Protein P7 of phage Φ6 RNA polymerase complex, acquiring of RNA packaging activity by in vitro assembly of the purified protein onto deficient particles

Journal of Molecular Biology

Volumn 266, Issue 5, 1997, Pages 891-900

  Juuti, Jarmo T ^a   Bamford, Dennis H ^a  

^a NONE

Author keywords

Bacteriophage 6; In vitro assembly; Particle structure; RNA polymerase complex; Transcription regulation

Indexed keywords

DOUBLE STRANDED RNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE; GENOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN PURIFICATION; RNA SYNTHESIS; TRANSCRIPTION REGULATION; VIRION;

EID: 0031567131     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1996.0817     Document Type: Article

References (36)

1. Bartlett, N. M., Gillies, S. C., Bullivant, S. & Bellamy, A. R. (1974). Electron microscopy study of reovirus reaction cores. J. Virol. 14, 315-326.
2. Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal. Biochem. 72, 248-254.
3. Casini, G. & Revel, H. R. (1994). A new small low-abundant nonstructural protein encoded by the L segment of the dsRNA bacteriophage φ6. Virology, 203, 221-228.
4. Casini, G., Qiao, X. & Mindich, L. (1994). Reconstitution of active replicase in procapsids of the segmented dsRNA bacteriophage φ6. Virology, 204, 251-253.
5. Cuppels, D. A., Van Etten, J. L., Burbank, D. E., Lane, L. C. & Vidaver, A. K. (1980). In vitro translation of the three bacteriophage φ6 RNAs. J. Virol. 35, 249-251.
6. Day, L. A. & Mindich, L. (1980). The molecular weight of bacteriophage φ6 and its nucleocapsid. Virology, 103, 376-385.
7. Frilander, M. & Bamford, D. H. (1995). In vitro packaging of the single-stranded RNA genomic precursors of the segmented double-stranded RNA bacteriophage φ6: the three segments modulate each other's packaging efficiency. J. Mol. Biol. 246, 418-428.
8. Frilander, M., Gottlieb, P., Strassman, J., Bamford, D. H. & Mindich, L. (1992). Dependence of minus-strand synthesis on complete genomic packaging in the double-stranded RNA bacteriophage φ6. J. Virol. 66, 5013-5017.
9. Gottlieb, P., Strassman, J., Bamford, D. H. & Mindich, L. (1988). Production of polyhedral particle in Escherichia coli from a cDNA copy of the large genomic segment of bacteriophage φ6. J. Virol. 62, 181-187.
10. Gottlieb, P., Strassman, J., Qiao, X., Frucht, A. & Mindich, L. (1990). In vitro replication, packaging, and transcription of the segmented double-stranded RNA genome of bacteriophage φ6: studies with procapsids assembled from plasmid-encoded proteins. J. Bacteriol. 172, 5774-5782.
11. Gottlieb, P., Strassman, J., Qiao, X., Frilander, M., Frucht, A. & Mindich, L. (1992). In vitro packaging and replication of individual genomic segments of bacteriophage φ6 RNA. J. Virol. 66, 2611-2616.
12. Harlow, E. & Lane, D. (1988). Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
13. Hayes, E. C., Lee, P. W. K., Miller, S. E. & Joklik, W. K. (1981). The interaction of a series of hybridoma IgGs with reovirus particles; demonstration that the core protein λ2 is exposed on the particle surface. Virology, 108, 147-155.
14. Juuti, J. T. & Bamford, D. H. (1995). RNA binding, packaging and polymerase activities of the different incomplete polymerase complex particles of the dsRNA bacteriophage φ6. J. Mol. Biol. 249, 545-554.
15. Kenney, J. M., Hantula, J., Fuller, S. D., Mindich, L., Ojala, P. M. & Bamford, D. H. (1992). Bacteriophage φ6 envelope elucidated by chemical cross-linking, immunodetection, and cryoelectron microscopy. Virology, 190, 635-644.
16. Ktistakis, N. T. & Lang, D. (1987). The dodecahedral framework of the bacteriophage φ6 nucleocapsid is composed of protein P1. J. Virol. 61, 2621-2623.
