocytes. Nature 342, 811-813.
34. Vignoud, L., Albigés-Rizo, C., Frachet, P., and Block, M.R. (1997). NPXY motifs control the recruitment of the α5β1 integrin in focal adhesions independently of the association of talin with the β1 chain. J. Cell Sci. 110, 1421-1430.
35. 1 integrin does not depend on "NPXY" signals. Biochem. Biophys. Res. Commun. 199, 603-611.
36. Wennerberg, K., Fässler, R., Wärmegård, B., and Johansson, S. (1998). Mutational analysis of the potential phosphorylation sites in the cytoplasmic domain of integrin β1A. Requirement for threonines 788-789 in receptor activation. J. Cell Sci. 111, 1117-1126.
37. Wennerberg, K., Lohikangas, L., Gullberg, D., Pfaff, M., Johansson, S., and Fässler, R. (1996). β1 integrin-dependent and -independent polymerization of fibronectin. J. Cell Biol. 132, 227-238.
38. Yauch, R.L., Felsenfeld, D.P., Kraeft, S.K., Chen, L.B., Sheetz, M.P., and Hemler, M.E. (1997). Mutational evidence for control of cell adhesion through integrin diffusion/clustering, independent of ligand binding. J. Exp. Med. 186, 1347-1355.
39. 3 subunit. Differential effects on cell spreading, recruitment to adhesion plaques, endocytosis, and phagocytosis. J. Biol. Chem. 270, 9550-9557.
40. Zell, T., Hunt, S.W., III, Finkelstein, L.D., and Shimizu, Y. (1996). CD28-mediated upregulation of β1 integrin-mediated adhesion involves phosphatidylinositol 3-kinase. J. Immunol. 156, 883-886.