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Science
Volumn 248, Issue 4960, 1990, Pages 1230-1234
Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI
Author keywords

[No Author keywords available]
Indexed keywords

HERPES; HERPESVIRIDAE; HUMAN HERPESVIRUS 4; ANIMALIA;
EID: 0025335354     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.2161559     Document Type: Article
Times cited : (1013)
References (44)
  • 39
    • 0023902867 scopus 로고
    • 35S]Met with or without TcB2 (170 ng/ml) (13). CSIF was immuno-precipitated with monoclonal antibodies to CSIF as described (12). N-glycanase digestion of immuno-precipitated CSIF was done in the presence of EDTA, SDS, NP-40, and phenylmethylsulfonyl fluoride for 18 to 24 hours at 37°C with N-glycanase (8 unit/ml; GENZYME, Boston, MA). SDS-PAGE (15 to 16% gels) was followed by treatment with Amplify (Amersham, Arlington Heights, IL) as recommended by the manufacturer and autoradiography for 24 to 96 hours.
    • (1988) Mol. Immunol. , vol.25 , pp. 243
    • Peltz, G.1    Frederick, K.2    Anderson, C.L.3    Peterlin, B.M.4
  • 40
    • 0025286538 scopus 로고
    • 5 cells per milliliter in the presence of 1.2 μM ionomycin (Calbiochem, San Diego, CA) (15). Ionomycin stock solution was 1.5 mM in DMSO; controls were cells cultured alone or with only DMSO added. PCR was done for 40 cycles on reverse-transcribed total RNA with synthetic primers from the CSIF sequence that allowed detection of both the 1.5-kb and 1.0-kb mRNA species (primer 1: nucleotides 240 to 270, sense strand; primer 2: nucleotides 630 to 660, antisense strand). Control reactions in which reverse transcriptase was omitted were done for each RNA sample. Amplified fragments were detected initially by ethidium bromide staining in 1.5% agarose gels, and then by hybridization to an oligonucleotide probe (nucleotides 431 to 457, Fig. 3A) to verify specificity of amplification.
    • (1990) J. Immunol. , vol.144 , pp. 1974
    • Gollnick, S.O.1    Trounstine, M.L.2    Yamashita, L.C.3    Kehry, M.R.4    Moore, K.W.5
  • 41
    • 0003799070 scopus 로고
    • DNA sequence analysis was accomplished by the chain-termination method in either M13mpl8/19 or Bluescript KS+ (Stratagene, La Jolla, CA) vectors with the Sequenase 2.0 kit (US Biochemical,Cleveland, OH). The Intelligenetics Suite programs (Intelligenetics, Mountain View, CA) were used for data analysis. DNA blots: 10 to 15 μg of genomic DNA from either sperm (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, or liver was digested with the indicated restriction enzyme, fractionated by electrophoresis through 0.9% agarose gels, blotted, and hybridized (29).
    • (1986) Manipulating the Mouse Embryo , pp. 107
    • Hogan, B.1    Constantini, F.2    Lacy, E.3
  • 42
    • 0019476110 scopus 로고
    • 32P-labeled whole genomic DNA. A mouse CD23 cDNA [S. O. Gollnick et al. in (29)] containing repetitive elements in its 3'-untranslated region was used as a control.
    • (1981) Cell , vol.24 , pp. 125
    • Steinmetz, M.1
  • 43
    • 0022487151 scopus 로고
    • Databases screened were the EMBL nucleic acid sequence bank release 20, Genbank release 61, Swiss Prot protein sequence database release 12, and Protein Identification Resource release 21. No other herpes virus sequences in the databases had any sequence with such a marked relationship to CSIF, although a weakly homologous sequence was found in the varicella zoster virus genome
    • (1986) J. Gen. Virol. , vol.67 , pp. 1759
    • Davison, A.J.1    Scott, J.E.2
  • 44
    • 0023688653 scopus 로고
    • nucleotides 31965 to 32133; 24% identity over 59 amino acids]. The search did not reveal any proteins with significant homology to the ORF in the 3'-untranslated sequence of F115, although we noted an isolated seven amino acid identity between this ORF (nucleotides 1144 to 1164; Fig. 3A) and amino acids 62 to 68 of a mouse T cell receptor V0 sequence
    • (1988) J. Immunol. , vol.140 , pp. 4123
    • McElligott, D.L.1    Sorger, S.B.2    Maris, L.A.3    Hedrick, S.M.4

* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.