17. Mindich, L. & Davidoff-Abelson, R. (1980). The characterization of a 120 S particle formed during φ6 infection. Virology, 103, 386-391.
18. Mindich, L., Nemhauser, I., Gottlieb, P., Romantschuk, M., Carton, J., Frucht, S., Strassman, J., Bamford, D. H. & Kalkkinen, N. (1988). Nucleotide sequence of the large double-stranded RNA segment of bacteriophage φ6: genes specifying the viral replicase and transcriptase. J. Virol. 62, 1180-1185.
19. Ojala, P. M., Juuti, J. T. & Bamford, D. H. (1993). Protein P4 of double-stranded RNA bacteriophage φ6 is accessible on the nucleocapsid surface: epitope mapping and orientation of the protein. J. Virol. 67, 2879-2886.
20. Olkkonen, V. M. & Bamford, D. H. (1987). The nucleocapsid of the lipid-containing double-stranded RNA bacteriophage φ6 contains a protein skeleton consisting of a single polypeptide species. J. Virol. 61, 2362-2367.
21. Olkkonen, V. M. & Bamford, D. H. (1989). Quantitation of the adsorption and penetration stages of bacteriophage φ6 infection. Virology, 171, 229-238.
22. Olkkonen, V. M., Pekkala, P. M. & Bamford, D. H. (1988). Monoclonal antibodies to the major structural proteins of bacteriophage φ6. Virology, 165, 317-320.
23. Olkkonen, V. M., Gottlieb, P., Strassman, J., Qiao, X., Bamford, D. H. & Mindich, L. (1990). In vitro assembly of infectious nucleocapsids of bacteriophage φ6: formation of a recombinant double-stranded RNA virus. Proc. Natl Acad. Sci. USA, 87, 9173-9177.
24. Olkkonen, V. M., Ojala, P. M. & Bamford, D. H. (1991). Generation of infectious nucleocapsids by in vitro assembly of the shell protein on to the polymerase complex of the dsRNA bacteriophage φ6. J. Mol. Biol. 218, 569-581.
25. Paatero, A. O., Syväoja, J. E. & Bamford, D. H. (1995). Double-stranded RNA bacteriophage φ6 protein P4 is an unspecific NTPase activated by calcium ions. J. Virol. 69, 6729-6734.
26. Page, C. N., Vajdos, F., Fee, L., Grimsley, G. & Gray, T. (1995). How to measure and predict the molar absorption coefficient of a protein. Protein Sci. 4, 2411-2423.
27. Pagratis, N. & Revel, H. R. (1990). Detection of bacteriophage φ6 minus-strand RNA and novel mRNA isoconformers synthesized in vivo and in vitro, by strand-separating agarose gels. Virology, 177, 273-280.
28. Ralph, S. J., Harvey, J. D. & Bellamy, A. R. (1980). Subunit structure of the reovirus spike. J. Virol. 36, 894-896.
29. Semancik, J. S., Vidaver, A. K. & Van Etten, J. L. (1973). Characterization of a segmented double-helical RNA from bacteriophage φ6. J. Mol. Biol. 78, 617-625.
30. Smith, K. S. (1990). An Introduction to Dynamic Light Scattering by Macromolecules, pp. 58-62, Academic Press Inc., New York.
31. Studier, F. W. & Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-130.
32. Tuma, R., Bamford, J. K. H., Bamford, D. H., Russell, M. P. & Thomas, G. J., Jr (1996). Structure, interactions and dynamics of PDR1 virus I. Coupling of subunit folding and capsid assembly. J. Mol. Biol. 257, 87-101.
33. Van Dijk, A. A., Frilander, M. & Bamford, D. H. (1995). Differentiation between minus and plus-strand synthesis: polymerase activity of dsRNA bacteriophage φ6 in an in vitro packaging and replication system. Virology, 211, 320-323.
34. Van Etten, J., Lane, L., Gonzalez, C., Partridge, J. & Vidaver, A. (1976). Comparative properties of bacteriophage φ6 and φ6 nucleocapsid. J. Virol. 18, 652-658.
35. Vidaver, A. K., Koski, R. K. & Van Etten, J. L. (1973). Bacteriophage φ6: a lipid-containing virus of Pseudomonas phaseolicola. J. Virol. 11, 799-805.
36. Yin, P., Cheang, M. & Coombs, K. M. (1996). The M1 gene is associated with differences in the temperature optimum of the transcriptase activity in reovirus core particles. J. Virol. 70, 1223-1227.